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http://dx.doi.org/10.5352/JLS.2011.21.1.127

Characteristics of Lactose Hydrolysis by Immobilized β-Galactosidase on Chitosan Bead  

Kang, Byung-Chul (Department of Chemical Engineering, Dong-Eui University)
Publication Information
Journal of Life Science / v.21, no.1, 2011 , pp. 127-133 More about this Journal
Abstract
${\beta}$-Galactosidase was immobilized on chitosan bead by covalent bonding using glutaraldehyde. The characteristics of the immobilized enzyme were investigated. Maximum immobilization yield of 75% was obtained on chitosan bead. Optimum pH and temperature for the immobilized enzyme was 7.0 and $50^{\circ}C$, respectively. The immobilized enzyme showed a broader range of pH and temperature compared to a free one. A mathematical model for the operation of the immobilized enzyme in a packed-bed reactor was established and solved numerically. Under different inlet lactose concentrations and feed flow rate conditions, lactose conversion was measured in a packed-bed reactor. The experimental results of continuous operation in a packed-bed reactor were compared to theoretic results using Michaelis-Menten kinetics with competitive product inhibition and external mass transfer resistance. The model predicted the experimental data with errors less than 5%. Process optimization of continuous operation in a packed-bed reactor was also conducted. In a recirculation packed-bed operation, conversion of lactose was 97% in 3 hours. In a continuous packed-bed operation, the effect of flow rate and initial lactose concentration was investigated. Increasing flow rates and initial lactose concentration decreased the conversion of substrate.
Keywords
Packed-bed reactor; lactose hydrolysis; galactosidase; mathematical model;
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