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http://dx.doi.org/10.5352/JLS.2009.19.7.905

Microarray Analysis of Gene Expression Affected by Water-extracts of Pinelliae rhizoma in a Hypoxic Model of Cultured Rat Cortical Cells  

Kwon, Gun-Rok (Dept. of Oriental Medicine, Dongguk University)
Jung, Hyun-Jung (Dept. of Oriental Medicine, Dongguk University)
Shin, Gil-Jo (Dept. of Oriental Medicine, Dongguk University)
Moon, Il-Soo (Department of Anatomy, Dongguk University)
Lee, Won-Chul (Pusan National University School of Oriental Medicine)
Jung, Seung-Hyun (Dept. of Oriental Medicine, Dongguk University)
Publication Information
Journal of Life Science / v.19, no.7, 2009 , pp. 905-916 More about this Journal
Abstract
Pinelliae rhizoma (Pr, 半夏) is a traditional medicine used in the treatment of incipient stroke. We investigated the effects of Pr on gene expression in a hypoxic model using cultured rat cortical cells. Pr (2.5 $\mu$g/ml) was added to the culture medium on DIV 12. A hypoxic shock (2% 0$_2$/5% CO$_2$, 37$^{\circ}$C, 3 hr) was given two days later (on DIV 14), and total mRNAs were isolated at 24 hr post-shock from both Pr-treated samples and untreated control cultures. Microarray using TwinChip $^{TM}$ Rat-5K (Digital Genomics, Seoul) indicated that Pr upregulated genes for cell growth and differentiation (tubb5, tgfa, ptpn11, n-ras, pdgfa) and antiapoptosis (mcl-1), while downregulating the apoptosis-induced gene (tieg). Therefore, it is interpreted that Pr protects neurons from hypxoic shock by maintaining cell growth and differentiation and by preventing apoptosis.
Keywords
Cortical cell culture; hypoxia; microarray; Pinelliae rhizoma;
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