Browse > Article
http://dx.doi.org/10.5352/JLS.2011.21.8.1190

Effects of Extraction Methods of Medicinal Plants on Human Growth of Neuroblastoma SK-N-SH Cells  

Kwon, Jung-Min (Division of Applied Life Sciences (BK21 programs), Gyeongsang National University)
Moon, Yeon-Gyu (Division of Applied Life Sciences (BK21 programs), Gyeongsang National University)
Kim, Young-Suk (Institute of Agriculture & Life Sciences, Gyeongsang National University)
Jung, Ji-Young (Institute of Agriculture & Life Sciences, Gyeongsang National University)
Ha, Yeong-Lae (Division of Applied Life Sciences (BK21 programs), Gyeongsang National University)
Yang, Jae-Kyung (Institute of Agriculture & Life Sciences, Gyeongsang National University)
Publication Information
Journal of Life Science / v.21, no.8, 2011 , pp. 1190-1198 More about this Journal
Abstract
Extraction methods of medicinal plants were evaluated for growth enhancing effects of human neuroblastoma SK-N-SH cells. Hot-water extraction (reflux for 5 hr), hot-water extraction post treatment (100$^{\circ}C$ or 120$^{\circ}C$, 90 min) and ethanol extraction (reflux for 5 hr) methods were applied to Angelica gigas, Rhemania glutinosa, Paeonia lactiflora and Cnidium officinale samples to extract their constituents. Cells were treated for 2 hr with various concentrations of extracts (0, 0.5, 1, 2, 4 ${\mu}g/{\mu}l}$ media) prior to $H_2O_2$ (250 ${\mu}M$) treatment for 2 hr to provide oxidative stress. Cell viability, caspase-3 expression and apoptosis were measured for cells treated with sample extracts. Hot-water extract exhibited a stronger growth enhancing and apoptosis protecting ability than other extracts. These activities were shown at less than 1 ${\mu}g/{\mu}l}$ concentration, and not greater than 2 ${\mu}g/{\mu}l}$ concentration. Hot-water extract contained more polyphenolic compounds than other extracts coming along with stronger antioxidant activity. The efficacy of antioxidant activity was stronger in the hot-water extract of Angelica gigas than other hot-water extracts of medicinal plants. These results suggest that hot-water extraction is an appropriate method to extract materials for growth enhancing and apoptosis protection of SK-N-SH cells, and hot-water extracts of Angelica gigas might be useful materials for protection from aging brain cells.
Keywords
Apoptosis; Angelica gigas; antioxidant; extraction methods; human neuroblastoma SK-N-SH cells; polyphenols;
Citations & Related Records
Times Cited By KSCI : 11  (Citation Analysis)
연도 인용수 순위
1 Choi, S. Y., S. H. Kim, J. S. Kim, T. Y. Ha, S. R. Kim, K. S. Kang, and I. K. Hwang. 2005. Evaluation of the estrogenic and antioxidant activity of some edible and medicinal plants. Korean J. Food Sci. Technol. 37, 549-556.   과학기술학회마을
2 Deliza, R., A. Rosenthal, F.B.D. Abadio, C.H.O. Silva, and C. Castillo. 2005. Application of high pressure technology in the fruit juice processing: Benefits perceived by consumers. J. Food Eng. 67, 241-246.   DOI
3 Folin, O. and W. Denis. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12, 239-249.
4 Han, S. B., Y. H. Kim, C. W. Lee, S. M. Park, H. Y. Lee, Y. H. Ahn, I. H. Kim, and H. W. Kim. 1998. Characteristic immunostimulation by angelan isolated from Angelica gigas Nakai. Immunopharmacology 40, 39-48.   DOI
5 Hwang, J. B. and M. O. Yang. 2002. Comparison of chemical components of Angelica gigas Nakai and Angelica acuriloba kitagawa. Korea J. Food Sci. Technol. 29, 1113-1118.
