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http://dx.doi.org/10.5352/JLS.2010.20.2.253

Neuroprotective Effects of Methanolic Extracts from Peanut Sprouts  

Kim, Hyun-Jung (Department of Food Science and Biotechnology, Kyungnam University)
Kang, Jum-Soon (Department of Horticultural Bioscience, Pusan National University)
Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
Hwang, Yong-Il (Department of Food Science and Biotechnology, Kyungnam University)
Publication Information
Journal of Life Science / v.20, no.2, 2010 , pp. 253-259 More about this Journal
Abstract
The neuroprotective effects of extracts from various parts of peanut sprouts on glutamate-induced neurotoxicity in N18-RE-105 cells were investigated. This study was performed to evaluate the neuroprotective activity of methanolic extracts from the whole (WME), heads (HME), and stems (SME) of peanut sprouts. The neuroprotective effects of these extracts were measured by MTT reduction assay, LDH release assay, phase-contrast microscopy, and flow cytometric analysis on the N18-RE-105 cells. Among these extracts, the HME showed the greatest neuroprotective effects, and was further fractionated with hexane, diethyl ether, ethyl acetate, and water, according to degree of polarity. Out of the fractionated extracts, the diethyl ether layer showed the highest activity on glutamate-induced cytotoxicity in N18-RE-105 cells. The sub-G1 DNA contents of the glutamate-induced severely apoptotic N18-RE-105s were measured by flow cytometric analysis to confirm the HME's anti-apoptotic activity. Interestingly, after incubation with 100 mg/ml of the HME, the proportion of sub-G1 cells of the glutamate-stressed N18-RE-105s had been greatly reduced, from 58.5% to 9.1%. These results imply that HME may have strong potential as a chemotherapeutic agent against neuronal diseases.
Keywords
Neuroprotective; N18-RE-105 cells; peanut sprout; apoptosis;
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1 Shin, A. Y., H. Erb, X. Sun, S. Toda, P. W. Kalivas, and T. H. Murphy. 2006. Cystine/glutamate exchange modulated glutathione supply for neuroprotection from oxidative stress and cell proliferation. J. Neurosci. 26, 10514-10523.   DOI
2 Tan, S., Y. Sagara, Y. Liu, P. Maher, and D. Schubert. 1998. The regulation of reactive oxygen species production during programmed cell death. J. Cell Biol. 141, 1423-1432.   DOI
3 Wang, F., F. Gao, M. Lan, H. Yuan, Y. Huang, and J. Liu. 2009. Oxidative stress contributes to silica nanoparticleinduced cytotoxicity in human embryonic kidney cells. Toxicol. In Vitro. 22, 808-815.
4 Yoon, M. Y., J. Y. Kim, J. H. Hwang, M. R. Cha, K. J. Jo, and H. R. Park. 2007. Protective effect of methanolic extracts from Dendrobium nobile Lindl. on $H_{2}O_{2}$-induced neurotoxicity in PC12 cells. J. Korean Soc. Appl. Chem. 50, 63-67.   과학기술학회마을
5 Yoon, M. Y., H. J. Lee, B. B. Lee, S. M. Lee, J. Y. Kim, Y. Kim, E. Park, and H. R. Rark. 2007. Protective effect of schizonepeta tenuifolia briquet extracts on oxidative DNA damage in human leucocytes and on hydrogen peroxide-induced cytotoxicity in PC12 cells. Food Sci. Biotechnol. 16, 858-862.   과학기술학회마을
6 Yoshimune, K., Y. Shirakihara, M. Wakayama, and I. Yumoto. 2009. Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator tris. FEBS J. 277, 738-748.   DOI
7 Lim, C. S., D. Q. Jin, J. Y. Sung, J. H. Lee, H. G. Choi, I. Ha, and J. S. Han. 2006. Antioxidant and anti-inflammatory activities of the methanolic extract of Neorhodomela aculeate in hippocampal and microglial cells. Biol. Pharm. Bull. 29, 1212-1216.   DOI
8 Nikolova, S., Y. S. Lee, Y. S. Lee, and J. A. Kim. 2005. Racl-NADPH oxidase-regulated generation of reactive oxygen species mediates glutamate-induced apoptosis in SH-SY5Y human neuroblastoma cells. Free Radical Res. 39, 1295-1304.   DOI
9 Marambaud, P., H. Zhao, and P. Davies. 2005. Resveratrol promotes clearance of Alzheimer's disease amyloid-$\beta$ peptides. J. Biol. Chem. 280, 37377-37382.   DOI
10 Milatovic, D., S. Zaja-Milatovic, R. C. Gupta, Y. Yu, and M. Aschmer. 2009. Oxidative damage and neurodegeneration in manganese-induced neurotoxicity. Toxicol. Appl. Pharm. 240, 219-225.   DOI
11 Murphy, T. H., M. Miyamoto, A. Sastre, R. L. Schnaar, and T. Coyle. 1989. Glutamate toxicity in a neuronal cell line involves inhibition of cystine transport leading to oxidative stress. Neuron 2, 1547-1558.   DOI
12 Penugonda, S., S. Mare, P. Lutz, W. A. Banks, and N. Ercal. 2006. Potentiation of lead-induced cell death in PC12 cells by glutamate: Protection by N-acetylcysteine amide (NACA), a novel thiol antioxidant. Toxicol. Appl. Pharm. 216, 197-205.   DOI   ScienceOn
13 Parfenova, H., S. Basuroy, S. Bhattacharya, D. Tcheranova, Y. Qu, R. F. Regan, and C. W. Leffler. 2006. Glutamate induces oxidative stress and apoptosis in cerebral vascular endothelial cells: contributions of HO-1 and HO-2 to cytoprotection. Am. J. Physiol. Cell Physiol. 290, 1399-1410.   DOI
14 Jeong, E. J., S. H. Sung, J. Kim, S. H. Kim, and Y. C. Kim. 2008. Rhus verniciflua stokes attenuates glutamate-induced neurotoxicity in primary cultures of rat cortical cells. Nat. Prod. Sci. 14, 156-160.
