Browse > Article
http://dx.doi.org/10.5352/JLS.2010.20.10.1498

Identification of Polymorphisms in CAST Gene Associated with Economic Traits in Hanwoo (Bos taurus coreanae)  

Oh, Jae-Don (Genomic Informatics Center, Hankyong National University)
Lee, Jin-A (Genomic Informatics Center, Hankyong National University)
Lee, Kun-Woo (Genomic Informatics Center, Hankyong National University)
Park, Kyung-Do (Genomic Informatics Center, Hankyong National University)
Cho, Byung-Wook (School of Bio-Resources, Pusan National University)
Jeon, Gwang-Joo (Genomic Informatics Center, Hankyong National University)
Lee, Hak-Kyo (Genomic Informatics Center, Hankyong National University)
Kong, Hong-Sik (Genomic Informatics Center, Hankyong National University)
Publication Information
Journal of Life Science / v.20, no.10, 2010 , pp. 1498-1504 More about this Journal
Abstract
A number of studies have shown that the calpain system is important in normal skeletal muscle growth. An increased rate of skeletal muscle growth can result from a decreased rate of muscle protein degradation, and this is associated with a decrease in activity of the calpain system, due principally to a large increase in calpastatin (CAST) activity. The CAST gene, mapped to BTA 7, is considered a candidate gene for beef tenderness and muscle growth. The present study used comparative sequencing of five novel polymorphisms located within exon 20 and 22 of the bovine CAST gene in Hanwoo: exon20- 109737G/A, 109749T/C, 109823T/C, exon22- 116151G/A, intron- 109926G/A. The association of the CAST SNPs with economic traits was studied. The 109926G/A showed a significant effect only on the longissimus muscle area (LMA, p<0.05) in Hanwoo. 109926G/A with the genotype GG had a significantly higher effect on LMA (75.35) than the genotype AA (69.6, p<0.05). Also, the 116151G/A showed a significant effect only on weight at 18 months (W18, p<0.05). 116151G/A with the genotype GG had a significantly higher effect on W18 (428.54) than the genotype AA (408.87, p<0.05).
Keywords
Calpastatin; CAST gene; SNP; Hanwoo;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Lang, C. H., R. A. Frost, and T. C. Vary. 2007. Regulation of muscle protein synthesis during sepsis and inflammation. Am. J. Physiol. Endocrinol. Metab. 293, E453-459.   DOI
2 Lee, H. J., S. H. Lee, Y. M. Cho, H. B. Yoon, B. K. Jeon, S. J. Oh, M. S. Kwon, and D. H. Yoon. 2004. Association Between the Polymorphism on Intron 5 of the Lipoprotein Lipase Gene and Carcass Traits in Hanwoo (Korean cattle). J. Anim. Sci. & Technol. (Korean) 46, 947-956.   DOI
3 Lonergan, S. M., C. W. Ernst, M. D. Bishop, C. R. Calkins, and M. Koohmaraie. 1995. Relationship of restriction fragment length polymorphisms (RFLP) at the bovine calpastatin locus to calpastatin activity and meat tenderness. J. Anim. Sci. 73, 3608-3612.
4 Mellegren, R. L. 1997. Evidence for participation of a calpain-like cysteine protease in cell progression through the late G1 phase. Biochemical and Biophysical Research Communications 236, 555-558.   DOI   ScienceOn
5 Nonneman, D., S. M. Kappes, and M. Koohmaraie. 1999. Rapid communication: a polymorphic microsatellite in the promoter region of the bovine calpastatin gene. J. Anim. Sci. 77, 3114-3115.
6 Oreopoulos, A., R. Padwal, K. Kalantar-Zadeh, G. C. Fonarow, C. M. Norris, and F. A. McAlister. 2008. Body mass index and mortality in heart failure: a meta-analysis. Am. Heart J. 156, 13-22.   DOI
7 Schenkel, F. S., S. P. Miller, Z. Jiang, I. B. Mandell, X. Ye, H. Li, and J. W. Wilton. 2006. Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle. J. Anim. Sci. 84, 291-299.
8 Ha, O. K., J. S. Choi, S. W. Kim, Y. I. Choi, S. S. Lee, J. W. Choi, S. H. Jeon, and K. S. Kim. 2009. Commercial Application of Porcine MC1R Gene Polymorphisms to Korean Pork Industry. J. Anim. Sci. & Technol. (Korean) 51, 193-200.   DOI
9 Hasselgren, P. O., M. J. Menconi, M. U. Fareed, H. Yang, W. Wei, and A. Evenson. 2005. Novel aspects on the regulation of muscle wasting in sepsis. Int J. Biochem. Cell Biol. 37, 2156-2168.   DOI
10 Hund, E. F. 1996. Neuromuscular complications in the ICU: the spectrum of critical illness-related conditions causing muscular weakness and weaning failure. J. Neurol. Sci. 136, 10-16.   DOI
11 Hund, E. 2001. Neurological complications of sepsis: critical illness polyneuropathy and myopathy. J. Neurol. 248, 929-934.   DOI
12 Jorgensen, J. O. L., S. A. Pedersen, L. Thuesen, J. Jorgensen, T. Ingemann-Hansen, and N. E. Shakkebaek. 1989. Beneficial effects of growth hormone treatment in growth hormone deficient adults. Lancet 1, 1221-1225.
