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http://dx.doi.org/10.5352/JLS.2009.19.1.009

Screening and Purification of an Anti-Prostate Cancer Compound, Deoxypodophyllotoxin, from Anthriscus sylvestris Hoffm  

Cho, Hyo-Jin (Department of Microbiology and Immunology, Pusan National University School of Medicine)
Yu, Sun-Nyoung (Department of Microbiology and Immunology, Pusan National University School of Medicine)
Kim, Kwang-Youn (Department of Microbiology and Immunology, Pusan National University School of Medicine)
Sohn, Jae-Hak (College of Medical and Life Sciences, Silla University)
Oh, Hyun-Cheol (College of Medical and Life Sciences, Silla University)
Ahn, Soon-Cheol (Department of Microbiology and Immunology, Pusan National University School of Medicine)
Publication Information
Journal of Life Science / v.19, no.1, 2009 , pp. 9-14 More about this Journal
Abstract
The prostate cancer is the critical health problem, increasing of its related death in worldwide. Unfortunately present surgery and chemotherapeutic choices seem to be impossible in curing or controlling prostate cancer, because metastasis occasionally advances even after these potentially curative therapies. Therefore, there is immediate need to alternative chemoprevention and chemotherapeutic agents. Over one hundred species of dried medicinal herbs were tested for proliferation inhibitory effects on prostate cancer cell line, PC-3. One of them, Anthriscus sylvestris was selected because of potent anti-proliferation effect. The dried root of A. sylvestris was extracted with 100% methanol for 2-3 days and its extract was fractionated by using ethyl acetate. And ethyl acetate layer was subjected to column chromatographies on silica gel, reverse phase-18 (RP-18) and Sephadex LH-20, in turn. Finally, the pure compound was obtained by crystallization in methanol at $4^{\circ}C$ for overnight and identified as deoxypodophyllotoxin by NMR spedorscopic and physico-chemical analyses. In addition, it was confirmed that deoxypodophyllotoxin clearly inhibits the proliferation of PC-3 cells in a dose and time-dependent manner.
Keywords
Chromatography; deoxyphyllotoxin; prostate cancer; purification; screening;
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1 Fleischer, A., A. Ghadiri, F. Dessauge, M. Duhamel, M. P. Rebollo, F. Alvarez-Franco and A. Rebollo. 2006. Modulating apoptosis as a target for effective therapy. Mol. Immunol. 43, 1065-1079   DOI   ScienceOn
2 Lowe, S. W. and A. W. Lin. 2000. Apoptosis in cancer. Carcinogenesis 21, 485-495   DOI   ScienceOn
3 Mann, J. 2002. Natural products in cancer chemotherapy: past, present and future. Nat. Rev. Cancer 2, 143-148   DOI   ScienceOn
4 Mantena, S. K., S. D. Sharma and S. K. Katiyar. 2006. Berberine, a natural product, induces G1-phase cell cycle arrest and caspase-3-dependent apoptosis in human prostate carcinoma cells. Mol. Cancer Ther. 5, 296-308   DOI   ScienceOn
5 Reddy, L., B. Odhav and K. D. Bhoola. 2003. Natural products for cancer prevention: a global perspective. Pharmacol. Ther. 99, 1-13   DOI   ScienceOn
6 Swanson, G. P., M. Riggs and J. Earle. 2004. Failure after primary radiation or surgery for prostate cancer; differences in response to androgen ablation. J. Urol. 172, 525-528   DOI   ScienceOn
7 Swanson, G. P. 2006. Management of locally advanced prostate cancer; past, present, future. J. Urol. 176, S34-S41   DOI   ScienceOn
8 Thurman, S. A., N. R. Ramakrishna and T. L. DeWeese. 2001. Radiation therapy for the treatment of locally advanced and metastatic prostate cancer. Hematol. Oncol. Clin. North Am. 15, 423-443   DOI   ScienceOn
9 Wang, G., H. Chen, M. Huang, N. Wang, J. Zhang, Y. Zhang, G. Bai, W. F. Fong, M. Yang and X. Yao. 2006. Methyl protodioscin induces G2/M cell cycle arrest and apoptosis in HepG2 liver cancer cells. Cancer Lett. 241, 102-109   DOI   ScienceOn
10 Furre, I. E., M. T. Moller, S. Shahzidi, J. M. Nesland and Q. Peng. 2006. Involvement of both caspase-dependent and -independent pathways in apoptotic induction by hexaminolevulinate-mediated photodynamic therapy in human lymphoma cells. Apoptosis 11, 2031-2042   DOI   ScienceOn
11 Gilligan, T. and P. W. Kantoff. 2002. Chemotherapy for prostate cancer. Urology 60, S94-S100   DOI   ScienceOn
12 Gordaliza, M., J. M. Miguel del Corral, M. Angeles Castro, P. A. García-García and A. San Feliciano. 2001. Cytotoxic cyclolignans related to podophyllotoxin. Farmaco. 56, 297-304   DOI   ScienceOn
13 Jemal, A., T. Murray, E. Ward, A. Samuels, R. C. Tiwari, A. Ghafoor, E. J. Feuer and M. J. Thun. 2005. Cancer statistics. 2005. CA Cancer J. Clin. 55, 10-30   DOI   ScienceOn
14 Jeong, G. S., O. K. Kwon, B. Y. Park, S. R. Oh, K. S. Ahn, M. J. Chang, W. K Oh, J. C. Kim, B. S. Min, Y. C. Kim and H. K. Lee. 2007. Lignans and coumarins from the roots of Anthriscus sylvestris and their increase of caspase-3 activity in HL-60 cells. Biol. Pharm. Bull. 30, 1340-1343   DOI   ScienceOn
15 Ko, S. H., S. H. Do, Y. S. Kwon and C. M. Kim. 1992. A Study on the chemical components from the roots of Anthriscus sylvestris. Kor. J. Pharmacogn. 23, 225-228
16 Krishnan, K., S. Campbell, F. Abdel-Rahman, S. Whaley and W. T. Stone. 2003. Cancer chemoprevention drug targets. Curr. Drug Targets 4, 45-54   DOI   ScienceOn
17 Kutuk, O. and H. Basaga. 2006. Bcl-2 protein family: implications in vascular apoptosis and atherosclerosis. Apoptosis 11, 1661-1675   DOI   ScienceOn
18 Arunkumar, A., M. R. Vijayababu, N. Srinivasan, M. M. Aruldhas and J. Arunakaran, 2006. Garlic compound, diallyl disulfide induces cell cycle arrest in prostate cancer cell line PC-3. Mol. Cell Biochem. 288, 107-113
19 Laufer, M., S. R. Denmeade, V. J. Sinibaldi, M. A. Carducci and M. A. Eisenberger. 2000. Complete androgen blockade for prostate cancer：what went wrong? J. Urol. 164, 3-9   DOI   ScienceOn
20 Abdulla, M. and P. Gruber. 2000. Role of diet modification in cancer prevention. Biofactors 12, 45-5   DOI   ScienceOn
21 Denmeade, S. R., X. S. Lin and J. T. Isaacs. 1996. Role of programmed (apoptotic) cell death during the progression and therapy for prostate cancer. Prostate 28, 251-265   DOI   ScienceOn