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http://dx.doi.org/10.5352/JLS.2011.21.10.1355

Aged Garlic Extract and Its Components Inhibit Platelet Aggregation in Rat  

Choi, You-Hee (College of Pharmacy and Research Institute of Drug Development, Chonnam National University)
Jeong, Hyung-Min (College of Pharmacy and Research Institute of Drug Development, Chonnam National University)
Kyung, Kyu-Hang (Deptment of Food Science, Sejong University)
Ryu, Beung-Ho (DOUL Agircultural Farming Corp.)
Lee, Kwang-Youl (College of Pharmacy and Research Institute of Drug Development, Chonnam National University)
Publication Information
Journal of Life Science / v.21, no.10, 2011 , pp. 1355-1363 More about this Journal
Abstract
Many clinical trials have demonstrated the beneficial effects of garlic (Allium sativum) on general cardiovascular health. Aged garlic extract (AGE) is known to display diverse biological activities such as in antioxidant, anti-inflammatory and anticancer activities. However, few studies have been directed on the effect of AGE on cardiovascular function. In this study, we aimed to investigate the effect of AGE and its components on platelet activation, a key contributor in thrombotic diseases. In freshly isolated rat platelets, AGE and its components have shown inhibitory activities on thrombin-induced platelet aggregation. These in vitro results were further confirmed in an in vivo platelet aggregation measurement where tail vein injection of garlic oil and S-Allylmercapto-cysteine (SAMC) significantly reduced thrombin and ADP-induced platelet aggregation. Potential active components for antiplatelet effects of AGE were identified to be SAMC and diallyl sulphide through agonist-induced platelet aggregation assay. These results indicate that aged garlic extract can be a novel dietary supplement for the prevention of cardiovascular risks and the improvement of blood circulation.
Keywords
Aged garlic extract (AGE); antiplatelet activity; diallyl sulphide; S-Allylmercapto-cysteine (SAMC);
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1 Srivastava, K. C. 1984. Aqueous extracts of onion, garlic and ginger inhibit platelet aggregation and alter arachidonic acid metabolism. Biomed. Biochim. Acta. 43, S335-346.
2 Superko, H. R. and R. M. Krauss. 2000. Garlic powder, effect on plasma lipids, postprandial lipemia, low-density lipoprotein particle size, high-density lipoprotein subclass distribution and lipoprotein(a). J. Am. Coll. Cardiol. 35, 321-326.   DOI
3 Tsao, S. M. and M. C. Yin. 2001. In-vitro antimicrobial activity of four diallyl sulphides occurring naturally in garlic and Chinese leek oils. J. Med. Microbiol. 50, 646-649.
4 Vanderhoek, J. Y., A. N. Makheja, and J. M. Bailey. 1980. Inhibition of fatty acid oxygenases by onion and garlic oils. Evidence for the mechanism by which these oils inhibit platelet aggregation. Biochem. Pharmacol. 29,3169-3173.   DOI
5 Wang, H. X. and T. B. Ng. 1999. Natural products with hypoglycemic, hypotensive, hypocholesterolemic, antiatherosclerotic and antithrombotic activities. Life Sci. 65, 2663-2677.   DOI
6 Wu, C. C., L. Y. Sheen, H. W. Chen, S. J. Tsai, and C. K. Lii. 2001. Effects of organosulfur compounds from garlic oil on the antioxidation system in rat liver and red blood cells. Food Chem. Toxicol. 39, 563-569.   DOI
7 Yamada, K., A. Naemura, N. Sawashita, Y. Noguchi, and J. Yamamoto. 2004. An onion variety has natural antithrombotic effect as assessed by thrombosis/thrombolysis models in rodents. Thromb. Res. 114, 213-220.   DOI   ScienceOn
8 Puri, R. N. 1998. Phospholipase A2: its role in ADP- and thrombin-induced platelet activation mechanisms. Int. J. Biochem. Cell Biol. 30, 1107-1122.   DOI
9 Qi, R., F. Liao, K. Inoue, Y. Yatomi, K. Sato, and Y. Ozaki. 2000. Inhibition by diallyl trisulfide, a garlic component, of intracellular Ca(2+) mobilization without affecting inositol-1,4, 5-trisphosphate (IP(3)) formation in activated platelets. Biochem. Pharmacol. 60, 1475-1483.   DOI
10 Rahman, K. and D. Billington. 2000. Dietary supplementation with aged garlic extract inhibits ADP-induced platelet aggregation in humans. J. Nutr. 130, 2662-2665.
11 Rahman, K. 2001. Historical perspective on garlic and cardiovascular disease. J. Nutr. 131, 977S-979S.
12 Rahman, K. 2003. Garlic and aging: new insights into an old remedy. Ageing Res. Rev. 2, 39-56.   DOI
13 Rahman, K. and G. M. Lowe. 2006. Garlic and cardiovascular disease: a critical review. J. Nutr. 136, 736S-740S.
14 Rahman, K. 2007. Effects of garlic on platelet biochemistry and physiology. Mol. Nutr. Food Res. 51, 1335-1344.   DOI
15 Steiner, M., A. H. Khan, D. Holbert, and R. I. Lin. 1996. A double-blind crossover study in moderately hypercholesterolemic men that compared the effect of aged garlic extract and placebo administration on blood lipids. Am. J. Clin. Nutr. 64, 866-870.
