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http://dx.doi.org/10.5352/JLS.2010.20.4.555

Pre-Exercise Protective Effects Against Renal Ischemic Reperfusion Injury in Hsp 70.1 Knockout Mice  

Lee, Jin (Department of Anatomy and Cell Biology, College of Medicine, Hanyang University)
Kim, Won-Kyu (Department of Anatomy and Cell Biology, College of Medicine, Hanyang University)
Publication Information
Journal of Life Science / v.20, no.4, 2010 , pp. 555-560 More about this Journal
Abstract
The objective of this study was to investigate levels of serum creatinine, CuSOD and MnSOD protein expression in the kidney after renal ischemic reperfusion with pre-exercise using heat shock protein 70.1 in knock-out mice (KO). The C57/BL6 strain (Wild type: WT) and KO were divided into 4 groups as follows: Sham control group (Sham), pre-exercise group (Ex), pre-exercise +ischemia group (Ex+IR), and ischemia group (IR). CuSOD and MnSOD expression were significantly decreased (p<0.01, p<0.05) and blood creatinine concentration was significantly increased (p<0.01) in the IR group of KO. In contrast, CuSOD and MnSOD expression in the Ex+IR group of KO were higher than the IR group, while creatinine concentration was significantly lower. These results suggest that Hsp70 is directly correlated to renal ischemic reperfusion injury. Pre-exercise in renal ischemia might prevent or inhibit positive oxidative stress inhibitory effects by increasing anti-oxidative enzymes (CuSOD, MnSOD) within the kidney and improve to prevent renal function. Thus, pre-exercise may have a protective role against renal injury after renal ischemia.
Keywords
Hsp70.1knock-out mouse; ischemia renal injury; pre-exercise; creatinine; SOD;
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