[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5352/JLS.2010.20.11.1569

Protective Effect of White-Skinned Sweet Potato (Ipomoea batatas L.) from Indonesia on Streptozotocin-Induced Oxidative Stress in Rats

Bachri, Moch. Saiful (Faculty of Pharmacy, Ahmad Dahlan University)
Jang, Hye-Won (Division of Endocrinology & Metabolism, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Choi, Jong-Won (College of Pharmacy, Kyungsung University)
Park, Jong-Ok (Department of Chemistry, Kyungsung University)

Publication Information

Journal of Life Science / v.20, no.11, 2010 , pp. 1569-1576 More about this Journal

Abstract

Sweet potato (Ipomoea batatas L.) is widely used in Indonesia and other countries as a traditional medicine for the treatment of diabetes mellitus (DM). The MeOH extract of white skinned sweet potatoes (WSSP) was administered orally in doses of 100 and 200 mg/kg body weight in streptozotocin (STZ)-induced diabetic rats. Experimental diabetes was induced by a single dose of STZ (45 mg/kg, i.p.) injection. Oxidative stress was measured by tissue lipid peroxide (LPO) levels, serum aspartate transaminase (AST), alanine transaminase (ALT), total triglyceride (TG), total cholesterol (TC) and by antioxidative enzymatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase in the liver. An increase in blood glucose, LPO level, AST, ALT, TG and TC levels was observed in the STZ-induced diabetic rats. Administration of MeOH extract of WSSP at a dose of 200 mg/kg for two weeks caused a significant reduction in blood glucose, LPO levels, AST, ALT, TG and TC levels in the STZ-induced diabetic rats. Furthermore, oral administration of MeOH extract showed significant improvement in the activities of antioxidant enzymes (SOD, GPx, and CAT) compared to STZ-induced diabetic rats. In conclusion, the obtained results clearly indicate the role of oxidative stress in the induction of diabetes, and that the protective effects of MeOH extracts of WSSP could be used to benefit diabetic patients.

Keywords

Oxidative stress; white-skinned sweet potato; diabetes mellitus; streptozotocin;

