Browse > Article
http://dx.doi.org/10.5352/JLS.2009.19.5.652

Antioxidative Activity of Aralia elata Shoot and Leaf Extracts  

Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co., Ltd.)
Ahn, Hee-Young (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Eom, Kyung-Eun (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Park, Bo-Kyung (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Jun, Bang-Sil (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Cho, Young-Su (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
Publication Information
Journal of Life Science / v.19, no.5, 2009 , pp. 652-658 More about this Journal
Abstract
The comparative activities of aqueous, ethanol, and methanol extracts from Aralia elata shoot (AES) and leaf (AEL) were tested by in vitro experimental models of linoleic acid peroxidation by thiocyanate and thiobarbituric acid (TBA) methods and scavenging activities of free radicals by DPPH (${\alpha}$,${\alpha}$'-diphenyl-${\beta}$-picrylhydrazyl). In addition, bio-active materials (phenolic compounds and minerals) were also measured. The extract yield of each solvent extracted from AES and AEL was 3.08% and 3.13% in aqueous, 0.58% and 0.66% in ethanol, and 0.81% and 1.73% in methanol, respectively. The highest extract yield was found in the aqueous extract from AEL. Major mineral contents (mg%) of AES and AEL were 575.7 and 759.3 in Ca, 353.5 and 330.0 in K, and 31.3 and 31.0 in Mg, respectively. The highest free radical scavenging activity was found in the aqueous extract by 28.69% at 0.1% additional level from AES and in the methanol extract by 92.36% at 0.1% additional level from AEL. Free radical scavenging activity was stronger in AEL than in AES. In antioxidative activities determined by thiocyanate and TBA methods against lipid peroxidation using linoleic acid, ethanol extracts from AEL showed the highest antioxidative activity at all treatment concentrations. These results may provide the basic data to understand the biological activities of bio-active materials derived from AES and AEL.
Keywords
Aralia elata; antioxidative activity; lipid peroxidation; DPPH(${\alpha}$,${\alpha}$'-diphenyl-${\beta}$-picrylhydrazyl); phenolic compounds;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Yoshikawa, M., H. Matsuda, Harada, N. Murakami, J. Wamahara, and N. Murakami. 1994. Elatoside E, a new hypoglycemic principle from the root cortex of Aralia elata Seem.: structure-related hypoglycemic activity of oleanolic acid glycosides. Chem. Pharm. Bull (Tokyo) 42, 1354-1356   DOI   ScienceOn
2 Yoo, J. H., J. Y. Cha, Y. K. Jeong, K. T. Chung, and Y. S. Cho. 2004. Antioxidative effects of pine (Pinus denstifora) needle extracts. J. Life Sci. 14, 863-867   DOI
3 Song, S. J., N. Nakamura, C. M. Ma, M. Hattori, and S. X. Xu. 2001. Five saponins from the root bark of Aralica elata. Phytochemistry 56, 491-497   DOI   ScienceOn
4 Vinson, J. A. and B. A. Hontz. 1984. Phenol antioxidative index: Comparative antioxidant effectiveness of red and white wines. J. Agric. Food Chem. 43, 401-403
5 Suh, S. J., U. H. Jin, K. W. Kim, J. K. Son, S. H. Lee, K. H. Son, H. W. Chang, Y. C. 00. Lee, and C. H. Kim. 2007. Triterpenoid saponin, oleanolic acid 3-O-$\beta$-d-glucopyranosyl(1→3)-$\alpha$-l-rhamnopyranosyl(1→2)-$\alpha$-l-arabinopyranoside (OA) from Aralia elata inhibits LPS-induced nitric oxide production by down-regulated NF-κB in raw 264.7 cells. Arch. Biochem. Biophys. 497, 227-233
6 Swain, T., W. E. Hillis, and M. Oritega. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10, 83-88
7 Terao, J. 1989. Autoxidation activity of $\beta$-carotene-related carotenoids in solution. Lipids 24, 657-661
