Browse > Article
http://dx.doi.org/10.5352/JLS.2010.20.5.655

Suppression of Human GD3 Synthase (hST8Sia I) Expression Induced by Retinoic Acid in Human Melanoma SK-MEL-2 Cells  

Kwon, Haw-Young (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Kang, Nam-Young (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Lee, Young-Choon (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Publication Information
Journal of Life Science / v.20, no.5, 2010 , pp. 655-661 More about this Journal
Abstract
To elucidate the mechanism underlying the suppressive regulation of hST8Sia I expression in retinoic acid (RA)-induced SK-MEL-2 cells, we characterized the promoter region of the hST8Sia I gene. Functional analysis of the 5‘-flanking region of the hST8Sia I gene by the transient expression method showed that the -1146 to -646 region, which contains putative binding sites for transcription factors c-Ets-1, CREB, AP-1 and NF-kB, functions as the RA-repressive promoter in SK-MEL-2 cells. Site-directed mutagenesis and ChIP analyses indicated that the NF-kB binding site at -731 to -722 is crucial for the RA-induced repression of hST8Sia I in SK-MEL-2 cells. In addition, the transcriptional activity of hST8Sia I suppressed by RA in SK-MEL-2 cells was strongly inhibited by extracellular signal-regulated protein kinase (ERK) inhibitor U0126 and protein kinase C (PKC) inhibitor GO6976, as determined by RT-PCR and luciferase assay of hST8Sia I promoter containing the -1146 to -646 regions. These results suggest that RA markedly modulates transcriptional regulation of hST8Sia I gene expression through the PKC/ERK signal pathway in SK-MEL-2 cells.
Keywords
Human GD3 synthase; retinoic acid; transcription factor; SK-MEL-2;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kang, N. Y., S. K Kang, Y. C. Lee, H. J. Choi, Y. S. Lee, S. Y. Cho, Y. S. Kim, J. H. Ko, and C. H. Kim. 2006. Transcriptional regulation of the human GD3 synthase gene expression in Fas-induced Jurkat T cells: a critical role of transcription factor NF-$\kappa$B in regulated expression. Glycobiology 16, 375-389.   DOI
2 Merritt, W. D., J. T. Casper, S. J. Lauer and G. H. Reaman. 1987. Expression of GD3 ganglioside in childhood T-cell lymphoblastic malignancies. Cancer Res. 47, 1724-1730.
3 Old, L. J. 1981. Cancer immunology: the search for specificity-G. H. A. Clowes Memorial lecture. Cancer Res. 41, 361-375.
4 Pan, M., S. Geng, S. Xiao, J. Ren, Y. Liu, X. Li, Z. Li, and Z. Peng. 2009. Apoptosis induced by synthetic retinoic acid CD437 on human melanoma A375 cells involves RIG-I pathway. Arch. Dermatol. Res. 301, 15-20.   DOI
5 Fukuda, M., K. Horibe, and K. Furukawa. 1998. Enhancement of in vitro and in vivo anti-tumor activity of anti-GD2 monoclonal antibody 220-51 against human neuroblastoma by granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor. Int. J. Mol. Med. 2, 471-475.
6 Hakomori, S. 1996. Tumor malignancy defined by aberrant glycosylation and sphingo (glyco) lipid metabolism. Cancer Res. 56, 5309-5318.
7 Hakomori, S. 1981.lycosphingolipids in cellular interaction, differentiation, and oncogenesis. Annu. Rev. Biochem. 50, 733-764.   DOI
8 Haraguchi, M., S. Yamashiro, A. Yamamoto, K. Furukawa, K. Takamiya, K. O. Lloyd, H. Shiku, and K. Furukawa. 1994. Isolation of GD3 synthase gene by expression cloning of GM3 $\alpha$-2,8-sialyltransferase cDNA using anti-GD2 monoclonal antibody. Proc. Natl. Acad. Sci. USA 91, 10455-10459.   DOI
9 Kang, N. Y., C. H. Kim, K. S. Kim, J. H. Ko, J. H. Lee, Y. K. Jeong, and Y. C. Lee. 2007. Expression of the human CMP-NeuAc:GM3 $\alpha$2,8 sialyltransferase (GD3 synthase) gene through the NF-$\kappa$B activation in human melanoma SK-MEL-2 cells. Biochim. Biophys. Acta 1769, 622-630.   DOI   ScienceOn
10 Baeuerle, P. A. and D. Baltimore. 1996. NF-$\kappa$B: ten years after. Cell 87, 13-20.   DOI
11 Chen, F., V. Castranova, and X. Shi. 2001. New insights into the role of nuclear factor-$\kappa$B in cell growth regulation. Am. J. Pathol. 159, 387-397.   DOI   ScienceOn
12 Cheung, N. K., U. M. Saarinen, J. E. Neely, B. Landmeier, D. Donovan, and P. F. Coccia. 1985. Monoclonal antibodies to a glycolipid antigen on human neuroblastoma cells. Cancer Res. 45, 2642-2649.
