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http://dx.doi.org/10.5352/JLS.2011.21.5.656

Anti-Inflammatory Effect of Licochalcone E, a Constituent of Licorice, on Lipopolysaccharide-Induced Inflammatory Responses in Murine Macrophages  

Park, Geun-Mook (Department of Biomedical Science, College of Natural Science, Catholic University of Daegu)
Jun, Jong-Gab (Department of Chemistry and Institute of Natural Medicine, Hallym University)
Kim, Jin-Kyung (Department of Biomedical Science, College of Natural Science, Catholic University of Daegu)
Publication Information
Journal of Life Science / v.21, no.5, 2011 , pp. 656-663 More about this Journal
Abstract
Licochalcone, a major phenolic constituent of the licorice species Glycyrrhiza inflata, a constituent of licorice, exhibits various biological properties, including chemopreventive-, antibacterial-, and anti-spasmodic activities. Recently, Licochalcone E (LicE) was isolated from the roots of Glycyrrhiza inflate, however its biological functions have not been fully examined. In the present study, we investigated the ability of LicE to regulate inflammation reactions in macrophages. Our in vitro experiments using murine macrophages, RAW264.7 cells, showed that LicE suppressed not only nitric oxide (NO) and prostaglandin $E_2$ generation, but also the expression of inducible NO synthase and cyclooxygenase-2 induced by lipopolysaccharide (LPS). Similarly, LicE inhibited the release of proinflammatory cytokines induced by LPS in RAW264.7 cells, including tumor necrosis factor-${\alpha}$ and interleukin-6. The underlying mechanism of LicE on anti-inflammatory action correlated with down-regulation of the nuclear factor-${\kappa}$B. Our data collectively indicate that LicE inhibited the production of several inflammatory mediators and might be used in the treatment of various inflammatory diseases.
Keywords
Inflammation; licochalcone E; NF-${\kappa}$B; macrophage; inflammatory mediators;
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