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http://dx.doi.org/10.5352/JLS.2011.21.4.549

Novel Method for Urinary 1-Hydroxypyrene Measurement Using Molecular Imprinting  

Yim, Dong-Hyuk (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University)
Moon, Sun-In (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University)
Choi, Young-Sook (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University)
Park, Hee-Jin (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University)
Kim, Dae-Seon (Environmental Health Research Division, Environmental Health Research Department, National Institute of Environmental Research)
Yu, Seung-Do (Environmental Health Research Division, Environmental Health Research Department, National Institute of Environmental Research)
Lee, Chul-Ho (Asbestos Damage Relief Center, Korea Environment Coporation)
Kim, Yong-Dae (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University)
Kim, Heon (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University)
Publication Information
Journal of Life Science / v.21, no.4, 2011 , pp. 549-553 More about this Journal
Abstract
This study was performed to determine whether or not urinary 1-hydroxypyrene (1-OHP) levels can be accurately detected by our 1-OHP-detecting $TiO_2$-Bead-HPLC assay that we developed based on the molecular imprinting method. Our method showed a variation coefficient of 4.97% and a between-day variation coefficient of 4.43%, suggesting that this may be a very stable method. In addition, the recovery rate of 1-OHP from a mixture of 1-OHP and similar substances using our $TiO_2$-Bead-HPLC method was estimated to be 105.6%. The correlation coefficient between the conventional enzyme-HPLC method and this new method was 0.74 (p<0.01) when the urine samples were tested. Based on this result, it is conceivable that our method could be a useful technique for measuring urinary 1-OHP levels. Moreover, our method has some advantages of being easier and less expensive than the conventional method. The results of this study suggest that our method can facilitate the development of a urine 1-OHP sensor using $TiO_2$-coating beads and that development of beads by molecular imprinting can be applied to analysis of chemicals other than 1-OHP.
Keywords
$TiO_2$ bead; molecular imprinting; 1-hydroxypyrene;
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