Browse > Article
http://dx.doi.org/10.5352/JLS.2010.20.9.1314

Construction and Analysis of Binary Vectors for Co-Overexpression, Tissue- or Development-Specific Expression and Stress-Inducible Expression in Plant  

Lee, Young-Mi (Department of Molecular Biology, Pusan National University)
Park, Hee-Yeon (Department of Molecular Biology, Pusan National University)
Woo, Dong-Hyuk (Department of Molecular Biology, Pusan National University)
Seok, Hye-Yeon (Department of Molecular Biology, Pusan National University)
Lee, Sun-Young (Ulsan University Hospital Biomedical Research Center, University of Ulsan)
Moon, Yong-Hwan (Department of Molecular Biology, Pusan National University)
Publication Information
Journal of Life Science / v.20, no.9, 2010 , pp. 1314-1323 More about this Journal
Abstract
In this study, we constructed various kinds of binary vectors with the pPZP backbone for co-overexpression, tissue- or development-specific expression and stress-inducible expression, and validated them for ectopic expression of target genes. Using a modified CaMV 35S promoter, a binary vector was generated for co-overexpression of two different genes and was confirmed to be efficient for overexpressing two different target genes at the same time and place. Binary vectors containing At2S3, KNAT1 or LFY promoters were constructed for tissue-specific or development-specific gene expression, and the binary vectors were suited for embryo/young seedling stage-, shoot apical meristem- or leaf primordia-specific expressions. Furthermore, the binary vectors containing RD29A or AtNCED3 promoters were validated as suitable vectors for gene expression induced by abiotic stresses such as high salt, ABA, MV and low temperature. Taken together, the binary vectors constructed in this study would be very useful for analyzing the biological functions of target genes and molecular mechanisms through ectopic expression.
Keywords
Binary vector; co-overexpression; tissue-specific; development-specific; stress-inducible;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Kamo, K. K. 2003. Long-term expression of the uidA gene in Gladiolus plants under control of either the ubiquitin, rolD, mannopine synthase, or cauliflower mosaic virus promoters following three seasons of dormancy. Plant Cell Rep. 21, 797-803.
2 Lee, L. Y. and S. B. Gelvin. 2008. T-DNA binary vectors and systems. Plant Physiol. 146, 325-332.   DOI
3 Lee, Y. M., H. Y. Seok, H. Y. Park, J. I. Park, J. S. Han, T. S. Bang, and Y. H. Moon. 2009. Construction and verification of useful vectors for ectopic expression and suppression of plant genes. J. Life Sci. 19, 809-817.   DOI
4 Lincoln, C., J. Long, J. Vamaguchi, K. Serikawa, and S. Hake. 1994. A knottedl-like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants. Plant Cell 6, 1859-1876.   DOI
5 Ma, B. L., K. D. Subedi, and L. M. Reid. 2004. Extent of Cross-Fertilization in Maize by Pollen from Neighboring Transgenic Hybrids. Crop Sci. 44, 1273-1282.   DOI
6 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth bioassays with tobacco tissue cultures. Plant Physiol. 15, 473-497.   DOI
7 Christensen, A. H., R. A. Sharrock, and P. H. Quail. 1992. Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol. Biol. 18, 675-689.   DOI
8 Clough, S. J. and A. F. Bent. 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735-743.   DOI
9 Deblaere, R., A. Reynaerts, H. Hofte, J. P. Hernalsteens, J. Leemans, and M. Van Montagu. 1987. Vectors for cloning in plant cells. Meth. Enzymol. 153, 277-292.   DOI
10 Gallagher, S. R. 1992. GUS protocols: using the GUS gene as a reporter of gene expression. San Diego CA, USA: Academic Press Inc.