6 Islam, M. A., Y. S. Kim, W. J. Jang, S. M. Lee, H. G. Kim, S. Y. Kim, J. O. Kim, and Y. L. Ha. 2008. A mixture of trans, trans conjugated linoleic acid induces apoptosis in MCF-7 human breast cancer cells with reciprocal expression of bax and bcl-2. J. Agric, Food Chem. 56, 5970-5976.   DOI   ScienceOn
7 Kang, Y. H., Y. K. Park, S. R. Oh, and K. D. Moon. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J. Food Sci. Technol. 27, 978-984.   과학기술학회마을
8 Kim, E. Y. I. H. Baik, J. H. Kim, and S. R. Kim. 2009. Isolation and purification of decursin and decurisinol angelate in Angelica gigas Nakai. J. Korean Soc. Food Sci. Nutr. 38, 653-656.   DOI   ScienceOn
9 Ahn, K. S. 1996. A study on the anticancer and immunostimulating agents from the root of Angelica gigas Nakai. Ph.D Disseration, Korea University, Seoul, Korea.
10 Blois, M. S. 2003. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199-1200.
11 Cho, Y. H., B. C. Lee, J. H. Kim, J. H. Kim, H. B. Pyo, Y. H. Zhang, and H. D. Park. 2005. Effect of Artemisia anomala S. Moore on antioxidant activity and melanogenesis. Korean J. Pharmacogn. 36, 273-277.   과학기술학회마을
12 Rakib, Md. A., Y. S. Kim, W. J. Jang, B. D. Choi, J. O. Kim, I. K. Kong, and Y. L. Ha. 2010. Attenuation of 12-O-tetradecanoylphorbol- 13-acetate (TPA)-induced gap junctional intercellular communication (GJIC) inhibition in MCF-10A cells by c9,t11-conjugated linoleic acid. J. Agric. Food Chem. 58, 12022-12030.   DOI
13 Shin, S., J. H. Jeon, D. Park, J. Y. Jang, S. S. Joo, B. Y. Hwang, S. Y. Choe, and Y. B. Kim. 2009. Anti-inflammatory effects of an ethanol extract of Angelica gigas in a carrageenan-air pouch inflammation model carrageenan-air pouch inflammation model. Exp. Anim. 58, 431-436.   DOI
14 So, Y. K., Y. L. Ki, H. S. Sang, and C. K. Young. 2005. Four new neuroprotective dihydropyranocoumarins from Angelica gigas. J. Nat. Prod. 68, 56-59.   DOI
15 Sohn, E. S., S. W. Kim, Kang, and S. P. Lee. 2004. Domestic R&D trend analysis of functional food using medical plants. Applied Chem. 8, 470-473.
16 Turkmen, N., F. Sari, and Y. Sedat Velioglu. 2006. Effect of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chem. 99, 835-841.   DOI
17 Yu, H. H., S. J. Seo, Y. H. Kim, H. Y.Lee, R. K. Park, H. S. So, S. l. Jang, and Y. O. You. 2006. Protective effect of Rehmannia glutinosa on the cisplatin-induced damage of HEI-OCI auditory cells through scavenging free radicals. J. Ethnopharmacol. 107, 383-388.   DOI
18 Locatelli, M., F. Travaglia, J. D. Coisson, A. Martelli, C. Stevigny, and M. Arlorio. 2010. Total antioxidant activity of hazelnut skin (Nocciola Piemonte PGI): Impact of different roasting conditions. Food Chem. 119, 1647-1655.   DOI
19 Maritinez, S., L. Valek, J. Piljac, and M. Metikos-Jukovic. 2005. Determination of wine antioxidant capacity by derivative potentiometric titration with electrogenerated cholorine. Eur. Food Res. Technol. 220, 658-661.   DOI
20 Moon, J. S., J. S. Park, Y. M. Park, Y. S. Hwang, E. H. Kim, J. W. Park, I. B. Park, S. W. Kim, S. G. Kang, Y. K. Park, and S. T. Jung. 2008. Antioxidant activaties of methanol extracts from prunella uulgaris. J. Korean Soc. Food Sci. Nutr. 37, 1535-1541.   DOI
21 Nam, S. H. and M. Y. Kang. 2000. Screening of antioxidative activity of hot-water extracts from medicinal plants. J. Korean Soc. Agric. Chem. Biotechnol. 43, 141-147.   과학기술학회마을
22 Park, Y. S. 2002. Antioxidative activities and contents of polyphenolic compound of medicinal herb extracts. J. Easat Asian Soc. Dietary Life 12, 23-31.   과학기술학회마을
23 Park, C., H. S. So, S. H. Shin, J. Y. Choi, I. Lee, J. K. Kim, S. Y. Chung, and R. Park. 2007. The water extract of Omija protects H9c2 cardiomyoblast cells from hydrogen peroxide. Phytother. Res. 21, 81-88.   DOI
24 Salikhova, R. A. and G. G. Poroshenko. 1995. Antimutagenic properties of Angelica archangelica L. Vestn. Ross. Akad. Med. Nauk 1, 58-61.
