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http://dx.doi.org/10.5352/JLS.2010.20.10.1511

Isolation and Identification of Inhibitory Compounds on Helicobacter pylori from Rosa multiflora Thunberg Fruit Extracts  

Park, Ki-Tae (Department of Food Engineering, Kyungpook National University)
Kim, Jin-Sung (Department of Food Engineering, Kyungpook National University)
Jo, Bun-Sung (Department of Food Engineering, Kyungpook National University)
An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Hanny University)
Chun, Sung-Sook (NIP Biotech)
Kim, Jeung-Hoan (NIP Biotech)
Cho, Young-Je (School of Applied Biosciences, Kyungpook National University)
Publication Information
Journal of Life Science / v.20, no.10, 2010 , pp. 1511-1518 More about this Journal
Abstract
The antimicrobial activity of 70% ethanol extracts from Rosa multiflora Thunberg fruit against Helicobacter pylori was examined. The inhibitory activity of Rosa multiflora Thunberg fruit extracts against H. pylori was determined to clear a zone of 14 mm with 70% ethanol extracts. Purification of inhibitory compounds was carried on Sephadex LH-20 and $C_{18}$ cartridge column chromatography using a gradient procedure, with increasing ethanol ($0{\rightarrow}100%$) in $H_2O$. The chemical structure of the purified inhibitory compounds on H. pylori was identified to be protocatechuic acid, chlorogenic acid and quercetin by FAB-MS, NMR and IR spectrum.
Keywords
Helicobacter pylori; Rosa multiflora Thunberg fruit; isolation; identification; inhibitory compounds;
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