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http://dx.doi.org/10.5352/JLS.2011.21.12.1784

miR-7b Promoter Contains Negative Gene Elements  

Choi, Ji-Woong (Department of Oral Microbiology, School of Dentistry)
Lee, Heon-Jin (Brain Science and Engineering Institute, Kyungpook National University)
Publication Information
Journal of Life Science / v.21, no.12, 2011 , pp. 1784-1788 More about this Journal
Abstract
The typical miRNA and its nearby host gene are co-expressed by sharing the same promoter. We assumed that miR-7b and its host gene FICT might use an identical promoter for their brain specific gene expression. Sequence comparison of the genomic DNA of mouse miR-7b, human miR-7-3 and their host genes by using the bioinformatic tools revealed high sequence homology and several putative transcription factor-binding sites on the promoter region. In order to probe the hypothesis we used a luciferase vector system into which we cloned the 5' upstream conserved region of miR-7b and FICT. The putative promoter region showed decreased luciferase activity, suggesting that the 5' upstream of miR-7b and FICT contain a negative regulator for gene expression.
Keywords
miRNA; promoter; gene regulation; DNA element; cis element;
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