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http://dx.doi.org/10.5352/JLS.2010.20.7.1121

Protective Effect of Prunella spica Extracts against H2O2-Induced Cytotoxicity in PC12 Cells  

Kim, Hyun-Jung (Department of Food Science and Biotechnology, Kyungnam University)
Lee, Jeung-Min (Department of Food Science and Biotechnology, Kyungnam University)
Moon, Seong-Hee (Department of Food Science and Biotechnology, Kyungnam University)
Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
Publication Information
Journal of Life Science / v.20, no.7, 2010 , pp. 1121-1126 More about this Journal
Abstract
The oxidative stress induced by reactive oxygen species (ROS) may play an important role in the pathogenesis of neurodegenerative diseases. In this study, we investigated the neuroprotective effects of methanolic extracts of Prunella Spica (PSE) against $H_2O_2$-induced oxidative stress in PC12 cells. The cells exposed to $H_2O_2$-induced oxidative stress were treated with various concentrations of PSE; this treatment resulted in the induction of a dose-dependent protective effect, which was evidenced by the results of MTT reduction assay, lactate dehydrogenase (LDH) release assay, morphological assay, and colony-formation assay. Interestingly, we also observed reduction of apoptotic bodies in the Hoechst staining and flow cytometric analysis. These data show that apoptosis was significantly suppressed in the PC12 cells that were exposed to $H_2O_2$-induced oxidative stress and treated with PSE. These results suggest that Prunella Spica could be a new potential protective agent against $H_2O_2$-induced oxidative stress.
Keywords
Oxidative stress; neuroprotective effect; Prunella Spica; PC12 cells; apoptosis;
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