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http://dx.doi.org/10.5352/JLS.2011.21.6.907

Production of Recombinant Human Keratinocyte Growth Factor from Bombyx mori (Lepidopera: Bombycidae) Bm5 Cells  

Han, Song-Yi (Department of Anatomy, School of Medicine, Chungnam National University)
Jin, Cho-Yi (Department of Anatomy, School of Medicine, Chungnam National University)
Kwon, Ki-Sang (Department of Anatomy, School of Medicine, Chungnam National University)
Yun, Eun-Young (Department of Sericulture & Entomology, National Institute of Agriculture Science and Technology, R.D.A.)
Goo, Tae-Won (Department of Sericulture & Entomology, National Institute of Agriculture Science and Technology, R.D.A.)
Kim, Seung-Whan (Department of Emergency Medicine, Chungnam National University Hospital)
Choi, Jong-Soon (Division of Life Science, Korea Basic Science Institute)
Yu, Kweon (Aging Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kwon, O-Yu (Department of Anatomy, School of Medicine, Chungnam National University)
Publication Information
Journal of Life Science / v.21, no.6, 2011 , pp. 907-911 More about this Journal
Abstract
Using silkworm Bombyx mori Bm5 cells, we established a stable cell line expressing the human keratinocyte growth factor (hKGF), named by the Bm5-hKGF cell, in which the protein hKGF is synthesized in the cell and secreted in the cell culture supernatant (CCS) at approximately 15-20 ng/ml. When the Bm5-hKGF cell was co-expressed with B. mori protein disulfide isomerase (bPDI) cDNA, its secretion increased by about two times the original amount. Through wound healing migration assay, it was demonstrated that the secreted hKGF included in the CCS has a very powerful biological activity of keratinocyte proliferation. We expect to produce useful human recombinant proteins from silkworm cultured cells in large quantities at low prices.
Keywords
Silkworm Bombyx mori Bm5 cells; human keratinocyte growth factor; protein disulfide isomerase;
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Times Cited By KSCI : 1  (Citation Analysis)
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