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http://dx.doi.org/10.5352/JLS.2009.19.8.1009

In vitro Anti-proliferative Characteristics of Flavonoids and Diazepam on MDA-MB-231 Breast Cancer Cells  

Kim, Ji-Kwan (Department of Pharmacology, School of Medicine, Kyungpook National University)
Lee, Maan-Gee (Department of Pharmacology and 1Nuclear Medicine, School of Medicine, Kyungpook National University)
Lee, Jae-Tae (Department of Nuclear Medicine, School of Medicine, Kyungpook National University)
Ha, Jeoung-Hee (Department of Pharmacology, School of Medicine, Kyungpook National University)
Publication Information
Journal of Life Science / v.19, no.8, 2009 , pp. 1009-1015 More about this Journal
Abstract
The beneficial use of sedatives is often required for medically ill patients. This study examined the effect of plant flavonoids and diazepam peripheral-type benzodiazepine receptor (PBR) activation and glucose utilization in breast cancer cells, along with their interactions. In estrogen receptor negative MDA-MB-231 cells, the anti-proliferative activity of fisetin (3,7,3',4'-tetrahydroxyflavone) and diazepam was more prominent than in estrogen receptor positive MCF-7 cells. Unlike PBR ligands, treatment with $10^{-6}$ M concentration of diazepam for 3 days exhibited anti-proliferative effects, while similar to apigenin (4',5,7-Trihydroxyflavone) and fisetin, diazepam hardly affected the PBR mRNA expression by MDA-MB-231 cells. Treatment with $10^{-6}$ M concentration of flavonoids and diazepam for 3 days inhibited the glucose utilization of MDA-MB-231 cells. Treatment with $10^{-6}$ M concentration of flavonoids and diazepam for 6 days showed increased cytotoxicity and reduced the PBR mRNA expression of the MDA-MB-231 cells. Apigenin enhanced diazepam-induced anti-proliferative effects on the MDA-MB-231 cells as well. All together, this study showed the in vitro anti-proliferative activity of flavonoids and diazepam on MDA-MB-231 breast cancer cells, plus additive enhancements. In conclusion, this study provides experimental basis for advanced trials in the future.
Keywords
Flavonoids; diazepam; anti-proliferation; glucose utilization; breast cancer cells;
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