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http://dx.doi.org/10.5352/JLS.2010.20.3.374

Purification and Identification of Inhibitory Compounds on Helicobacter pylori from Cheongmoknosang Callus for Biomass  

Yun, Dong-Hyuck (Department of Food Engineering Kyungpook National University)
Cha, Won-Seup (Department of Food Engineering Kyungpook National University)
Lee, Seon-Hon (The Institute of Marine biotechnology, Andong National University)
An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Hanny University)
Kim, Jeung-Hoan (NIP Biotech.)
Chun, Sung-Sook (NIP Biotech.)
Bae, Jong-Ho (Department of Confectionery Decoration, Daegu Mirae College)
Cho, Young-Je (Department of Food Engineering Kyungpook National University)
Publication Information
Journal of Life Science / v.20, no.3, 2010 , pp. 374-380 More about this Journal
Abstract
The objective of this research was to evaluate the inhibitory activities of phenolic compounds isolated from Cheomoknosang callus on Helicobacter pylori. Total phenolic compounds of 80% ethanol extracts from callus were 15.3 mg/g. The activity of H. pylori inhibition at 80% ethanol extracts from Cheongmoknosang callus was determined as 14 mm clear zone. Isolation of inhibitory compounds was carried out on Sephadex LH-20 and MCI-gel CHP-20 column chromatography using a gradient elution procedure of increasing MeOH in $H_2O$. The chemical structure of the inhibitory compound against Helicobacter pylori was confirmed as protocatechuic acid, chlorogenic acid, caffeic acid and rosemarinic acid by spectroscopic analysis of FAB-MS, NMR and IR spectrum.
Keywords
Cheongmoknosang callus; Helicobacter pylori; purification; identification;
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