15 Sagara, Y., R. Dargusch, D. Chambers, J. Davis, D. Schubert, and P. Mater. 1998. Cellular mechanisms of resistance to chronic oxidative stress. Free Radic. Biol. Med. 24, 1375-1389.   DOI
16 Jeon, H. J., S. W. Park, I. Lee, and B. S. Mun. 2004. Effects of Gwibitang on glutamate-induced death in rat neonatal astrocytes. J. Korean Oriental Med. 25, 184-193.   과학기술학회마을
17 Jeong, G. S., D. S. Lee, T. O. Kwon, H. S. Lee, R. B. An, and Y. C. Kim. 2009. Cytoprotective constituents of the heartwood of Caesalpinia sappan on glutamate-induced oxidative damage in HT22 cells. Biol. Pharm. Bull. 32, 945-949.   DOI   ScienceOn
18 Kadomura, K., S. Maruse, S. Sugihara, K. Yamaguchi, and T. Oda. 2007. Production of reactive oxygen species (ROS) by various marine fish species during the larval stage. Biosci. Biotechmol. Biochem. 71, 2029-2033.   DOI
19 Kim, B. M., Y. J. Choi, Y. Han, Y. S. Yun, and S. H. Hong. 2009. N,N-dimethyl phytosphingosine induced caspase-8-dependent cytochrome c release and apoptosis through ROS generation in human leukemia cells. Toxicol. Appl. Pharm. 239, 87-97.   DOI
20 Kim, M. H., S. Uehara, A. Muroyama, B. Hille, Y. Moriyama, and D. S. Koh. 2008. Glutamate transportermediated glutamate secretion in the mammalian pineal gland. J. Neurosci. 28, 10852-10863.   DOI
21 Kim, S. H., D. K. Shin, J. Y. Choe, J. Lee, E. J. Suh, and H. S. Suh. 1999. Quantitation of the Early apoptotic cells using flow cytometry. Korean J. Chlin. Pathol. 19, 108-113.
22 Emerit, J., M. Edeas, and F. Bricaire. 2004. Neurodegenerative diseases and oxidative stress. Biomed. Pharmacother. 58, 39-46.   DOI
23 Kim, W. J., J. Y. Park, Y. K. Park, H. S. Chung, K. C. Lee, and H. K. Lee. 1997. Effects of calcium, Mugnesium, and calcium chelating agent on recovery from hyproxia in hippocampal tissue slices. J. Korean Neurosurg. Soc. 26, 491-498.
24 Cho, C. H., S. K. Kim, G. Yoo, M. H. Son, K. Park, B. L. Lim, D. C. Kim, and H. J. Chae. 2008. Resveratrol extraction from grape fruit stem and its antioxidant activity. J. Korean Soc. Appl. Bil. Chem. 51, 11-16.   과학기술학회마을
25 Choi, B. H., E. M. Hur, J. H. Lee, D. J. Jun, and K. T. Kim. 2005. Protein kinase Cd-mediated proteasomal degradation of MAP kinase phophatase-1 contributes to glutamate-induced neuronal cell death. J. Cell. Sci. 119, 1329-1340.   DOI
26 Federico, H., M. Vanesa, G. S. Guillermo, R. B. Jezabel, A. Isaac, and R. Carmen. 2007. Melatonin prevents glutamate-induced oxytosis in the HT22 mouse hippocampal cell line through an antioxidant effect specifically targeting mitochondria. J. Neurochem. 100, 736-746.   DOI
27 Ha, J. S., C. S. Lee, J. S. Maeng, K. S. Kwon, and S. S. Park. 2009. Chronic glutamate toxicity in mouse cortical neuron culture. Brain Res. 1273, 138-143.   DOI
28 Hwang, Y. I., I. C. Shin, Y. S. Song, M. J. Seung, H. J. Park, Y. M. Lee, C. B. Park, M. K. Lee, K. W. Oh, Y. Y. Shim, and J. T. Hong. 2002. Intracellular calcium concentration in the glutamate-induced cytotoxicity in PC12 cell. J. Toxicol. Pub. Health 18, 355-362.
29 Ha, J. S. and S. S. Park. 2006. Glutamate-induced oxidative stress, but not cell death, is largely dependent upon extracellular calcium in mouse neuronal HT22 cells. Neurosci. lett. 393, 165-169.   DOI