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http://dx.doi.org/10.5352/JLS.2010.20.1.119

Analysis of Genetic Variation of Perilla frutescens var. crispa Germplasm Using RAPD  

Kim, Hyeun-Kyeung (Bioresources Development Institute, Pusan National University)
Cho, Young-Son (Department of Crop Science & Biotechnology, Jinju National University)
Yang, Jae-Wan (Department of Horticulture Bioscience, College of Natural Resource & Life Science, Pusan National University)
Choi, Young-Whan (Department of Horticulture Bioscience, College of Natural Resource & Life Science, Pusan National University)
Kang, Jun-Soon (Department of Horticulture Bioscience, College of Natural Resource & Life Science, Pusan National University)
Lee, Yong-Jae (Department of Horticulture Bioscience, College of Natural Resource & Life Science, Pusan National University)
Son, Beung-Gu (Department of Horticulture Bioscience, College of Natural Resource & Life Science, Pusan National University)
Publication Information
Journal of Life Science / v.20, no.1, 2010 , pp. 119-123 More about this Journal
Abstract
Genetic variations of Chajogi (Perilla frutescens var. crispa) germplasms were investigated by using RAPD markers. Twenty-two Perilla frutescens var. crispa lines collected from various locations were subjected to RAPD analysis using 80 primers. Among them, only 22 primers showed polymorphic bands and these 22 primers provided a total of 224 bands consisting of 127 polymorphic and 97 monomorphioc bands. The polymorphic bands were subjected to phylogenetic analysis using the UPGMA method. From UPGMA, similarity co-efficiency of 22 Chajogi lines ranged from 0.72 to 0.94. The dendrogram of 22 lines obtained through the UPGMA method resulted in two groups (one major group and one minor group). Although the two groups were roughly consistent with growth phenotypes (period of flowering, period of maturity, stem length, number of branches, number of nodes, number of flower clusters and number of ovaries) in detail, much inconsistency also was present
Keywords
Perilla frutescens; DNA marker; RAPD; genetic relationship; polymorphism;
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