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http://dx.doi.org/10.5352/JLS.2009.19.10.1346

The Effects of Wnt Signaling on Neural Crest Lineage Segregation and Specification  

Song, Jin-Su (Department of Biological Sciences, College of Natural Sciences and Institute of Biotechnology, Wonkwang University)
Jin, Eun-Jung (Department of Biological Sciences, College of Natural Sciences and Institute of Biotechnology, Wonkwang University)
Publication Information
Journal of Life Science / v.19, no.10, 2009 , pp. 1346-1351 More about this Journal
Abstract
Recent evidence has shown that many pluripotetic neural crest cells are fate-restricted and that different fate-restricted crest cells emigrate from the neural tube at different times. Jin et al. (2001) identified the expression patterns of Wnts and its antagonists at the time that neural crest cells were being specified and suggested that Wnt signaling was involved in the segregation/differentiation of neural crest cells in the trunk in vitro. In this study, we evaluated the effects of Wnt signaling in avian neural crest lineage segregation. To accomplish this, Wnt signaling was disturbed at the time of neural crest segregation and differentiation by grafting Wnt-3a expressing cells and conducting dominant negative glycogen synthase kinase (dnGSK) electroporation. Stimulation of Wnt signaling induced neural crest lineage segregation and melanoblast specification, and increased the expression levels of genes known to be involved in neural crest development such as cadherin 7 and Slug, which suggests that they are involved in Wnt-induced neural crest lineage differentiation into melanoblasts.
Keywords
Cadherin; Wnt; electroporation; neural crest;
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1 Nieto, M. A, M. G. Sargent, D. G. Wilkinson, and J. Cooke. 1994. Control of cell behaviour during vertebrate development by Slug, a zinc finger gene. Science 264, 835-839   DOI
2 Nusse, R 1997. A versatile transcriptional effector of Wingless signaling. Cell 6, 1302-1306
3 Papkoff, J., B. Rubinfeld, B. Schryver, and P. Polakis. 1996. Wnt-1 regulates free pools of catenins and stabilizers APC-catenin complexes. Mol. Cell BioI. 16, 2128-2134
4 Reedy, M. V., C. D. Faraco, and C. A Erickson. 1998. The delayed entry of thoracic neural crest cells into the dorsolateral path is a consequence of the late emigration of melanogenic neural crest cells from the neural tube. Dev. BioI. 200, 234-246   DOI   ScienceOn
5 Roelink, H. and R Nusse. 1991. Expression of two members of the Wnt family during mouse development-restricted temporal and spatial patterns in the developing neural tube. Genes Dev. 5, 381-388   DOI   ScienceOn
6 Takada, K, K Yaumoto, R Takada, S. Takada, K Watanabe, T. Udono, H. Saito, K Takahashi, and S. Shibahara. 2000. Induction of melanocyte-specific microphthalmia-associated transcription factor by Wnt-3a. J. BioI. Chem. 275, 14012-14016
7 Trainor, P. and R Krumlauf. 2002. Develpoment. Riding the crest of the Wnt signaling wave. Science 297, 781-783   DOI   ScienceOn
8 Wilkinson, D. G., J. A. Bailes, and A. P. McMahon. 1987. Expression of the proto-oncogene int-1 is restricted to specific neural cells in the developing mouse embryo. Cell 50, 79-88   DOI   ScienceOn
9 Hamburger, V. and H. 1. Hamilton. 1951. A series of normal stages in the development of the chick embryo. J. Morphol. 88, 49-92   DOI
10 Henion, P. D. and J. A. Weston. 1997. Timing and pattern of cell fate restrictions in the neural crest lineage. Development 124, 4351-4359
11 Kos R, M. V. Reedy, R 1. Johnson, and C. A Erickson. 2001. The winged-helix transcription factor FoxD3 is important for establishing the neural crest lineage and repressing melanogenesis in avian embryos. Development 128, 1467-1479
12 Hinck, 1., W. J. Nelson, and J. Papkoff. 1994. Wnt-1 modulates cell-cell adhesion in mammalian cells by stabilizing ~catenin binding to the cell adhesion protein cadherin. J. Cell BioI. 124, 729-741   DOI   ScienceOn
13 Hollyday, M., J. A. McMahon, and A. P. McMahon. 1995. Wnt expression patterns in chick embryo nervous system. Mech. Dev. 52, 9-25   DOI   ScienceOn
14 Jin, E-J., C. A. Erickson, S. Takada, and 1. W. Burrus. 2001. Wnt and BMP signaling govern lineage segregation of melanocytes in the avian embryo. Dev. BioI. 233, 22-37   DOI   ScienceOn
15 Nakagawa, S. and M. Takeichi. 1995. Neural crest cell-cell adhesion controlled by sequential and subpopulation-specific expression of novel cadherins. Development 121, 1321-1332   DOI
16 Garcia-Castro, M. I., C. Marcelle, and M. Bronner-Fraser. 2002. Ectodermal Wnt function as a neural crest inducer. Science 297, 848-851
17 Goulding, M. D., A. Lumsden, and P. Gruss. 1993. Signals from the notochord and floor plate regulate the region-specific expression of two Pax genes in the developing spinal cord. Development 117, 1001-1016
18 Nakagawa, S. and M. Takeichi. 1998. Neural crest emigration from the neural tube depends on regulated cadherin expression. Development 125, 2963-2971
19 Bienz, M. 1998. TCF: transcriptional activator or repressor? Curro Opin. Cell BioI. 10, 366-372   DOI   ScienceOn
20 Barrio, M. G. and A. Nieto. 2002. Overexpression of Snail family members highlights their ability to promote chick neural crest formation. Development 129, 1583-1593
21 Bradley, R S., P. Cowin, and A. M. Brown. 1993. Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion. J. Cell BioI. 123, 1857-1865   DOI   ScienceOn
22 Chapman, S. c., J. Collignon, G. Schownwolf, and A. Lumsden. 2001. Improved method for chick whole-embryo culture using a filter paper carrier. Dev. Dyn. 220, 284-289   DOI   ScienceOn
23 Clevers, H. and M. Van De Watering. 1997. TCF/LEF factors earn their wings. Trends Genet. 13, 485-489