25 Santos-Buelga, C., G. Williamson, and M. Saltmarsh. 2003. Methods in polyphenol analysis. pp. 1-16, Royal Society of Chemistry, Cambridge, England.
26 Seo, H. Y., J. H. Kim, H. P. Song, D. H. Kim, M. W. Byun, J. H. Kwon, and K. S. Kim. 2007. Effects of gamma irradiation on the yields of volatile extracts of Angelica gigas Nakai. Int. J. Radiat. Phys. Chem. 76, 1869-1874.   DOI   ScienceOn
27 Kim, D. H., W. Y. Jung, S. J. Park, J. M. Kim, S. Lee, Y. C. Kim, and J. H. Ryu. 2010. Anti-amnesic effect of ESP-102 on A$\beta$1-42-induced memory impairment in mice. Pharmacol. Biochem. Behav. 97, 239-248.   DOI
28 Kim, Y. S., R. M. Cerbo, C. K. Hah, K. N. Bahn, J. O. Kim, and Y. L. Ha. 2008. Growth inhibition of osteosarcoma cell MG-63 by a mixture of trans, trans conjugated linoleic aicd isomers: possible mechanistic actions. J. Food Sci. 73, T7-15.   DOI   ScienceOn
29 Kim, M. R., I. H. Kim, and J. H. Shim. 2005. The analysis of volatile components of fresh ginseng, red ginseng and white ginseng by solvent free solid injector (SFSI) techniques. Korean J. Environ. Agric. 24, 164-168.   과학기술학회마을   DOI
30 Kim, Y. C., H. D. Hong, J. H. Rho, C. W. Cho, Y. K. Rhee, and J. H. Yim. 2007. Changes of phenolic acid contents and radical scavenging activities of ginseng according to steaming times. Korean J. Ginseng Res. 31, 230-236.   과학기술학회마을   DOI
31 Koo, U., H. J. Lee, D. H. Lee, H. J. Lee, A. R. Ham, E. Y. Cho, and W. Mar. 2008. Anti-oxidative effect of some plant extracts against nitric oxide-induced oxidative stress on neuronal cell. Korean J. Pharmacogn. 39, 290-294.   과학기술학회마을
32 Korea Institute for Health and Social Affairs, http://www.kihasa.re.kr.
33 Lee, S. H., Y. S. Lee, S. H. Jung, K. H. Shin, B. K. Kim, and S. S. Kang. 2003. Anti-tumor activities of decursinol angelate and decursin from Angelica gigas. Arch. Pharm. Res. 26, 727-730.   과학기술학회마을   DOI
34 Li, L., W. Li, S. W. Jung, Y. W. Lee, and Y. H. Kim. 2011. Protective effects of decursin and decursinol angelate against amyloid $\beta$-protein-induced oxidative stress in the PC12 cell line: The role of Nrf2 and antioxidant enzymes. Biosci. Biotechnol. Biochem. 75, 434-442.   DOI