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http://dx.doi.org/10.5352/JLS.2010.20.6.964

Prevalence of Plasmid-Mediated Quinolone Resistance Genes in Escherichia coli Isolated from Diseased Animals in Korea  

Shin, Dong-Ho (National Veterinary Research and Quarantine Service)
Kim, Ha-Young (National Veterinary Research and Quarantine Service)
Byun, Jae-Won (National Veterinary Research and Quarantine Service)
Kim, Dae-Keun (National Veterinary Research and Quarantine Service)
Lim, Suk-Kyung (National Veterinary Research and Quarantine Service)
Jung, Byeong-Yeal (National Veterinary Research and Quarantine Service)
Publication Information
Journal of Life Science / v.20, no.6, 2010 , pp. 964-967 More about this Journal
Abstract
Plasmid-mediated quinolone resistance (PMQR) determinants have been contributed to quinolone resistance of gram-negative bacteria worldwide. However, little data on the prevalence of these determinants in bacteria from animals are available in Korea. In this study, the prevalence of PMQR genes was investigated with E. coli originating from diseased animals. Among 55 E. coli tested, 11 showed PMQR genes by PCR. The most prevalent genotype was qepA (14.5%), followed by aac(6')-Ib-cr (7.3%) and qnrS (1.8%). Interestingly, two isolates with PMQR genes did not show quinolone resistance in this study. The isolates exhibited higher fluoroquinolone resistance in aac(6')-Ib-cr in combination with qnrS or qepA compared with aac(6')-Ib-cr only. In a conjugal transfer test, PMQR genes were transferred from donor to recipient.
Keywords
Plasmid-mediated quinolone resistance (PMQR); qnrS; aac(6')-Ib-cr; qepA;
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