13 Dippold, W. G., K. O. Lloyd, L. T. Li, H. Ikeda, H. F. Oettgen, and L. J. Old. 1980. Cell surface antigens of human malignant melanoma: definition of six antigenic systems with mouse monoclonal antibodies. Proc. Natl. Acad. Sci. USA, 77, 6114-6118.   DOI
14 Fields, A. L., D. R. Soprano, and K. J. Soprano. 2007. Retinoids in biological control and cancer. J. Cell Biochem. 102, 886-898.   DOI
15 Sasaki, K., K. Kurata, N. Kojima, N. Kurosawa, S. Ohta, N. Hanai, S. Tsuji, and T. Nishi. 1994. Expression cloning of a GM3-specific $\alpha$-2,8-sialyltransferase (GD3 synthase). J. Biol. Chem. 269, 15950-15956.
16 Fligiel, S. E., D. R. Inman, H. S. Talwar, G. J. Fisher, J. J. Voorhees, and J. Varani. 1992. Modulation of growth in normal and malignant melanocytic cells by all-trans retinoic acid. J. Cutan Pathol. 19, 27-33.   DOI
17 Zhang, H., S. Zhang, N.-K. V. Cheung, G. Ragupathi, and P. O. Livingston. 1998. Antibodies against GD2 ganglioside can eradicate syngeneic cancer micrometastases. Cancer Res. 58, 2844-2849.
18 Welte, K., G. Miller, P. B. Chapman, H. Yuasa, E. Natoli, J. E. Kunicka, C. Cordon-Cardo, C. Buhrer, L. J. Old, and A. N. Houghton. 1987. Stimulation of T lymphocyte proliferation by monoclonal antibodies against GD3 ganglioside. J. Immunol. 139, 1763-1771.
19 Yamaguchi, H., K. Furukawa, S. R. Fortunato, P. O. Livingston, K. O. Lloyd, H. F. Oettgen, and L. J. Old. 1987. Cell-surface antigens of melanoma recognized by human monoclonal antibodies. Proc. Natl. Acad. Sci. USA 84, 2416-2420.   DOI
20 Yamashiro, S., M. Okada, M. Haraguchi, K. Furukawa, K. O. Lloyd, H. Shiku, and K. Furukawa. 1995. Expression of $\alpha$2,8-sialyltransferase (GD3 synthase) gene in human cancer cell lines: high level expression in melanomas and up-regulation in activated T lymphocytes. Glycoconj. J. 12, 894-900.   DOI
21 Sporn, M. B. and A. B. Roberts. 1983. Role of retinoids in differentiation and carcinogenesis, Cancer Res. 43, 3034-3040.
22 Svennerholm, L. 1980. Gangliosides and synaptic transmission. Adv. Exp. Med. Biol. 125, 533-544.   DOI
23 Thampoe, I. J., K. Furukawa, E. Vellvé, and K. O. Lloyd. 1989. Sialyltransferase levels and ganglioside expression in melanoma and other cultured human cancer cells. Cancer Res. 49, 6258-6264.
24 Rusan, S., B. K. Raj, and K. O. Lloyd. 1999. Relationship of glycosyltransferases and mRNA levels to ganglioside expression in neuroblastoma and melanoma cells. J. Neurochem. 72, 514-521.   DOI
25 Portoukalian, J., G. Zwingelstein, and J. F. Dore. 1979. Lipid composition of human malignant melanoma tumors at various levels of malignant growth. Eur. J. Biochem. 94, 19-23.   DOI
26 Pukel, C. S., K. O. Lloyd, L. R. Travassos, W. G. Dippold, H. F. Oettgen, and L. J. Old. 1982. GD3, a prominent ganglioside of human melanoma. Detection and characterisation by mouse monoclonal antibody. J. Exp. Med. 155, 1133-1147.   DOI