Browse > Article
http://dx.doi.org/10.5352/JLS.2011.21.8.1120

Anti-Oxidant and Antiinflammatory Effects of Rosa multiflora Root  

Park, Geun-Hye (Department of Cosmeceutical Science, Daegu Haany University)
Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University)
Kim, Dong-Hee (Department of Cosmeceutical Science, Daegu Haany University)
Cho, Young-Je (Department of Food Engineering, Gyungbuk National University)
An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University)
Publication Information
Journal of Life Science / v.21, no.8, 2011 , pp. 1120-1126 More about this Journal
Abstract
Rosa multiflora thunberg belonging to Rosaceae is widely distributed in East Asia including Korea and Japan, and has been reported to have tormentic acid and rosamultin. To develop a new natural anti-inflammatory agent for cosmetics, we investigated the inhibitory effects of inflammation in Rosa multiflora root (R. multiflora root). The biological activity and anti-inflammatory effects were investigated by water, ethanol, methanol and acetone extracts of R. multiflora root. The measurements of polyphenol content from R. multiflora root were highest in water and acetone extracts, at 57.48 ${\pm}$ 0.88 mg/g and 67.05 ${\pm}$ 0.56 mg/g, respectively. The result of DPPH, ABTS and superoxide anion radical scavenging effects showed over 50% efficacy at 50 ${\mu}g/ml$ in ethanol, methanol and acetone extracts. Hyaluronidase inhibition effect showed over 60% efficacy at 500 ${\mu}g/ml$ in ethanol, methanol, and acetone extracts. Nitric oxide radical inhibition effect of R. multiflora root ethanol extracts showed over 30% efficacy at 500 ${\mu}g/ml$. We investigated the effect of R. multiflora root extracts on nitric oxide (NO) production of inducible nitric oxide synthase (iNOS) in LPS-induced RAW 264.7 macrophage cells. The result showed that R. multiflora root extracts have an inhibitory effect on NO production and iNOS expression and also can be used as an anti-inflammatory agent. These antioxidant and anti-inflammatory effects of R. multiflora root show applicant potential application as a functional cosmetic material.
Keywords
Rosa multiflora root; antioxidant effect; anti-inflammatory; nitric oxide (NO); inducible nitric oxide synthase (iNOS);
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Okamura, H., A. Mimura, Y. Yakou, M. Niwano, and Y. Takahashi. 1993. Antioxidant activity of tannins and flavonoids in Eucalyptus rostrata. Phytochemistry 33, 557-561.   DOI
2 Pellegrini, N., A. Proteggente, A. Pannala, M. Yang, R. Re, and C. Rice-Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26, 1231-1237.   DOI
3 Pratt, D. E., M. T. Huang, S. T. Ho, and C. Y. Lee. 1992. In Phenolic compound in food and their effects on health (II), Antioxidants and Cancer Prevention. pp. 54-71, Washington DC.
4 Reissig, J. L., J. L. Storminger, and L. F. Leloir. 1995. A modified colorimetric method for the estimation of N-acetylamino sugars. J. Biol. Chem. 217, 959-966.
5 Stuehr, D. J., H. J. Cho, N. S. Kwon, M. F. Weise, and C. F. Nathan. 1991. Purification and characterization of the cytokine- induced macrophage nitric oxide synthase: an FADand FMN-containing flavoprotein. Proc. Natl. Acad. Sci. 88, 7773-7777.   DOI
6 Byun, S. H., C.H. Yang, and S. C. Kim. 2005. Inhibitory effect of Scrophulariae Radix extract on $TNF-{\alpha}$, $IL-1{\beta}$, IL-6 and nitric oxide production in lipopolysaccharide-activated Raw 264.7 cells. Korean J. Herbology 20, 7-16.   과학기술학회마을
7 Carmichael, J., W. G. DeGraff, A. F. Gazdar, J. D. Minna, and J. B. Mitchel. 1987. Evaluation of a tetrazolium based semiautomated colorimetric assay : assessment of chemosensitivity testing. Cancer Res. 47, 936-942.
8 Choi, W. Y., H. J. Chun, J. H. Lee, and S. H. Baek. 2003. Effect of methanol extract from Cornis fructus on melanogenesis. Korean J. Pharmacogn. 31, 70-74.
9 Folin, O. and W. Denis. 1912. On phosphotungastic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12, 239-249.
10 Fridovich, I. 1970. Quantitative aspects of the production of superoxide anion radical by milk xanthin oxidase. J. Biol. Chem. 245, 4053-4057.
11 Han, J. T. 2006. Development of functional material using the root of Rosamultiflora. Food Industry Nutrition 11, 59-65.
12 Kim, J. K. and H. S. Lee. 2000. Tyrosinase inhibitory and radical scavenging activities for the seeds of Coix. Korean J. Food Sci. Technol. 32, 1409-1413.   과학기술학회마을
13 Jeong, I. Y. 2005. Antioxidant activity and radio protection of two flavonoids from propolis. J. Korean Soc. Food Sci. Nutr. 34, 162-166.   DOI
14 Jung, B. S. and M. G. Shin. 1990. DoGam HangYak Daesajeon. pp. 648-649, Young Rim Sa, Korea.
15 Jung, S. J., J. H. Lee, H. N. Song, N. S. Seong, S. E. Lee, and N. I. Baek. 2004. Screening for antioxidant of plant medicinal extract. J. Korean Soc. Appl. Biol. Chem. 47, 135-140.
16 Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1120.
17 Amakura, Y., M. Umono, S. Tsuji, H. Ito, T. Hatano, T. Yoshida, and Y. Tonogai. 2002. Constituents and their antioxidative effect in eucalyptus leaf extract used as a natural food additive. Food Chemistry 77, 47-56.   DOI
18 An, B. J. and J. T. Lee. 2002. Studies on biologlcal activity from extract of Crataegi fructus. Korean J. Herbology 17, 29-38.
19 Black, H. S. 1987. Potential involvement of free radical reaction in ultraviolet light-mediated cutaneous damage. Photochem. Photobiol. 46, 213-221.   DOI