Citations & Related Records

Reference

1	Reitman, S. and S. Frankel. 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28, 56-63.
2	Rhoad, G. G., C. L. Gulbrandse, and A. Kagen. 1976. Serum lipoprotein and coronary artery disease in a population study of Hawaiian Japanese men. New England J. Medicine 294, 293-298. DOI
3	Richmond, W. 1976. Use of cholesterol oxidase for assay of total and free cholesterol in serum by continuous flow analysis. Clin. Chem. 22, 1579.
4	Goodman, L. S. and A. Gilman. 1985. The Pharmacological basis of therapeutics. pp. 1490-1510, 7th eds., Macmillan, New York.
5	Grover, J. K., S. Yadav, and V. Vats. 2002. Medicinal plants of India with anti-diabetic potential. Journal of Ethnopharmacology 81, 1-100. DOI
6	Habig, W., M. J. Pabst, and W. B. Jakoby. 1974. Glutathione S-transferases the first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130-7139.
7	Hauggard, N. 1968. Cellular mechanism of oxygen toxicity. Physiol. Rev. 48, 311-373.
8	Hiramatsu, K. and S. Aomori. 1988. Increased superoxide production by mononuclear cells of patients with hypertriglyceridemia and diabetes. Diabetes 37, 832-837. DOI
9	Huang, G. J., H. Y. Chang, H. J. Chen, T. L. Lu, Y. S. Chang, M. J. Sheu, and Y. H. Lin. 2008. Effects of trypsin inhibitor on plasma antioxidant activity and lipid levels in mice from sweet potato roots. J. Sci. Food Agric. 88, 2556-2562. DOI
10	Huang, Y. C., Y. H. Chang, and Y. Y. Shao. 2005. Effects of genotype and treatment on the antioxidant activity of sweet potato in Taiwan. Food Chemistry 98, 529-538.
11	Kakkar, R., J. Karla, S. V. Manth, and K. Parsad. 1995. Lipid peroxidation and activity of antioxidant enzymes in diabetic rats. Mol. Cell Biochem. 151, 113-119. DOI
12	Kano, M., T. Takayanagi, K. Harada, K. Makino, and F. Ishikawa. 2005. Antioxidant activity of anthocyanins from purple sweet potato, Ipomoea batatas cultivar Ayamurasaki. Biosci. Biotechnol. Biochem. 69, 979-988. DOI
13	Laakso, M. 2001. Insulin resistance and its impact on the approach to therapy of type 2 diabetes. Int. J. Clin. Pract. Supplemen 8-12.
14	Aebi, H. 1974. Catalase, pp. 673-684, In Bergmeyer, H. U.(ed.), Method of Enzymatic Analysis. 2, Academic press, New York.
15	Attele, A. S., Y. P. Zhou, J. T. Xie, J. A. Wu, L. Zhang, L. Dey, W. Pugh, P. A. Rue, K. S. Polonsky, and C. S. Yuan. 2002. Antidiabetic effects of Panax ginseng berry extract and the identification of an effective component. Diabetes 51, 1851-1858. DOI
16	Baynes, J. W. and S. R. Thorpe. 1997. The role of oxidative stress in diabetic complications. Curr. Opin. Endocrinol. 3, 277-284.
17	Baynes, J. W. 1991. Role of oxidative stress in the development of complications in diabetes. Diabetes 40, 405-412. DOI
18	Bernhard, L., W. Werner, P. Rudolf, K. W. Alexandra, and P. Giovanni. 2003. Mode of action of Ipomoea Batatas (Caiapo) in type 2 diabetic patients. Metabolism Clinical and Experimental 52, 875-880. DOI
19	Chaudhry, J., N. N. Ghoxh, K. Roy, and R. Chandra. 2007. Antihyperglycemic effect of a new thiazolidine analogue and its role in ameliorating oxidative stress in alloxan-induced diabetic rats. Life Sci. 80, 1135-1142. DOI
20	Craik, F. I. M. and T. A. Salthouse. 1992. Handbook of Ageing and Cognition, pp. 51-110, Hillsdale, New Jersey.
21	FAO, 2006. http://www.fao.org, cited: 15/10/2006.
22	Garber, A. J. 2002. Attenuating CV risk factors in patients with diabetes: clinical evidence to clinical practice. Diabetes, Obesity and Metabolism 4, S5-S12. DOI
23	Uchida, T., R. Tsuchiya, N. Harada, T. Tsunoda, T. Yamaguchi, T. Eto, and M. Furukawa. 1988. Ischemic changes in the pancreas of Watanabe heritable hyper-lipidemic (WHHL) rabbits. Int. J. Pancreato. 3, 261-271.
24	Temme, E. H., H. P. G. Van, E. G. Schouten, and H. Kesteloot. 2002. Effect of a plant sterol-enriched spread on serum lipids and lipoprotein in mildly hypercholesterolaemic subjects. Acta Cardiology 57, 111-115. DOI
