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http://dx.doi.org/10.5352/JLS.2010.20.10.1490

Effects of Cadmium Exposure on Tissues of Carassius auratus  

Shin, Myung-Ja (Department of Biological Science, Andong National University)
Kwon, O-Chang (Department of Biological Science, Andong National University)
Lee, Jong-Eun (Department of Biological Science, Andong National University)
Seo, Eul-Won (Department of Biological Science, Andong National University)
Publication Information
Journal of Life Science / v.20, no.10, 2010 , pp. 1490-1497 More about this Journal
Abstract
The present study aimed to investigate the level of accumulated heavy metal in various tissues of Carassius auratus after exposure to Cadmium (Cd), histologically and physiologically. After treating C. auratus with Cd, the accumulated Cd in gill tissues was detected to be of the highest content, and showed the lowest content in integument tissues. Also, Cd content increased in a time dependent manner and showed the highest accumulation in the tissues exposed for 20 days. Antioxidant enzyme activities showedhigher activity in the gill and integument than in the kidney and liver tissues. In the case of SOD, antioxidation activity of SOD in all Cd exposed tissues was higher than in unexposed tissues. The activities of SOD and CAT also became higher after Cd exposure. Gill tissues exposed to Cd showed an increased number of mucous cells between lamella in a time dependent manner. In addition, the gills showed morphological changes such as edema, exfoliation of epithelial cells, and fusion of the secondary lamellae. Also, exposure to Cd for 20 days had an effect on gill tissues, causing membrane damage in the mitochondria and nucleus. In kidney tissues, atrophied glomerulus was observed, and the empty space in Bowman's capsule was wider.
Keywords
Cadmium; antioxidant enzyme; heavy metal; Carassius auratus;
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1 Schmitt, C. J. and G. M. Dethloff. 2000. Biomonitoring of environmental status and trends (BEST) program, selected methods for monitoring chemical contaminants and their effects in aquatic ecosystems. U. S. Geological Survey, Biological Resources Division, Information and Technology Report, USGS/ BRD/ITR-2000-0005.
2 Sorensen, E. M. 1991. Cadmium. In: Metal Poisoning in Fish. pp. 175-234, CRC press Inc., Boston.
3 Stacey, N. H. and C, D. Klaassen. 1981. Comparison of the effects of metals on cellular injury and lipid peroxidation in isolated rat hepatocyte. J. Toxicol. Environ. Hlth. 7, 139.   DOI
4 Webb, M. and A. T. Etienne. 1977. Studies on the toxicity and metabolism of cadmium thionein. Biochem. Pharmacol. 26, 25-30.   DOI
5 Wendel, A. and S. Feuerstin. 1981. Drug-induced lipid peroxidation in mice-l. Modulation by monoxygenase activity, glutathione and selsnium status. Biochem. Pharmacol. 30, 2513-2520.   DOI
6 Faeder, E. J., S. Q. Chanet, and L. C. King. 1977. Biochemical and ultrastructural changes in livers of cadmium treated rats. Toxicol. Appl. Pharmacol. 39, 473-483.   DOI
7 Fennel, R. H. and V. M. Pardo. 1967. Experimental glomerulonephritis in rats. Lab. Invest. 17, 483-488.
8 Flohe, L., A. Wolfgang, and W. A. Gunzler. 1984. Assay of glutathione peroxidase, pp. 105-114, In Packer, L. (ed.), Methods in enzymatic analysis, Academic Press Inc., New York.
9 Friberg, L. and J. Vostal. 1972. Mercury in the environment, pp. 17-23, CRS Press Inc., Cleveland.
10 Goyer, R. A. 1986. Toxic effect of metals. Casarett and Doull’s Toxicology, pp. 623-680, In Klaassen, C. D., M. D. Amder, and J. Doull (eds.), Mecmillian Publishing Co., New York.
11 Kim, J. S., M. J. Shin, J. E. Lee, and E. W. Seo. 2009. Heavy metal contents in tissues of Carassius auratus in Andong and Imha reservoir. J. Life Sci. 19, 1562-1567.   DOI
12 Lee, J. S., J. C. Kang, and Y. K. Shin. 2001. Histological responses of the flounder, Paralichthys olivaceus exposed to copper. J. Fish Pathol. 14, 81-90.
13 McCord, J. M. and I. Fridovich. 1969. Superoxide dismutase an enzymic function ferythrocuprotein (Hemocuprotein). J. Biol. Chem. 244, 6049-6055.
14 Muller, M. E., D. A. Sanchez, H. L. Bergman, D. G. Rhem, and C. M. Wood. 1991. Nature and time course of acclimation to aluminium in juvenile brook trout (Salvelinus fontinalis). Gill histology. Can. J. Fish Sci. 48, 2016-2027.   DOI
15 Park, S. H., I. S. Song, and Y. C. Cho. 2000. Experimental study on the accumulation of cadmium and other metals in the fish bodies (Oryzias latipes). Korean J. Environ. Hlth. Soc. 26, 25-31.
16 Probes, G. S., W. F. Bousquet, and T. S. Miya. 1977. Kinetics of cadmium-induced hepatic and renal metallothionein synthesis in the mouse. Toxicol. Appl. Pharmacol. 39, 51-60.   DOI
17 Aebi, H. 1984. Catalase in vitro, pp. 121-126, In Packer, L. (ed.), Methods in Enzymology, 105, Academic Press Inc., New York.
18 APHA-AWWA-WPCF. 1985. Standard methods for the examination of water and wastewater. pp. 715-742. 16th eds., Washington, D. C.
19 Bae, H. K., E. K. Kim, S. S. Nam, C. K. Moon, S. H. Jeon, J. G. Na, and K. S. Park. 2002. A study on cadmium accumulations and histopathological changes in the gills of Crucian Carp (Carassius auratus). Korean J. Environ. Toxicol. 17, 53-61.
20 Chance, B., H. Siec, and A. Boveris. 1979. Hydroperoxide metabolism in mammalian organs. Physiol. Rev. 59, 527-605.
21 Cho, K. S., J. H. Park, and J. C. Kang. 2004. Acute toxicity of Carassius auratus and Pungtungia herzi lavra on mercury, lead and copper exposure. J. Korean Soc. on Water Quality 20, 265-268.
22 Cho, Y. G. and G. B. Kim. 2007. Bioaccumulation of Pb and Cd in Blue mussel (Mytilus edulis) and Oliver flounder (Paralichthys olivaceus) exposed to rearing media. J. Korean Soc. for Marine Eviron. Eng. 10, 21-28.
23 Dudkey, R. E., D. J. Sovovoda, and C. D. Klaassen. 1982. Acute exposure to cadmium causes servere liver injury in rats. Toxicol. Appl. Pharmacol. 65, 302-313.   DOI
24 EPA. 1978. Criteria and rationale for decision making in aquatic hazard evalution, aquatic hazard of pesticides task group, Amer. Inst. Biol. Science, pp. 46, Arlinton, Virginia.
25 EPA. 1989. Short-term method for estimating the chronic toxicity of effluent and receiving water to freshwater organism, U.S. EPA-600/4-89-001.