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http://dx.doi.org/10.5352/JLS.2011.21.1.155

Association of a Single Nucleotide Polymorphism with Economic Traits in Porcine Uncoupling Protein 3 Gene  

Oh, Jae-Don (Genomic Informatics Center, Hankyong National University)
Lee, Kun-Woo (Genomic Informatics Center, Hankyong National University)
Jung, Il-Jung (Genomic Informatics Center, Hankyong National University)
Jeon, Gwang-Joo (Genomic Informatics Center, Hankyong National University)
Lee, Hak-Kyo (Genomic Informatics Center, Hankyong National University)
Kong, Hong-Sik (Genomic Informatics Center, Hankyong National University)
Publication Information
Journal of Life Science / v.21, no.1, 2011 , pp. 155-158 More about this Journal
Abstract
Uncoupling protein (UCP) 3 has a number of proposed roles in the regulation of fatty acid metabolism. A number of polymorphisms in the human UCP3 gene have been identified, and the correlation with obesity related phenotypes evaluated. The objective of this study was to identify SNP in porcine UCP3 gene and to investigate the effect of the SNP on economic traits. The sequencing analysis method was used to identify nucleotide polymorphisms at position 1405 bp (Genebank accession No : AY739704) in porcine UCP3 gene. The SNP (G150R), located in the exon 3, changed the amino acid to glycine (GGG) from arginine (AGG). This G150R showed three genotypes - GG, GR and RR - by digestion with the restriction enzyme Sma Ⅰ using the PCR-RFLP method. The G150R showed significant effects only on back fat (P<0.05). Animals with the genotype GG had significantly higher back fat thickness (1.358 cm) than animals with the genotype GR (1.288 cm, P<0.05) and RR (1.286 cm, P<0.05). However, the genotypes had no significant association with ADG and days to 90kg. According to results of this study, a G allele of the G150R was found to have a significant effect on back fat thickness. It will be possible to use SNP markers on selected pigs to improve backfat thickness, an important economic trait.
Keywords
Uncoupling protein 3 (UCP3); SNP; backfat thickness; pig;
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1 Walder, K., R. A. Norman, R. L. Hanson, P. Schrauwen, M. Neverova, C. P. Jenkinson, J. Easlick, C. H. Warden, C. Pecqueur, S. Raimbault, D. Ricquier, M. H. Silver, A. R. Shuldiner, G. Solanes, B. B. Lowell, W. K. Chung, R. L. Leibel, R. Pratley, and E. Ravussin. 1998. Association between uncoupling protein polymorphisms (UCP2-UCP3) and energy metabolism/obesity in Pima Indians. Hum. Mol. Genet. 7, 1431-1435.   DOI
2 Wang, S., A. Subramaniam, M. A. Cawthorne, and J. C. Clapham. 2003. Increased fatty acid oxidation in transgenic mice overexpression UCP3 in skeletal muscle. Diabetes, Obesity and Metabolism 5, 295-301.   DOI
3 Weigle, D. S., L. E. Selfridge, M. W. Schwartz, R. J. Seeley, D. E. Cummings, P. J. Havel, J. L. Kuijper, and H. BeltrandelRio. 1998. Elevated free fatty acids induce uncoupling protein 3 expression in muscle: a potential explanation for the effect of fasting. Diabetes 47, 298-302.   DOI
4 Himms-Hagen, J. and M. Harper. 2001. Physiological role of UCP3 may be export of fatty acids from mitochondria when fatty acid oxidation predominates: an hypothesis. Exp. Biol. Med. 226, 78-84.
5 Hoeks, J., M. A. van Baak, M. K. Hesselink, G. B. Hul, H. Vidal, W. H. Saris, and P. Schrauwen. 2003. Effect of _1- and _2-adrenergic timulation on energy expenditure, substrate oxidation, and UCP3 expression in humans. Am. J. Physiol. Endocrinol. Metab. 285, E775-E782.
6 Muzzin, P., O. Boss, and J. P. Giacobino. 1999. Uncoupling protein 3: its possible biological role and mode of regulation in rodents and humans. J. Bioenerg. Biomembr. 31, 467-473.   DOI
7 Otabe, S., K. Clement, S. Dubois, F. Lepretre, V. Pelloux, R. Leibel, W. Chung, P. Boutin, B. Guy-Grand, P. Froguel, and F. Vasseur. 1999. Mutation screening and association studies of the human uncoupling protein 3 gene in normoglycemic and diabetic morbidly obese patients. Diabetes 48, 206-208.
8 Schrauwen, P., W. H. M. Saris, and M. K. C. Hesselink. 2001. An alternative function for human uncoupling protein 3: protection of mitochondria against accumulation of nonesterified fatty acids inside the mitochondrial matrix. FASEB J. 15, 2497-2502.   DOI
9 Simonsen, L., B. Stallknecht, and J. Bulow. 1993. Contribution of skeletal muscle and adipose tissue to adrenaline-induced thermogenesis in man. Int. J. Obes. Relat. Metab. Disord. 17, S47-S51.
10 Spiegelman, B. M. and J. S. Flier. 2001. Obesity and the regulation of energy balance. Cell 104, 531-543.   DOI
11 Urhammer, S. A., L. T. Dalgaard, T. I. Sørensen, A. Tybjaerg-Hansen, S. M. Echwald, T. Andersen, J. O. Clausen, and O. Pedersen. 1998. Organisation of the coding exons and mutational screening of the uncoupling protein 3 gene in subjects with juvenile-onset obesity. Diabetologia 41, 241-244.   DOI
12 Cline, G. W., A. J. Vidal-Puig, S. Dufour, K. S. Cadman, B. B. Lowell, and G. I. Shulman. 2001. In vivo effects of uncoupling protein-3 gene disruption on mitochondrial energy metabolism. J. Biol. Chem. 276, 20240-20244.   DOI
13 Bao, S., A. Kennedy, B. Wojciechowski, P. Wallace, E. Ganaway, and W. T. Garvey. 1998. Expression of mRNAs encoding uncoupling proteins in human skeletal muscle. Effects of obesity and diabetes. Diabetes 47, 1935-1940.   DOI
14 Boss, O., E. Bobbioni-Harsch, F. Assimacopoulos-Jeannet, P. Muzzin, R. Munger, J. P. Giacobino, and A. Golay. 1998. Uncoupling protein-3 expression in skeletal muscle and free fatty acids in obesity. Lancet 351, 1933.
15 Brun, S., M. C. Carmona, T. Mampel, O. Vinas, M. Giralt, R. Iglesias, and F. Villarroya. 1999. Uncoupling protein-3 gene expression in skeletal muscle during development is regulated by nutritional factors that alter circulating non-esterified fatty acids. FEBS Letters 453, 205-209.   DOI
16 Dalgaard, L. T. and O. Pedersen. 2001. Uncoupling proteins: functional characteristics and role in the pathogenesis of obesity and Type II diabetes. Diabetologia 44, 946-965.   DOI
17 Damon, M., A. Vincent, A. Lombardi, and P. Herpin. 2000. First evidence of uncoupling protein-2 (UCP-2) and -3 (UCP-3) gene expression in piglet skeletal muscle and adipose tissue. Gene 246, 133-141.   DOI