8 Wong, S. F., B. Holliwell, R. Richimond, and W. R. Skowroneck. 1981. The role of superoxide and hydroxyl radical in the degradation of hyaluronic acid induced by metal ions and by ascorbic acid. J. Inorganic Biochem. 14, 127-134   DOI   ScienceOn
9 Yang, H. K., J. Y. Jin, J. M. Kim, M. S. Ko, H. J. Hong, S. C. Kim, and Y. J. Lee. 2006. Single oral dose toxicity study of the extract of Aralia elata in mice. J. Toxical. Pub. Health 22, 439-443
10 Yoshikawa, M., T. Murakawa, E. Harada, N. Murakami, J. Yamahara, and H. Matsuda. 1996. Bioactive saponins and glycosides. VII. On the hypoglycemic principles from the root cortex of Aralia elata Seem.: structure related hypoglycemic activity of oleanolic acid oligoglycoside. Chem. Pharm. Bull (Tokyo) 44, 1923-1927   DOI   ScienceOn
11 Kuhnau, J. 1976. The flavonoids; a class of semiessential food components; their role in human nutrition. World Rev. Nutr. Diet 24, 117-120
12 Kim, Y. H. and J. G. Im. 1999. Effect of sapanin from the shoot of Aralia elata in normal rats and streptozotocin induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 28, 912-916
13 Kim, Y. S., M. R. Huh, and J. C. Park. 2001. Effects of culture media and seawater on growth and mineral concentrations in glasswort (Salicornia herbacea). Korean J. Hort. Sci. Technol. 19, 342-347
14 Koskas, J. P., J. Cillard, and P. Cillard. 1984. Autoxidation of linoleic acid and behavior of its hydroperoxides with and without tocopherols. JAOCS 61, 1467-1472
15 Lee, J. H. and S. R. Lee. 1994. Analysis of phenolic substances content on Korea plant foods. Korean J. Food Sci. Technol. 26, 310-316
16 Lee, S. O., H. J. Lee, M. H. Yu, H. G. Im, and I. S. Lee. 2005. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung Island. Korean J. Food Sci. Technol. 37, 233-243
17 Ma, S. J., B. S. Ko, and K. H. Park. 1995. Isolation of 3,4-dihydroxybenzoic acid with antimicrobial activity from bark of Aralia elata. Korean J. Food Sci. Thechnol. 27, 807-812
18 Ohkawa, H., N. Ohishi, and K. Yagi. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358   DOI   ScienceOn
19 Shin, K. H. 2006. Effects of Araliaceae on lipid levels of plasma and liver in streptozotocin-induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 35, 1172-1177   DOI   ScienceOn
20 Helmersson, J., J. Arnlov, A. Larsson, and S. Basu. 2008. Low dietary intake of beta-carotene, alpha-tocopherol and ascorbic acid is associated with increased inflammatory and oxidative stress status in a Swedish cohort. Br. J. Nutr. 15, 1-8   DOI   ScienceOn
21 Hwang, Y. P., J. H. Choi, and H. G. Jeong. 2009. Protective effect of the Aralia continentalis root extract against carbon tetrachloride-induced hepatotoxicity in mice. Food Chem. Toxicol. 47, 75-81   DOI   ScienceOn
22 Jialal, I. and S. Grundyl. 1992. Effects of dietary supplementation with alpha-tocopherol on the oxidative modification of low density lipoprotein. J. Lipid Res. 33, 899-906   DOI   ScienceOn
23 Johnson, J. E., R. Walford, D. Harma, and J. Miquel. 1986. In `Free radicals, aging and degenerative disease', Alen R. Liss, N.Y.
24 Jung, W. S., C. Y. Yu, J. G. Park, M. J. Kim, J. H. Kim, and J. K. Kim. 2006. Comparison of biological activities in extracts from Oplopanax elatus. Korean J. Medicinal Crop Sci. 14, 630-631
25 Kim, E. Y., I. H. Baik, J. H. Kim, S. R. Kim, and M. R. Rhyu. 2004. Screening of the antioxidant activity od some medicinal plants. Korean J. Food Sci. Technol. 36, 333-338
26 Kim, H. J., B. S. Jun, S. K. Kim, J. Y. Cha, and Y. S. Cho. 1998. Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctorius L.). J. Korean Soc. Food Sci. Nutr. 27, 1217-1222   과학기술학회마을
27 Kim, J. Y., Y. S. Maeng, and K. Y. Lee. 1995. Antioxidative effects of soybean extracts by using various solvents. Korean J. Food Sci. Technol. 27, 635-637
28 Kim, N. H., D. C. Yang, and A. H. Eom. 2004. A phylogenetic relationships of Araliaceae based on PCR-RAPD and ITS sequences. Korean J. Plant Res. 17, 82-93
