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http://dx.doi.org/10.5352/JLS.2010.20.7.977

G1 Arrest of the Cell Cycle by Gomisin N, a Dibenzocyclooctadiene Lignan, Isolated from Schizandra chinensis Baill in Human Leukemia U937 Cells  

Park, Cheol (Blue-Bio Industry Regional Innovation Center, Dongeui University)
Hwang, Hye-Jin (Department of Food and Nutrition, College of Human Ecology, Dongeui University)
Choi, Byung-Tae (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University)
Choi, Tae-Hyun (Department of Ophthalmic Optics, Daegu Technical University)
Kim, Byung-Woo (Department of Life Science and Biotechnology, College of Natural Sciences, Dongeui University)
Choi, Young-Whan (Department of Horticultural Bioscience, Pusan National University)
Choi, Yung-Hyun (Department of Biomaterial Control, Graduate School, Dongeui University)
Publication Information
Journal of Life Science / v.20, no.7, 2010 , pp. 977-982 More about this Journal
Abstract
We investigated the anti-cancer effects of two dibenzocyclooctadiene lignans, gomisin A and gomisin N, isolated from Schizandra chinensis Baill, in human promyelocytic U937 cells. Gomisin N, but not gomisin A, inhibited cell growth in a concentration-dependent manner, which was associated with the induction of G1 arrest of the cell cycle. G1 arrest induced by gomisin N was correlated with down-regulation of cyclin E, cyclin-dependent kinase (Cdk) 2 and Cdk4, and a concomitant up-regulation of Cdk inhibitors such as p16 (INK4A) and p21 (WAF1/CIP1). Furthermore, gomisin N inhibited phosphorylation of retinoblastoma protein (pRB) and p130, and expression of transcription factor E2Fs. The results indicated that growth inhibition by gomisin N is related to cell cycle arrest at G1 in U937 cells and these findings suggest that gomisin N may be a useful chemotherapeutic agent.
Keywords
Gomisin N; U937; cell cycle; G1 arrest;
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