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http://dx.doi.org/10.5352/JLS.2009.19.9.1314

Evaluation of Cell Death and the Reduction of ERK Phosphorylation in Non-Small Cell Lung Cancer Cells after Exposure to Sodium Butyrate  

Park, Ji-Eun (Department of Anatomy and Cell Biology, College of Medicine, Dong-A University)
Lee, Seung-Gee (Department of Anatomy and Cell Biology, College of Medicine, Dong-A University)
Lim, Hyun-Ju (Department of Anatomy and Cell Biology, College of Medicine, Dong-A University)
Kim, Ji-Young (Department of Anatomy and Cell Biology, College of Medicine, Dong-A University)
Chung, Jin-Yong (Department of Anatomy and Cell Biology, College of Medicine, Dong-A University)
Kim, Yoon-Jae (Department of Anatomy and Cell Biology, College of Medicine, Dong-A University)
Lee, Chang-Hun (Department of Pathology, Pusan National University School of Medicine)
Lee, Min-Ki (Department of Internal Medicine, Pusan National University School of Medicine)
Yoo, Ki-Soo (Department of Anatomy and Cell Biology, College of Medicine, Dong-A University)
Yoo, Young-Hyun (Department of Anatomy and Cell Biology, College of Medicine, Dong-A University)
Kim, Jong-Min (Department of Anatomy and Cell Biology, College of Medicine, Dong-A University)
Publication Information
Journal of Life Science / v.19, no.9, 2009 , pp. 1314-1320 More about this Journal
Abstract
Histone deacetylase inhibitor (HDACI) is a new promising candidate as an antineoplastic agent for the treatment of solid and hematologic malignancies. In order to evaluate cell death and to elucidate the related mechanism(s) in NSCLC cells after HDACI, sodium butyrate (SB), a representative HDACI, was used to treat H460 cells for 48 hrs. SB exposure resulted in a significant reduction of cell viability at concentrations below 7.5 mM, and about 50% of cell death occurred at 20 mM. The types of cell death induced by SB were both apoptosis and necrosis, evaluated by Annexin-V staining combined with propidium iodide. SB treatment significantly evoked G2/M cell cycle arrest and subsequently induced cell death with caspase-dependent manner. While ERK protein content was not altered after SB, phosphorylated forms of ERK were markedly reduced. Taken together, SB is significantly able to induce cell death in NSCLC cell line H460, and it is suggested that the reduction of ERK phosphorylation might be closely involved in the cancer cell death mechanism initiated by HDACI.
Keywords
Sodium butyrate; apoptosis; caspase; p53; ERK; lung cancer cell;
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