11 Gandhi, R., S. C. Maheshwari, and P. Khurana. 1999. Transient gene expression and influence on foreign gene expression in Arabidopsis thaliana. In Vitro Cell Dev. Biol. Plant 35, 232-237.   DOI
12 Garbarino, J. E., T. Oosumi, and W. R. Belknap. 1995. Isolation of a polyubiquitin promoter and its expression in transgenic potato plants. Plant Physiol. 109, 1371-1378.   DOI
13 Guerche, P., C. Tire, F. G. De Sa, A. De Clercq, M. Van Montagu, and E. Krebbers. 1990. Differential expression of the Arabidopsis 2S Albumin genes and the effect of increasing gene family size. Plant Cell 2, 469-478.   DOI
14 Hajdukiewicz, P., Z. Svab, and P. Maliga. 1994. The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol. Biol. 25, 989-994.   DOI
15 Hauke, H., M. C. Guitton, and R. Reski. 2002. Plant functional genomics. Naturwissenschaften 89, 235-249.   DOI
16 Holtorf, S., K. Apel, and H. Bohlmann. 1995. Comparison of different constitutive and inducible promoters for the overexpression of transgenes in Arabidopsis thaliana. Plant Mol. Biol. 29, 637-646.   DOI
17 Shinozaki, K. and K. Yamaguchi-Shinozaki. 2006. Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis. Plant Mol. Biol. 60, 51-68.   DOI
18 An, G. 1986. Development of plant promoter expression vectors and their use for analysis of differential activity of nopaline synthase promoter in transformed tobacco tissue. Plant Physiol. 81, 86-91.   DOI
19 Bevan, M. 1984. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 12, 8711-8721.   DOI
20 Blazquez, M. A., C. Ferrandiz, F. Madueno, and F. Parcy. 2006. How floral meristems are built. Plant Mol. Biol. 60, 855-870.   DOI
21 Thomashow, M. F. 1999. Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50, 571-599.   DOI
22 Weigel, D. and J. Glazebrook. 2006. Transformation of Agrobacterium using the freeze-thaw method. Cold Spring Harb. Protoc. 10, 1101.
23 Norris, S. R., S. E. Meyer, and J. Callis. 1993. The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression. Plant Mol. Biol. 21, 895-906.   DOI
24 Xiong, L., H. Lee, M. Ishitani, and J. K. Zhu. 2002. Regulation of osmotic stress–`responsive gene expression by the LOS6/ABA1 locus in Arabidopsis. J. Biol. Chem. 277, 8588-8596.   DOI
25 Yamaguchi-Shinozaki, K. and K. Shinozaki. 1994. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salinity stress. Plant Cell 6, 251-264.   DOI
26 Nakashima, K., Y. Fujita, K. Katsura, K. Maruyama, Y. Narusaka, M. Seki, K. Shinozaki, and K. Yamaguchi- Shinozaki. 2006. Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis. Plant Mol. Biol. 60, 51-68.   DOI
27 Sakuma, Y., K. Maruyama, Y. Osakabe, F. Qin, M. Seki, K. Shinozaki, and K. Yamaguchi-Shinozaki. 2006. Functional Analysis of an Arabidopsis Transcription Factor, DREB2A, Involved in Drought-Responsive Gene Expression. Plant Cell 18, 1292-1309.   DOI
28 Okamuro, J. K. and R. B. Goldberg. 1989. Regulation of plant gene expression: General principles. In PK stumpf. The Biochemistry of Plants: Academic Press Inc.
29 Parcy, F., O. Nilsson, M. A. Busch, I. Lee, and D. Weigel. 1998. A genetic framework for floral patterning. Nature 395, 561-566.   DOI
30 Plesse, B., M. C. Criqui, A. Durr, Y. Parmentier, J. Fleck, and P. Genchik. 2001. Effects of the polyubiquitin gene Ubi.U4 leader intron and first ubiquitin monomer on reporter gene expression in Nicotiana tabacum. Plant Mol. Biol. 45, 655-667.   DOI
31 Sanchez, R., M. Y. Kim, M. Calonje, Y. H. Moon, and Z. R. Sung. 2009. Temporal and spatial requirement of EMF1 activity for Arabidopsis vegetative and reproductive development. Mol. Plant 2, 643-653.   DOI
32 Schultz, E. A. and G. W. Haughn. 1991. LEAFY, a homeotic gene that regulates inflorescence development in Arabidopsis. Plant Cell 3, 771-781.   DOI
33 Sheen, J. 2001. Signal Transduction in Maize and Arabidopsis Mesophyll Protoplasts. Plant Physiol. 127, 1466-1475.   DOI
34 Iuchi, S., M. Kobayashi, T. Taji, M. Naramoto, M. Seki, T. Kato, S. Tabata, Y. Kakubari, K. Yamaguchi-Shinozaki, and K. Shinozaki. 2001. Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis. Plant J. 27, 325-333.   DOI
35 Jackson, D., B. Veit, and S. Hake. 1994. Expression of maize KNOTTED-1 related homeobox genes in the shoot apical meristem predicts patterns of morphogenesis in the vegetative shoot. Development 120, 405-413.