13 Juszczuk-Kubiak, E., T. Sakowski, K. Flisikowski, K. Wicinska, J. Oprzadek, and S. J. Rosochacki. 2004. Bovine mu-calpain (CAPN1) gene: new SNP within intron 14. J. Appl. Genet. 45, 457-460.
14 Kong, H. S., J. D. Oh, J. H. Lee, D. H. Yoon, Y. H. Choi, B. W. Cho, H. K. Lee, and G. J. Jeon. 2007. Association of sequence variations in DGAT 1 gene with economic traits in Hanwoo (Korea cattle). Asian-Aust. J. Anim. Sci. 20, 817-820.   DOI
15 Kuryl, J., W. Kapelanki, M. Pierzchała, S. Grajewska2, and M. Bocian. 2003. Preliminary observations on the effect of calpastatin gene (CAST) polymorphism on carcass traits in pigs. Anim. Sci. Papers and Reports 2, 87-95.
16 Chung, H. Y., M. E. Davis, and H. C. Hines. 2001. Genetic variants detected by PCR-RFLP in intron 6 of the bovine calpastatin gene. Anim. Genet. 32, 53.   DOI
17 Kutner, N. G. and R. Zhang. 2001. Body mass index as a predictor of continued survival in older chronic dialysis patients. Int. Urol. Nephrol. 32, 441-448.   DOI
18 Cho, S., T. S. Park, D. Yoon, H. S. Cheong, S. Namgoong, B. L. Park, H. W. Lee, C. S. Han, E. M. Kim, I. C. Cheong, H. Kim, and H. D. Shin. 2008. Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with back fat thickness in Korean native cattle. BMB. Rep. 41, 29-34.   DOI
19 Chung, H. Y., M. E. Davis, and H. C. Hines. 1999. A DNA polymorphism of the bovine calpastatin gene detected by SSCP analysis. Anim. Genet. 30, 80.   DOI
20 Cockett, N. E., T. L. Shay, R. D. Green, and D. L. Hancock. 1995. Rapid communication: a TaqI restriction fragment length polymorphism in the bovine calpastatin gene. J. Anim. Sci. 73, 3790.
21 Cote, C. G. and B. R. Celli. 2007. Predictors of mortality in chronic obstructive pulmonary disease. Clin. Chest Med. 28, 515-524.   DOI
22 Cuneo, R. C., F. Salomon, C. M. Wiles, and P. H. Sonksen. 1990. Skeletal muscle performance in adults with growth hormone deficiency. Horm. Res. 33, 55-60.   DOI
23 Cuneo, R. C., F. Salomon, C. M. Wiles, J. M. Round, R. Hesp, and P. H. Sonksen. 1992. Histology of skeletal muscle in adults with growth hormone deficiency: Comparison with normal muscle and response to growth hormone treatment. Horm. Res. 37, 23-28.   DOI
24 Garrouste-Orgeas, M., G. Troche, E. Azoulay, A. Caubel, A. de Lassence, and C. Cheval. 2004. Body mass index. An additional prognostic factor in ICU patients. Intensive Care Med. 30, 437-443.   DOI
25 Barnoy, S., T. Glasre, and N. S. Kosower. 1997. Calpain and calpastatin in myoblast defferentiation and fusion effects of inhibitors. Biochim. Biophys. Acta 1358, 181-188.   DOI   ScienceOn
26 Geesink, G. H. and M. Koohmaraie. 1999. Effect of calpastatin on degradation of myofibrillar proteins by mu-calpain under postmortem conditions. J. Anim. Sci. 77, 2685-2692.
27 Goll, D. E., V. F. Thompson, R. G. Taylor, and A. Ouali. 1998. The calpain system and skeletal muscle growth. Canadian J. Anim. Sci. 78, 503-512.   DOI
28 Balcerzak, D., S. Poussard, J. J. Brutis, N. Flamrani, M. Soriano, P. Cottin, and A. Ducastaing. 1995. An antisense oligonucleotide to m-calpain mRNA inhibits myoblast fusion. J. Cell Sci. 108, 2077-2082.
29 Cheong, H. S., D. Yoon, L. H. Kim, B. L. Park, H. W. Lee, C. S. Han, E. M. Kim, H. Cho, E. R. Chung, I. Cheong, and H. D. Shin. 2007. Titin-cap (TCAP) polymorphisms associated with marbling score of beef. Meat Sci. 77, 257-263.   DOI
30 Bishop, M. D., J. Koohmaraie, J. Killefer, and S. Kappes. 1993. Rapid communication: restriction fragment length polymorphisms in the bovine calpastatin Gene. J. Anim. Sci. 71, 2277.