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http://dx.doi.org/10.5352/JLS.2010.20.11.1617

Studies of Inhibitory Mechanism on Melanogenesis by Partially Purified Asiasari radix in α-MSH Stimulated B16F10 Melanoma Cells  

Jang, Ji-Yeon (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University)
Kim, Ha-Neui (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University)
Kim, Yu-Ri (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University)
Kim, Byung-Woo (Department of Life Science and Biotechnology, College of Natural Sciences, Dongeui University)
Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University)
Choi, Byung-Tae (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University)
Publication Information
Journal of Life Science / v.20, no.11, 2010 , pp. 1617-1624 More about this Journal
Abstract
Recently, it has been found that Asiasari radix showed a hypopigmenting effect on melanogenesis through activation of mitogen-activated protein kinase (MEK)/extracellular signal-activated kinase (ERK) in B16F10 melanoma cells. However, the hypopigmenting effect of A. radix on the $\alpha$-melanocyte stimulating hormone ($\alpha$-MSH)-stimulated melanogenesis has remained unknown. The purpose of this study was to investigate the inhibitory mechanism of the partially purified A. radix (PPAR)-induced hypopigmentating effects on $\alpha$-MSH-stimulated melanogenesis in B16F10 mouse melanoma cells. PPAR strongly inhibited tyrosinase activity and leads to decreased melanin synthesis in $\alpha$-MSH-stimulated B16F10 melanoma cells. PPAR also decreased the $\alpha$-MSH-induced over-expression of the melanogenic enzymes, tyrosinase, tyrosinase-related protein (TRP)-1, dopachrome tautomerase (Dct) and microphthalmia-associated transcription factor (MITF). We further showed that PPAR inhibits $\alpha$-MSH-induced melanogenesis via phosphorylation of MEK/ERK and PI3K/Akt, and that their activation was blocked by MEK inhibitors, PD98059 and PI3K inhibitors, LY294002 in $\alpha$-MSH-stimulated B16F10 melanoma cells. These results suggest that PPAR inhibits $\alpha$-MSH-induced melanogenesis by activation of MEK/ERK and PI3K/Akt through MITF degradation, which may lead to down-regulation of tyrosinase.
Keywords
Asiasari Radix; $\alpha$-melanocyte stimulating hormone ($\alpha$-MSH); microphthalmia-associated transcription factor (MITF); mitogen-activated protein kinase (MEK)/extracellular signal-activated kinase (ERK); PI3K/Akt;
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