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http://dx.doi.org/10.5352/JLS.2011.21.10.1466

Characterization of the Promoter Controling the Stage-Specific Gene Expression of Bombyx mori  

Park, Seung-Won (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Choi, Gwang-Ho (Research Policy Planning Division, Rural Development Administration)
Goo, Tae-Won (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Kim, Seong-Ryul (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Kang, Seok-Woo (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Publication Information
Journal of Life Science / v.21, no.10, 2011 , pp. 1466-1472 More about this Journal
Abstract
We characterized embryo early gene (EEG)-704 promoter of the silkworm Bombyx mori, which is specifically regulated in the development stages. To determine core promoter region, 10 different partial mutant clones were tested by luciferase assay in Sf9 cells. About 1.5 kb promoter shows higher luciferase activity than constitutive promoter (BmA3). Interestingly, EEG-704 shares the same DNA sequences with BmHsp20.8 by the result of BLAST analysis; its expression is also increased under heat shock condition. Development of such promoter inducible, directly or indirectly in the developmental-stage, is very useful in making recombinant proteins in transgenic silkworms.
Keywords
Bombyx mori; embryo early gene; promoter; luciferase assay;
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