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http://dx.doi.org/10.5352/JLS.2009.19.7.963

Neuroprotective Effect of Extracts from Root Bark of Morus alba on Glutamate-induced Cytotoxicity in Neuronal Cells.  

Kim, Hyun-Jung (Department of Food Science and Biotechnology, Kyungnam University)
Kim, Ji-Hyun (Department of Food Science and Biotechnology, Kyungnam University)
Son, Eun-Soon (Department of Food Science and Biotechnology, Kyungnam University)
Lee, Jeung-Min (Department of Food Science and Biotechnology, Kyungnam University)
Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
Publication Information
Journal of Life Science / v.19, no.7, 2009 , pp. 963-967 More about this Journal
Abstract
This study evaluated the neuroprotective effect of extracts from the root bark of Morus alba (MA) against glutamate-induced cytotoxicity in neuronal cells. Glutamate-induced cytotoxicity was shown by MTT reduction assay. The neuroprotective effects of methanol, ethanol, and acetone extracts from MA against glutamate-induced cytotoxicity were measured. Among the three extracts, the methanolic extracts showed the highest protective effect, as determined by the results of an morphological assay, a lactate dehydrogenase release assay. Furthermore, the methanol extracts were fractionated sequentially with hexane, diethyl ether, ethyl acetate, and water layer according to degree of polarity. The hexane fractions exhibited a neuroprotective effect against glutamate-stressed N18-RE-105 cells. Therefore, these results suggest that extracts of MA could be a new potential candidate as a protective substance against glutamate-induced cytotoxicity.
Keywords
Neuroprotective effect; Morus alba; glutamate; cytotoxicity; N18-RE-105 cell;
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