Browse > Article
http://dx.doi.org/10.5352/JLS.2010.20.10.1563

Increase in the Chlorophyll Contents by Over-expression of GmNAP1 Gene in Arabidopsis Plant  

Park, Phun-Bum (Department of Bioscience and Biotechnology, University of Suwon)
Ahn, Chul-Hyun (Department of Bioscience and Biotechnology, University of Suwon)
Publication Information
Journal of Life Science / v.20, no.10, 2010 , pp. 1563-1568 More about this Journal
Abstract
In the course of a research concerning the molecular mechanism of hypocotyl elongation that occurs during soybean seedling growth in darkness, we have generated a number of ESTs from a cDNA library prepared from the hypocotyls of dark-grown soybean seedlings. Comparison of the ESTs assigned a cDNA clone as a putative plastidic ATP-binding-cassette (ABC) protein homologue. The soybean GmNAP1 protein contains an N-terminal transit peptide which targets it into the chloroplast. The transcription level of the GmNAP1 gene was investigated under continuous red light, continuous far-red light, and complete darkness. The main function of this NAP1 protein is the transport of protoporphyrin IX which is the precursor of chlorophyll from the cytoplasm to the chloroplast. The GmNAP1 gene was transferred into the Arabidopsis under the CaMV 35S promoter. The chlorophyll level of this transgenic Arabidopsis plant was much higher than the chlorophyll level of the wild type Arabidopsis plant.
Keywords
Nonintrinsic ABC protein; chlorophyll; protoporphyrin X; soybean;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Sanchez-Fernandez, R., T. G. E. Davies, J. O. D. Coleman, and P. A. Rea. 2001. The Arabidopsis thaliana ABC protein superfamily, a complete inventory. J. Biol. Chem. 276, 30231-30244.   DOI
2 Seo, K. Y, Y. H. Son, and J. W. Koo. 2005. Extraction and determination of chlorophyll contents of Korean pine needles using acetone and DMSO (dimethylsulfoxide). J. Korean For. Soc. 4, 264-268.
3 Strand, A., T. Asami, J. Alonso, J. R. Ecker, and J. Chory. 2003. Chloroplast to nucleus communication triggered by accumulation of Mg-protoporphyrin IX. Nature 421, 79-82.   DOI
4 Takahashi, Y. and U. Tokumoto. 2002. A third bacterial system for the assembly of iron-sulfur clusters with homologs in archaea and plastids. J. Biol. Chem. 277, 28380-28383.   DOI
5 Xu, X. M., S. Adams, N. H. Chua, and S. G. Moller. 2005. AtNAP1 represents an atypical SufB protein in Arabidopsis plastids. J. Biol. Chem. 280, 6648-6654.   DOI
6 Xu, X. M. and S. G. Møller. 2004. AtNAP7 is a plastidic SufC-like ATP-binding cassette/ATPase essential for Arabidopsis embryogenesis. Proc. Natl. Acad. Sci. 101, 9143-9148.   DOI
7 Hedtke, B., I. Wagner, T. Borner, and W. R. Hess. 1999. Inter-organellar crosstalk in higher plants: impaired chloroplast development affects mitochondrial gene and transcript levels. Plant J. 19, 635-643.   DOI
8 Holland, B. I. and M. A. Blight. 1999. ABC-ATPases, adaptable energy generators fuelling transmembrane movement of a variety of molecules in organisms from bacteria to humans. J. Mol. Biol. 293, 381-399.   DOI
9 Keegstra, K. and K. Cline. 1999. Protein import and routing systems of chloroplasts. Plant Cell 11, 557-570.   DOI
10 Larkin, R. M., J. M. Alonso, J. R. Ecker, and J. Chory. 2003. GUN4, a regulator of chlorophyll synthesis and intracellular signaling. Science 299, 902-906.   DOI
11 Loiseau, L., S. Ollagnier-de-Choudens, L. Nachin, M. Fontecave, and F. Barras. 2003. Biogenesis of Fe-S cluster by the bacterial Suf system: SufS and SufE form a new type of cysteine desulfurase. J. Biol. Chem. 278, 38352-38359.   DOI
12 Moller, S. G., T. Kunkel, and N. H. Chua. 2001. A plastidic ABC protein involved in intercompartmental communication of light signaling. Genes Dev. 15, 90-103.   DOI
13 Nachin, L., L. Loiseau, D. Expert, and F. Barras. 2003. SufC: an unorthodox cytoplasmic ABC/ATPase required for [Fe-S] biogenesis under oxidative stress. EMBO J. 22, 427-437.   DOI
14 Osteryoung, K. W. and J. Nunnari. 2003. The division of endosymbiotic organelles. Science 302, 1698-1704.   DOI
15 Rangachari, K., C. T. Davis, J. F. Eccleston, E. M. A. Hirst, and J. W. Saldanha. 2002. SufC hydrolyzes ATP and interacts with SufB from Thermotoga maritima. FEBS Lett. 74, 225-228.   DOI
16 Rea, P. A. 2007. Plant ATP-Binding Cassette Transporters. Annu. Rev. Plant Biol. 58, 347-375.   DOI   ScienceOn
17 Dassa, E. and P. Bouige. 2001. The ABC of ABCs: a phylogenetic and functional classification of ABC systems in living organisms. Res. Microbiol. 152, 211-229.   DOI