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http://dx.doi.org/10.5352/JLS.2012.22.10.1399

Effects of Hizikia fusiforme Extracts on Adipocyte Differentiation and Adipogenesis in 3T3-L1 Preadipocytes  

Choi, Eun Ok (Department of Food and Nutrition, Dongeui University)
Kim, Hyang Suk (Department of Food and Nutrition, Dongeui University)
Han, Min Ho (Department of Oriental Medicine, Dongeui University)
Choi, Yung Hyun (Anti-aging Research Center, Dongeui University)
Kim, Byung Woo (Blue-Bio Industry Regional Innovation Center, Dongeui University)
Hwang, Jinah (Department of Food and Nutrition, Myongji University)
Hwang, Hye Jin (Department of Food and Nutrition, Dongeui University)
Publication Information
Journal of Life Science / v.22, no.10, 2012 , pp. 1399-1406 More about this Journal
Abstract
The present study was conducted to evaluate the effects of various extracts of Hizikia fusiforme on the anti-obesity effects in 3T3-L1 preadipocytes. We used H. fusiforme extracts from ethanol (EEHF), dichloromethane (CFHF), ethyl acetate (EAFHF), butanol (BFHF), and water (WFHF). Treatment with these extracts significantly suppressed terminal differentiation of 3T3-L1 preadipocytes in a dose-dependent manner as confirmed by a decrease in lipid droplet content through Oil Red O staining; this effect was higher in WFHF than in other extracts. The concentrations of cellular triglyceride were also reduced in 3T3-L1 cells by exposure with these extracts, especially when compared with the controls. Treatment with 200 ${\mu}g/ml$ of WFHF and CFHF caused approximately 42.6% and 23.7% reduction, respectively. In addition, the extracts of H. fusiforme significantly reduced the expression levels of key pro-adipogenic transcription factors, including peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$) and CCAAT/enhancer binding proteins ${\alpha}$ (C/$EBP{\alpha}$) and C/$EBP{\beta}$ as compared with controls. Accordingly, our data indicated that WFHF has a preeminent effect on inhibition of adipocyte differentiation among various extracts, and H. fusiforme extracts may be an ideal candidate for obesity relief.
Keywords
Hizikia fusiforme; 3T3-L1; obesity; adipogenesis; peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$); CCAAT/enhancer binding protein (C/EBP);
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