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http://dx.doi.org/10.5352/JLS.2011.21.4.486

Involvement of Peroxynitrite in NO Donor-Induced HO-1 Expression in Rat Articular Chondrocytes  

Song, Ju-Dong (Department of Microbiology and Immunology, Pusan National University School of Medicine)
Kim, Kang-Mi (Department of Microbiology and Immunology, Pusan National University School of Medicine)
Kim, Jong-Min (Department of Anatomy and Cell Biology, Dong-A University College of Medicine)
Yoo, Young-Hyun (Department of Anatomy and Cell Biology, Dong-A University College of Medicine)
Park, Young-Chul (Department of Microbiology and Immunology, Pusan National University School of Medicine)
Publication Information
Journal of Life Science / v.21, no.4, 2011 , pp. 486-493 More about this Journal
Abstract
Nitric oxide (NO) donors are a potent inducer of heme oxygenase-1 (HO-1). However, it is unclear whether or not HO-1 expression induced by NO donors is a direct consequence of NO released by NO donors. Here, we investigated the effects of NO donors on the expression of HO-1 in primary rat articular chondrocytes. NO donors (SIN-1, SNAP, and SNP) significantly induced the accumulation of HO-1 protein accompanied by an increase in HO-1 mRNA. NO donor-induced HO-1 expression exerted cytoprotection against NO and/or superoxide-induced cell death. Guanylate cyclase signaling was not associated with Nrf2 and HO-1 expression in NO donor-treated chondrocytes. Interestingly, NO scavenger carboxy-PTIO and SOD mimetic TEMPOL markedly inhibited NO donor-induced HO-1 expression in chondrocytes. In addition, NO donor-induced HO-1 expression was completely abrogated by the peroxynitrite scavenger MnTBAP. Since peroxynitrite can be physiologcally formed in the cell through reaction of NO with superoxide, we analyzed whether or not peroxynitrite could directly induce HO-1 expression in chondrocytes. Peroxynitrite treatment in chondrocytes evoked doseand time-dependent Nrf2 and HO-1 expression. These results indicate that HO-1 expression induced by NO donors in rat articular chondrocytes is due to NO-mediated peroxynitrite rather than NO.
Keywords
Nitric oxide (NO) donors; peroxynitrite; Nrf2; heme oxygenase-1 (HO-1); chondrocytes;
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