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http://dx.doi.org/10.5352/JLS.2009.19.12.1802

Effects of Platycodin D on Gene Expressions of Pro-adipogenic and Anti-adipogenic Regulators in 3T3-L1 Cells  

Lee, Hae-Yong (Department of Microbiology, Chung-Ang University College of Medicine)
Kang, Ryun-Hwa (Department of Microbiology, Chung-Ang University College of Medicine)
Cho, Soo-Hyun (Department of Family Medicine, Yongsan Hospital)
Kim, Sung-Su (Department of Anatomy, Chung-Ang University College of Medicine)
Kim, Yeong-Shik (College of Pharmacy, Seoul National University)
Yoon, Yoo-Sik (Department of Microbiology, Chung-Ang University College of Medicine)
Publication Information
Journal of Life Science / v.19, no.12, 2009 , pp. 1802-1807 More about this Journal
Abstract
Platycodin D, a major component of Platycodi radix, is known to have various activities including anti-inflammatory, anti-hyperlipidemic, anti-tumor activities and others. Recently, it was reported that platycodin D inhibits fat accumulation and adipogenesis. The aim of this study was to investigate whether various adipogenic regulators are modulated by platycodin D treatment during the adipogenesis of 3T3-L1 cells. mRNA levels of terminal markers of adipogenesis such as ADIPOQ (adiponectin) and GLUT (glucose transporter) 4, which were quantified by real time PCR, were decreased by platycodin D treatment. mRNA expression of PPAR (peroxisome proliferator-activated receptor) $\gamma$ and C/EBP (CCAAT/enhaner binding protein) $\alpha$, which are central transcription factors of adipogenesis, were also decreased by platycodin D treatment. To elucidate the detailed molecular mechanism of platycodin D-induced inhibition of adipogenesis, we analyzed mRNA expression of upstream regulators of PPAR$\gamma$ and C/EPB$\alpha$. mRNA levels of the pro-adipogenic regulators, KROX20 and KLF (Kruppel-like factor) 15 were markedly down-regulated by platycodin D treatment. On the other hand, mRNA expression of CHOP (C/EBP homologous protein), an anti-adipogenic regulator, was significantly up-regulated by platycodin D treatment. mRNA levels of other pro-adipogenic regulators, C/EBP$\beta$ and C/EPB$\delta$, were not affected by platycodin D treatment, and another anti-adipogenic regulator, C/EBP$\gamma$ was also not affected by platycodin D treatment. Taken together, these results suggest that platycodin D-induced inhibition of adipogenesis is mediated by gene interactions including the down-regulation of pro-adipogenic regulators, KROX20 and KLF15, and the up-regulation of an anti-adipogenic regulator, CHOP.
Keywords
Adipogenesis; platycodin D; 3T3-L1; KROX20; KLF15; CHOP;
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