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http://dx.doi.org/10.5352/JLS.2009.19.1.034

A Simple Method for Isolation of Polymannuronate and Polyguluronate from Alginate Hydrolyzed by Organic Acids  

Lee, Dong-Soo (Research Center of Biotechnology & Bioengineering, KBP Co., Ltd.)
Shin, Myung-Kyo (Division of Environment and Energy, Korean Institute of Industrial Technology)
Pyeun, Jae-Hyeung (Faculty of Food Science Gild Biotechnology, Pukyong National University)
Lee, Jin-Woo (Department of Biotechnology, Dong-A University)
Publication Information
Journal of Life Science / v.19, no.1, 2009 , pp. 34-39 More about this Journal
Abstract
Alginate with a MW of 1,283 kDa was hydrolyzed with 0.4 M organic acids at $100^{\circ}C$ for 3 hr. Molecular weights of alginates hydrolyzed with organic acids ranged from 7.5 to 53.2 kDa. There was no significant difference in the molar ratio of mannuronate to guluronate in alginates hydrolyzed with organic acids. Acetic add was found to be the most effective organic acid for hydrolysis of alginate. The MW of alginate decreased with increasing concentration and reaction time with acetic acid as a hydrolyzing agent. The correlations between the MW of hydrolyzed alginate and concentration of acetic acid as well as reaction time with 0.4 M acetic acid were plotted and the relevant equations obtained in this study. Polymannuronate and polyguluronate were isolated by pH adjustment of alginate hydrolyzed with 0.4 M acetic add. The molar percentages of mannuronate in polymannuronates isolated from alginate hydrolyzed with 0.4 M acetic acid at $100^{\circ}C$ were increasing in proportional to the reaction time such as 75% for 1 hr, 90% for 3 hr, and 98% for 5 hr of reaction time.
Keywords
Alginate; hydrolysis; polymannuronate; polyguluronate; organic adds;
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Times Cited By KSCI : 1  (Citation Analysis)
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