Browse > Article
http://dx.doi.org/10.5352/JLS.2010.20.8.1214

Characteristics and Antibiotic Susceptibility of Imipenem-Resistant Clinical Isolates Producing Carbapenemase  

Choe, Han-Na (Department of Biology, Sunchon National University)
Park, Chul (Department of Biology, Sunchon National University)
Kim, Hyung-Rak (Department of Biology, Sunchon National University)
Baik, Keun-Sik (Department of Biology, Sunchon National University)
Kim, Se-Na (Department of Biology, Sunchon National University)
Seong, Chi-Nam (Department of Biology, Sunchon National University)
Publication Information
Journal of Life Science / v.20, no.8, 2010 , pp. 1214-1220 More about this Journal
Abstract
Imipenem-resistant bacteria were isolated from clinical specimens taken from hospitalized patients in Suncheon, Korea. Fifty-four isolates were phylogenetically analyzed based on 16S rRNA gene and gyrB gene sequence comparisons. Isolates were affiliated with Pseudomonas aeruginosa (30 strains; 55.6%), Acinetobacter baumannii (21; 38.9%), Enterobacter hormaechei (2) and Pseudomonas putida (2). Twenty-two isolates produced metallo-$\beta$-lactamase (MBL); 12 Acinetobacter baumannii strains, 7 Pseudomonas aeruginosa strains, 2 P. putida strains and 1 Enterobacter hormaechei strain. Antibiotic susceptibility of the isolates was determined using the disc diffusion method and Vitek system. Strains producing metallo-$\beta$-lactamase (type IMP & VIM) were more resistant to antibiotics ceftazidime, aztreonam, amikacin and gentamicin than to strains producing OXA and SHV type of $\beta$-lactamase.
Keywords
Imipenem-resistant; 16S rRNA gene; Acinetobacter baumannii; metallo-$\beta$-lactamase; antibiotic susceptibility;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Walsh, T. R., M. A. Toleman, L. Poirel, and P. Nordmann. 2005. Metallo-$\beta$-lactamases: the quiet before the storm?. Clin.Microbiol. Rev. 18, 306-325.   DOI
2 Yamamoto, S. and S. Harayama. 1995. PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl. Environ. Microbiol. 61, 1104-1109.
3 Lee, S., Y. J. Park, M. Kim, H. K. Lee, K. Han, C. S. Kang,and M. W. Kang. 2005. Prevalence of Ambler class A and D $\beta$-lactamases among clinical isolates of Pseudomonas aeruginosa in Korea. J. Antimicrob. Chemother. 56, 122-127.   DOI
4 Livingstone, D., M. J. Gill, R. Wise. 1995. Mechanism of resistance to the carbapenems. J. Antimicrob. Chemother. 35, 1-5.   DOI
5 Paton. R., R. S. Miles, J. Hood, S. G. Amyes, R. S. Miles, and S. G. Amyes. 1993. ARI 1: $\beta$-lactamase-mediated imipenem resistance in Acinetobacter baumannii. Int. J. Antimicrob. Agents. 2, 81-87.   DOI
6 Pournaras, S., A. Tsakris, M. Maniati, L. S. Tzouvelekis, and A. N. Maniatis. 2002. Novel variant (blaVIM-4) of the metallo-$\beta$-lactamase gene blaVIM-1 in a clinical strain of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 46, 4026-4028.   DOI
7 Quinn, J. P., E. J. Dudek, C. A. DiVincenzo, D. A. Lucks, and S. A. Lerner. 1986. Emergence of resistance to imipenem during therapy for Pseudomonas aeruginosa infections. J. Infect. Dis. 154, 289-294.   DOI   ScienceOn
8 Rasmussen, B. A., Y. Gluzman, and E. P. Tally. 1990. Cloning and sequencing of the class B $\beta$-lactamases gene (ccrA) from Bacteroides fragilis TAL3636. Antimicrob. Agents Chemother. 34, 1590-1592.   DOI   ScienceOn
9 Smibert, R. M. and N. R. Krieg. 1994. General characterization. In Methods for General and Molecular Bacteriology, pp. 607-654. Edited by P. Gebhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC:American Society for Microbiology.
