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http://dx.doi.org/10.5352/JLS.2009.19.9.1294

Synergistic Cytotoxic Effects by Combination Treatment of Genistein and Daidzein in Human Colorectal Cancer Cell  

Son, Seong-Min (Dept. of Biological Sciences, Andong National University)
Lim, Seung-Hyun (Dept. of Biological Sciences, Andong National University)
Kim, Hyo-Rim (Dept. of Biological Sciences, Andong National University)
Kim, Min-Jeong (Dept. of Biological Sciences, Andong National University)
Kim, Tae-Wan (Dept. of Food Science and Biotechnology, Andong National University)
Lee, Jong-Hwa (Dept. of Food Science and Biotechnology, Andong National University)
Kim, Jong-Sik (Dept. of Biological Sciences, Andong National University)
Publication Information
Journal of Life Science / v.19, no.9, 2009 , pp. 1294-1298 More about this Journal
Abstract
To investigate whether isoflavone genistein and daidzein could affect cancer cell viability, human colorectal HCT116 cells were incubated with genistein or daidzein in a dose-dependent manner. Genistein decreased cancer cell viability in a dose-dependent manner, whereas daidzein did not show dramatic cytotoxic effects. We also found that 71 genes were up-regulated more than 2-fold, whereas 64 genes were down-regulated more than 2-fold with 24 hr of $50{\mu}M$ genistein treatment by our previous microarray data. Among the up-regulated genes, we selected 3 genes (DKK1, ATF3 and NAG-1) and performed RT-PCR to confirm microarray data. The results of RT-PCR were highly correlated with those of the microarray experiment. In addition, we investigated whether a combination treatment of genistein and daidzein could affect cancer cell viability. Surprisingly, the combination treatment did show synergistic cytotoxic effects detected by MTS assay. The results of RT-PCR and real-time PCR indicate that a combination of genistein and daidzein can synergistically induce NAG-1 expression in HCT116 cells. This result implies that NAG-1 induction is highly associated with synergistic cytotoxic effects induced by a combination treatment of genistein and daidzein. Overall, these results may provide a clue in explaining the anti-cancer activity of soy bean in human colorectal cancer.
Keywords
Cancer chemoprevention; genistein; daidzein; combination treatment; NAG-1;
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