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http://dx.doi.org/10.5352/JLS.2010.20.7.991

Cobalt Chloride-Induced Down-Regulation of Puromycin-Sensitive Aminopeptidase Involved in Apoptosis of PC-3 Cells  

Lee, Suk-Hee (Division of Biological Sciences, Chonbuk National University)
Kim, Hwan-Gyu (Division of Biological Sciences, Chonbuk National University)
Publication Information
Journal of Life Science / v.20, no.7, 2010 , pp. 991-998 More about this Journal
Abstract
Hypoxia is an indicative of pro-apoptotic and anti-apoptotic biphasic effects, which appear to be dependent upon the cell type and the condition of the cells. The hypoxia-mimetic agent, cobalt chloride ($CoCl_2$), has been shown to induce apoptosis in a variety of cell types, but the mechanism by which this occurs has yet to be thoroughly elucidated. Puromycin-sensitive aminopeptidase (PSA) gene was decreasingly expressed in response to $CoCl_2$. In this report, puromycin pretreatment applied to PC-3 cells resulted in apoptosis. To determine whether PSA is involved in apoptosis, we examined the apoptotic properties of the PC-3 cells after siRNA knockdown of PSA. PSA siRNA-induced PSA silencing revealed that endogenous PSA may be involved in apoptosis of the PC-3 cells. These results indicated that PSA may perform a vital function in cell survival of the PC-3 cells.
Keywords
Cobalt chloride; puromycin-sensitive aminopeptidase (PSA); apoptosis; puromycin;
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