10 Saitou, N. and M. Nei. 1987. The neighbor-joining method:a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
11 Toleman, M. A., A. M. Simm, T. A. Murphy, A. C. Gales, D. J. Biedenbach, R. N. Jones, and T. R. Walsh. 2002. Molecular characterization of SPM-1, a novel metallo-$\beta$-lactamase isolated in Latin America: report from the SENTRY antimicrobial surveillance programme. J.Antimicrob. Chemother. 50, 673-679.   DOI
12 Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783-791.   DOI
13 Jeong, S. H., K. Lee, Y. Chong, J. H. Yum, S. H. Lee, H. J. Choi, Y. M. Kim, K. H. Park, B. H. Han, S. W. Lee, and T. S. Jeong. 2003. Characterization of a new integron containing VIM-2, a metallo-$\beta$-lactamase gene cassettes, in a clinical isolate of Enterobacter cloacae. J. Antimicrob. Chemother. 51, 397-400.   DOI
14 Jukes, T. H. and C. R. Cantor. 1969. Evolution of protein molecules. In Mammalian protein metabolism, pp. 21-132. Edited by H. N. Munro. New York: Academic Press.
15 Kim, I. S., W. I. Oh, J. H. Song, and N. Y. Lee. 2004. Screening and identification of metallo-$\beta$-lactamase gene in clinical isolates of imipenem-resistant Pseudomonas aeruginosa. Korean J. Lab. Med. 24, 177-182.
16 Kovacs, N. 1956. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature (London) 178, 703.
17 Baik, K. S. 2005. Prokaryotic Diversity of Woopo Wetland Estimated by Phylogenetic Analysis. Ph. D. Thesis. Sunchon National University. Korea.
18 Lauretti, L., M. I. Ricco, A. Mazzriol, G. Cornaglia, G. Amicosante, R. Fontana, and M. Rossolini. 1999. Cloning and characterization of blaVIM, a new integron-borne metallo-$\beta$-lactamase gene form a Pseudomonas aeruginosa clinical isolate. Antimicrob. Agents Chemother. 43, 1584-1590.
19 Lee, K., W. G. Lee, Y. Uh, G. Y. Ha, J. Cho, Y. Chong, and the Korean Nationwide Surveillance of Antimicrobial Resistance Group. 2003. VIM- and IMP-type metallo-$\beta$-lactamase producing Pseudomonas spp. and Acinetobacer spp. in Korean Hospitals. Emerg. Infect. Dis. 9, 868-871.   DOI
20 Lee, K., J. H. Yum, D. Yong, H. M. Lee, H. D. Kim, J. D. Docquier, G. M. Rossolini, and Y. Chong. 2005. Novel acquired metallo-$\beta$-lactamase gene, bl a SIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea. Antimicrob. Agents Chemother. 49, 4485-4491.   DOI
21 Castanheira, M., M. A. Toleman, R. N. Jones, F. J. Schmidt, and T. R. Walsh. 2004. Molecular characterization of a $\beta$-lactamase gene, blaGIM-1, encoding a new subclass of metallo-$\beta$-lactamase. Antimicrob. Agents Chemother. 48, 4654-4661.   DOI
22 Edwards, J. R., P. J. Turner, and C. Wannop. 1989. In vitro antibacterial activity of SM-7338, a carbapenem antibiotics with stability to dehydropeptidase I. Antimicrob. Agents Chemother. 33, 215-222.   DOI   ScienceOn
23 CLSI. 2003. Performance standards for antimicrobial disk susceptibility tests, 8th edn. Approved Standard M2-A8. Wayne, PA: Clinical Laboratory Standards Institute.
24 Cohen, S. N., A. C. Y. Chang, and L. Hsu. 1972. Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R- factor DNA. Proc. Natl. Acad. Sci. 69, 2110-2114.   DOI
25 Crespo, M. P., N. Woodfood, A. Sinclair, M. E. Kaufmann, J. Turton, J. Glover, J. D. Velez, C. R. Castaneda, M. Recalde, and D. M. Livermore. 2004. Outbreak of Carbapenem-Resistant Pseudomonas aeruginosas Producing VIM-8, a Novel Metallo-$\beta$-Lactamas, in a Tertiary Care Center in Cali, Colombia. J. Clin. Microbiol. 42, 5094-5101.   DOI