25	Teow, C. C., V. D. Truong, R. F. McFeeters, R. L. Thompson, K. V. Pecota, and G. C. Yencho. 2007. Antioxidant activities, phenolic and ${\beta}$ -carotene contents of sweet potato genotypes with varying flesh colours. Food Chemistry 103, 829-838. DOI
26	Toshiro, M., E. Sumi, K. Mio, F. Keiichi, S. Koichi, T. Norihiko, and M. Kiyoshi. 2002. Anti-hyperglycemic effect of diacylated anthocyanin derived from Ipomoea batatas Cultivar Ayamurasaki can be achieved through the ${\alpha}$ -glucosidase inhibitory action. J. Agric. Food Chem. 5, 7244-7248.
27	Ugochukwu, N. H., N. D. Bagayoko, and M. E. Antwi. 2004. The effects of dietary caloric restriction on antioxidant status and lipid peroxidation in mild and severe streptozotocin-induced diabetic rats. Clin. Chim. Acta. 348, 121-129. DOI
28	Zhang, Z. F., S. H. Fan, Y. L. Zheng, J. Lu, D. M. Wu, Q. Shan, and B. Hu. 2009. Purple sweet potato color attenuates oxidative stress and inflammatory response induced by D-galactose in mouse liver. Food and Toxicology 47, 496-501. DOI
29	Wolff, S. P., Z. Y. Jang, and V. J. Hunt. 1991. Protein glycation and oxidative stress in diabetes mellitus and ageing. Free Radic. Biol. Med. 10, 339-352. DOI
30	Yamagishi, N., K. Nakayama, T. Wakatsuki, and T. Hatayama. 2001. Characteristic changes of stress protein expression in streptozotocin induced diabetic rats. Life Sci. 9, 2603-2609.
31	Robertson, R. P., J. Harmon, P. P. Tran, Y. Tanaka, and H. Takahashi. 2003. Glucose toxicity in ${\beta}$ -cells: type 2 diabetes, good radicals gone bad, and the glutathione connection. Diabetes 52, 581-587. DOI
32	Rully, M. 1988. Pengaruh infus batang ubi jalar (Ipomoea batatas Poir) sebagai antidiabetik pada binatang percobaan tikus. JF FMIPA UNHAS 109.
33	Ruzaid, A., I. Amin, A. G. Nawalyah, M. Hamid, and H. A. Faizul. 2005. The effect of Malaysian cocoa extract on glucose levels and lipid profiles in diabetic rats. J. Ethnopharmacol. 98, 55-60. DOI ScienceOn
34	Shuichi, K., A. Hiroyuki, and T. Hirohide. 2001. Isolation of antidiabetic components from white-skinned sweet potato (Ipomoea batatas L.). Biosci. Biotechnol. Biochem. 65, 109-114. DOI
35	Singh, N., V. Kamath, and P. S. Rajini. 2005. Attenuation of hyperglycemia and associated biochemical parameters in STZ-induced diabetic rats by dietary supplementation of potato peel powder. Clin. Chim. Acta. 353, 165-175. DOI
36	Stefek, M., N. Tribulova, A. Gajdoski, and A. Gajdosikova. 2002. The pyridoindole antioxidant stobadine attenuates histochemical changes in kidney of STZ-induced diabetic rats. Acta Histochem. 104, 413-417. DOI
37	Marklund, S. and G. Marklund. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 469 DOI
38	Szkudelski, T. 2001. The mechanism of alloxan and streptozotocin action in ${\beta}$ -cells of the rat pancreas. Physiol. Res. 50, 537-546.
39	Tarique, A., M. Sharma, K. K. Pillai, S. E. Haquea, M. M. Alam, and M. S. Zaman. 2007. Protective effect of bezafibrate on streptozotocin-induced oxidative stress and toxicity in rats. Toxicology 229, 165-172. DOI
40	Taskinen, M. R. 1993. Lipoprotein and apoproteins in diabetes. pp. 122-134, In Belfiore, F., R. N. Bergnan, and G. M. Molinatt (eds.), Current Topics in Diabetes Research 12. Informa Health Care.
41	McGowan, M. W., J. D. Artiss, and D. R. Stradbergh. 1983. A peroxidase coupled method for the colorimetric determination of serum triglycerides. Clin. Chem. 29, 583.
42	Ohkawa, H., N. Ohishi, and K. Yagi. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochem. 95, 351-358. DOI
43	Oki, T., M. Masuda, S. Furuta, Y. Nishiba, N. Terahara, and I. Suda. 2002. Involvement of anthocyanins and other phenolic compounds in radical scavenging activity of purple-fleshed sweet potato cultivars. J. Food Sci. 67, 1752-1756. DOI
44	Paglia, E. D. and W. N. Valentine. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 70, 158-169.
45	Randle, P. J., P. B. Gailand, C. N. Hales, and E. A. Neiosholine. 1963. The glucose and fatty acid cycle: its role in insulin sensitivity and metabolic disturbance of diabetes. The Lancet 1, 785-790.