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http://dx.doi.org/10.5352/JLS.2011.21.8.1100

Ameliorating Effects of Moxifloxacin on Endotoxin-Induced Acute Lung Injury in Rats  

Lee, Young-Man (Department of Physiology, Daegu Catholic University)
Chae, Whi-Gun (Department of Biochemistry, School of Medicine, Daegu Catholic University)
Publication Information
Journal of Life Science / v.21, no.8, 2011 , pp. 1100-1108 More about this Journal
Abstract
The immunomodulating effects of moxifloxacin seem to be effective in downregulating inflammatory reactions. This presumed effect was tested in endotoxin (ETX)-induced acute lung injury (ALI) in rats. After moxifloxacin treatment (10 mg/kg) of ETX-given rats, lung myeloperoxidase (MPO) activity, bronchoalveolar-lavage (BAL) protein, and the number of neutrophils in the BAL cells were measured. Light and electron microscopic structures were also examined. Electron microscopic $CeCl_3$ histochemistry for the detection of hydrogen peroxide in the lungs and immunohistochemistry of cytosolic phospholipase A2 (cPLA2) in the lung tissues and BAL cells were performed. To examine the expression of TNF${\alpha}$ in the lungs, western blotting was carried out with the lung tissues. ETX had accumulated neutrophils in the lungs, which was followed by lung leak. Oxidative stress occurred, and increased expression of cPLA2 in the lung tissues and BAL cells was observed in the ETX-given rats. Simultaneously, the expression of TNF${\alpha}$ was enhanced by ETX. Moxifloxacin, however, decreased all these parameters, indicating that ALI may have been ameliorated. Moxifloxacin appears to ameliorate ETX-induced ALI partially through the suppression of cPLA2 in the lungs of rats.
Keywords
Endotoxin; induced acute lung injury (ALI); cytosolic PLA2; moxifloxacin;
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