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http://dx.doi.org/10.5352/JLS.2011.21.11.1579

Inhibition of α-Glucosidase by a Semi-Purified Ethyl Acetate Fraction from Submerged-Liquid Culture of Agaricus blazei Murill  

Jung, Kwan-Ju (Division of Applied Life Science (BK21 programs), Institute of Agriculture and Life Science, Graduate School of Gyeongsang National University)
Moon, Yeon-Gyu (Division of Applied Life Science (BK21 programs), Institute of Agriculture and Life Science, Graduate School of Gyeongsang National University)
Kwon, Jung-Min (Division of Applied Life Science (BK21 programs), Institute of Agriculture and Life Science, Graduate School of Gyeongsang National University)
Ahn, Chae-Rin (Division of Applied Life Science (BK21 programs), Institute of Agriculture and Life Science, Graduate School of Gyeongsang National University)
Kim, Jeong-Ok (HK Biotech Co., Ltd.)
Ha, Yeong-Lae (Division of Applied Life Science (BK21 programs), Institute of Agriculture and Life Science, Graduate School of Gyeongsang National University)
Publication Information
Journal of Life Science / v.21, no.11, 2011 , pp. 1579-1585 More about this Journal
Abstract
Natural anti-diabetic semipurified ethyl acetate fraction was isolated from the submerged-liquid culture of Agaricus blaze Murill (AB) in a medium containing soybean flakes. Hot-water extract of AB (HEAB) was prepared by extraction at $121^{\circ}C$ for 60 min, followed by filtering through a filter presser filled with diatomate. The ${\beta}$-glucan-free HEAB, which was a supernatant fraction from HEAB by precipitation in an 80% ethanol solution, was fractionated into hexane, chloroform, ethyl acetate, and butanol fractions. The inhibition of the ${\alpha}$-glucosidase activity by fractions was 59.0, 17.0, 61.6, and 37.9%, respectively, suggesting that ethyl acetate fraction was the most active. A subfraction having a strong ${\alpha}$-glucosidase inhibitory activity (80.4%) was isolated from the ethyl acetate fraction. This subfraction contained isoflavones (genistin and daidzin) and their conjugates with sugars as potent inhibiters of ${\alpha}$-glucosidase activity. These results suggest that the ethyl acetate fraction or HEAB containing isoflavones and their sugars conjugates could be useful sources for controlling blood sugar levels in humans.
Keywords
Agaricus blazei Murill (AB); ${\alpha}$-glucosidase; isoflavone; isoflavone conjugates with sugars;
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