16 Gachet, C. 2001. ADP receptors of platelets and their inhibition. Thromb. Haemost. 86, 222-232.
17 Steiner, M. and R. S. Lin. 1998. Changes in platelet function and susceptibility of lipoproteins to oxidation associated with administration of aged garlic extract. J. Cardiovasc. Pharmacol. 31, 904-908.   DOI
18 Steiner, M. and W. Li. 2001. Aged garlic extract, a modulator of cardiovascular risk factors: a dose-finding study on the effects of AGE on platelet functions. J. Nutr. 131, 980S-984S.
19 Dwivedi, C., A. Abu-Ghazaleh, and J. Guenther. 1996. Effects of diallyl sulfide and diallyl disulfide on cisplatin-induced changes in glutathione and glutathione-S-transferase activity. Anticancer Drugs 7, 792-794.   DOI
20 Gachet, C. 2001. Identification, characterization, and inhibition of the platelet ADP receptors. Int. J. Hematol. 74, 375-381.   DOI
21 Gadkari, J. V. and V. D. Joshi. 1991. Effect of ingestion of raw garlic on serum cholesterol level, clotting time and fibrinolytic activity in normal subjects. J. Postgrad. Med. 37, 128-131.
22 Haber, D., M. H. Siess, M. C. Canivenc-Lavier, A. M. Le Bon, and M. Suschetet. 1995. Differential effects of dietary diallyl sulfide and diallyl disulfide on rat intestinal and hepatic drug-metabolizing enzymes. J. Toxicol. Environ. Health 44, 423-434.   DOI
23 Jackson, S. P. and S. M. Schoenwaelder. 2003. Antiplatelet therapy: in search of the 'magic bullet'. Nat. Rev. Drug Discov. 2, 775-789.   DOI
24 Lawson, L. D., D. K. Ransom, and B. G. Hughes. 1992. Inhibition of whole blood platelet-aggregation by compounds in garlic clove extracts and commercial garlic products. Thromb. Res. 65, 141-156.   DOI
25 Bordia, A. 1978. Effect of garlic on human platelet aggregation in vitro. Atherosclerosis 30, 355-360.   DOI
26 Legnani, C., M. Frascaro, G. Guazzaloca, S. Ludovici, G. Cesarano, and S. Coccheri. 1993. Effects of a dried garlic preparation on fibrinolysis and platelet aggregation in healthy subjects. Arzneimittelforschung 43, 119-122.
27 Munday, J. S., K. A. James, L. M. Fray, S. W. Kirkwood, and K. G. Thompson. 1999. Daily supplementation with aged garlic extract, but not raw garlic, protects low density lipoprotein against in vitro oxidation. Atherosclerosis 143, 399-404.   DOI
28 Block, E. 1996. Recent results in the organosulfur and organoselenium chemistry of genus Allium and Brassica plants. Relevance for cancer prevention. Adv. Exp. Med. Biol. 401, 155-169.   DOI
29 Bordia, A., S. K. Verma, and K. C. Srivastava. 1996. Effect of garlic on platelet aggregation in humans: a study in healthy subjects and patients with coronary artery disease. Prostaglandins Leukot. Essent. Fatty Acids 55, 201-205.   DOI
30 Bordia, A., S. K. Verma, and K. C. Srivastava. 1998. Effect of garlic (Allium sativum) on blood lipids, blood sugar, fibrinogen and fibrinolytic activity in patients with coronary artery disease. Prostaglandins Leukot. Essent. Fatty Acids 58, 257-263.   DOI
31 Briggs, W. H., J. D. Folts, H. E. Osman, and I. L. Goldman. 2001. Administration of raw onion inhibits platelet-mediated thrombosis in dogs. J. Nutr. 131, 2619-2622.
32 Chang, H. S, O. Yamato, Y. Sakai, M. Yamasaki, and Y. Maede. 2004. Acceleration of superoxide generation in polymorphonuclear leukocytes and inhibition of platelet aggregation by alk(en)yl thiosulfates derived from onion and garlic in dogs and humans. Prostaglandins Leukot. Essent. Fatty Acids 70, 77-83.   DOI
33 Barrett, N. E., L. Holbrook, S. Jones, W. J. Kaiser, L. A. Moraes, R. Rana, T. Sage, R. G. Stanley, K. L. Tucker, B. Wright, and J. M. Gibbins. 2008. Future innovations in anti-platelet therapies. Br. J. Pharmacol. 154, 918-939.   DOI
34 Dillon, S. A., G. M. Lowe, D. Billington, and K. Rahman. 2002. Dietary supplementation with aged garlic extract reduces plasma and urine concentrations of 8-iso-prostaglandin F(2 alpha) in smoking and nonsmoking men and women. J. Nutr. 132, 168-171.
35 Dillon, S. A., R. S. Burmi, G. M. Lowe, D. Billington, and K. Rahman. 2003. Antioxidant properties of aged garlic extract: an in vitro study incorporating human low density lipoprotein. Life Sci. 72, 1583-1594.   DOI
36 Apitz-Castro, R., S. Cabrera, M. R. Cruz, E. Ledezma, and M. K. Jain. 1983. Effects of garlic extract and of three pure components isolated from it on human platelet aggregation, arachidonate metabolism, release reaction and platelet ultrastructure. Thromb. Res. 32, 155-169.   DOI
37 Baigent, C., L. Blackwell, R. Collins, J. Emberson, J. Godwin, R. Peto, J. Buring, C. Hennekens, P. Kearney, T. Meade, C. Patrono, M. C. Roncaglioni, and A. Zanchetti. 2009. Aspirin in the primary and secondary prevention of vascular disease: collaborative meta-analysis of individual participant data from randomised trials. Lancet 373, 1849-1860.   DOI
38 Banerjee, S. K. and S. K. Maulik. 2002. Effect of garlic on cardiovascular disorders: a review. Nutr. J. 1, 4.   DOI