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http://dx.doi.org/10.5352/JLS.2009.19.6.787

Identification of Microorganisms Isolated from Jeju Coastal Sedimentary Layer and Characterization of Their Proteases.  

Kim, Man-Chul (Department of Aquatic Life Medicine, Jeju National University)
Jang, Tae-Won (Department of Aquatic Life Medicine, Jeju National University)
Kim, Ju-Sang (Department of Aquatic Life Medicine, Jeju National University)
Han, Yong-Jae (Department of Aquatic Life Medicine, Jeju National University)
Harikrishnan, Ramasamy (Department of Aquatic Life Medicine, Jeju National University)
Han, Song-Hun (Department of Aquatic Life Medicine, Jeju National University)
Oh, Duck-Chul (Department of Life Science, Jeju National University)
Heo, Moon-Soo (Department of Aquatic Life Medicine, Jeju National University)
Publication Information
Journal of Life Science / v.19, no.6, 2009 , pp. 787-792 More about this Journal
Abstract
In this study, protease-producing bacteria were isolated from the marine sedimentary layer in coastal Jeju. We isolated 2 protease producing strains (SK-2 and SK-125) and tested their protesase producing activities. Gram staining and BIOLOG of isolated strains revealed that strains SK-2 and SK-125 belong to Bacillus and Pseudoalteromonas families, respectively. The 16S rDNA nucleotide sequences analyses of the isolated strains showed 99% sequence homology with those of Bacillus sp. and Pseudoalteromonas sp.; therefore, the isolated strains SK-2 and SK-125 were named Bacillus sp. SK-2 and Pseudoalteromonas sp. SK-125, respectively. The optimum conditions for the cell growth of protease activities were obtained when the both isolates were cultured at $30^{\circ}C$, 96 hrs and pH $7{\sim}8$.
Keywords
Protease; marine sedimentary layer; Bacillus sp.; Pseudoalteromonas sp.;
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