29 Choi, M. S., D. H. Do, and D. J. Choi. 2002. The effect of mixing beverage with Aralica continentalis Kitagawa root on blood pressure and blood constituents of the diabetic and hypertensive elderly. Korean J. Food Nutr. 15, 165-172
30 Choi, H. J., W. S. Lee, S. J. Hwang, I. J. Lee, D. H. Shin, H. Y. Kim, and K. U. Kim. 2000. Changes in chemical compositions of green tea under the different extraction conditions. J. Life Sci. 10, 202-209
31 Chung, C. K. and M. E. Jung. 2003. Ethanol fraction of Aralia elata Seemann enhances antioxidant activity and lowers serum lipids in rats when administered with benzo(a)pyrene. Biol. Pharm. Bull 26, 1502-1504   DOI   ScienceOn
32 Chung, S. H., K. D. Moon, J. K. Kim, J. H. Seong, and T. H. Sohon. 1994. Changes of chemical components in persimmon leaves during growth for processing persimmon leaves tea. Korean J. Food Sci. Technol. 26, 141-146
33 Creager, M. A., T. F. Luscher, F. Cosentino, and J. A. Beckman. 2003. Diabetes and vascular disease: pathophysiology, clinical consequences, and medical therapy: Part I. Circulation 108, 1527-1532   DOI   ScienceOn
34 Han, G. J., D. S. Shin, and M. S. Jang. 2008. A study of the nutritional composition of Aralica continentalis Kitagawa and Aralica continentalis Kitagawa leaf. Korean J. Food Sci. Technol. 40, 680-685
35 Han, G. J., M. S. Jang, and D. S. Shin. 2007. Changes in the quality characteristics of Aralica continentalis Kitagawa pickle during storage. Korean J. Food Cookery Sci. 23, 294-301
36 Han, G. J. and M. S. Jang. 2008. Quality characteristics of Aralica continentalis Kitagawa leaf-kimchi as affected by storage time. J. Korean Soc. Food Sci. Nutr. 37, 1202-1207   DOI   ScienceOn
37 Cha, J. Y., J. S. Heo, B. K. Park, H. Y. Ahn, B. S. Jun, and Y. S. Cho. 2008. Antioxidative activity of zinc-enriched Saccharomyces cerevisiae FF-10 in in vitro model system. J. Life Sci. 19, 179-184
38 Cha, J. Y., H. J. Kim, C. H. Chung, and Y. S. Cho. 1999. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J. Korean Soc. Food Sci. Nutr. 28, 1310-1315
39 Cha, J. Y., J. J. Jeong, Y. T. Kim, W. S. Seo, H. J. Yang, J. S. Kim, and Y. S. Lee. 2006. Detection of chemical characteristics in Hamcho (Salicornia herbacea) according to harvest periods. J. Life Sci. 16, 683-690   DOI
40 Cha, J. Y. and Y. S. Cho. 1999. Effect of potato polyphenolics on lipid peroxidation in rats. J. Korean Soc. Food Sci. Nutr. 28, 1131-1136
41 Cha, J. Y., S. H. Park, J. S. Heo, and Y. S. Cho. 2008. Suppressive effect of administrated glutathione-enriched Saccharomyces cerevisiae FF-8 on the oxidative stress in alcoholic fatty liver. J. Life Sci. 18, 1053-1058
42 Cha, J. Y. and Y. S. Cho. 1999. Effect of potato polyphenolics on lipid peroxidation in rats. J. Korean Soc. Food Sci. Nutr. 28, 1131-1136
43 Chang, Y. S., U. Choi, D. H. Shin, and J. I. Shin. 1992. Synergistic of Rhus javanica L. ethanol extract containing several synergist. Korean J. Food Sci. Technol. 24, 149-153
44 Chen, Y. T., R. L. Zheng, Z. J. Jia, and J. Ju. 1990. Flavonoids as superoxide scavengers and antioxidants. Free Radical Biol. Med. 9, 19-21
45 Choi, U. D., H. Shin, Y. S. Chang, and J. I. Shin. 1992. Screening of natural antioxidant from plant and antioxidant effect. Korean J. Food Sci. Technol. 24, 142-148
46 Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1204   DOI   ScienceOn
47 Ahmed, K. A., M. Sekaran Muniandy, and I. S. Ismail. 2009. $N^\varepsilon$-(Carboxymethyl)lysine and Coronary Atherosclerosis-Associated Low Density Lipoprotein Abnormalities in Type 2 Diabetes: Current Status. J. Clin. Biochem. Nutr. 44, 14-27   DOI   ScienceOn
48 AOAC. 1975. official methods of analysis. 12th ed., Association of official analytical chemists. Washington, D.C., U.S.A