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http://dx.doi.org/10.5352/JLS.2010.20.3.388

Inhibition of Cyclooxygenase-2 Activity and Prostaglandin E2 Production through Down-regulation of NF-κB Activity by the Extracts of Fermented Beans  

Lee, Hye-Hyeon (Department of Medical Bioscience, Graduate School and Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Park, Cheol (Blue-Bio Industry Regional Innovation Center, Dongeui University)
Kim, Min-Jeong (Department of Medical Bioscience, Graduate School and Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Seo, Min-Jeong (Department of Medical Bioscience, Graduate School and Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Choi, Sung-Hyun (Department of Elevator Maintenance, Korea Lift College)
Jeong, Yong-Kee (Department of Medical Bioscience, Graduate School and Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Choi, Yung-Hyun (Blue-Bio Industry Regional Innovation Center, Dongeui University)
Publication Information
Journal of Life Science / v.20, no.3, 2010 , pp. 388-395 More about this Journal
Abstract
Cyclooxygenase (COX)-2 is generally known as an inducible enzyme, and it produces arachidonic acid to prostaglandin $E_2$ ($PGE_2$), which has been demonstrated to play a critical role in inflammation. In the present study, we investigated the effects of the extracts of fermented beans including soybean (FS), black agabean (FBA) and yellow agabean (FYA), on the expression of COXs and production of $PGE_2$ in U937 human promonocytic cells. Treatment of phorbol 12-myristate 13-acetate (PMA) significantly induced pro-inflammatory mediators such as COX-2 expression and $PGE_2$ production, whereas the levels of COX-1 remained unchanged. However, pre-treatment with FS, FBA and FYA significantly decreased PMA-induced COX-2 protein as well as mRNA, which is associated with inhibition of $PGE_2$ production. Moreover, FS, FBA and FYA markedly prevented the increase of nuclear translocation of nuclear factor kappa B (NF-${\kappa}B$) p65 by PMA. Our data indicate that the extracts of fermented beans exhibits anti-inflammatory properties by suppressing the transcription of pro-inflammatory cytokine genes through the NF-${\kappa}B$ signaling pathway.
Keywords
Fermented beans; COX-2; $PGE_2$; NF-${\kappa}B$;
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