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http://dx.doi.org/10.5352/JLS.2008.18.8.1090

Gene Expression Analysis of Inducible cAMP Early Repressor (ICER) Gene in Longissimus dorsi of High- and Low Marbled Hanwoo Steers  

Lee, Seung-Hwan (School of Environmental and Rural Science, The University of New England)
Kim, Nam-Kuk (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA))
Kim, Sung-Kon (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA))
Cho, Yong-Min (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA))
Yoon, Du-hak (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA))
Oh, Sung-Jong (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA))
Im, Seok-Ki (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA))
Park, Eung-Woo (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA))
Publication Information
Journal of Life Science / v.18, no.8, 2008 , pp. 1090-1095 More about this Journal
Abstract
Marbling (intramuscular fat) is an important factor in determining meat quality in Korean beef market. A grain based finishing system for improving marbling leads to inefficient meat production due to an excessive fat production. Identification of intramuscular fat-specific gene might be achieved more targeted meat production through alternative genetic improvement program such as marker assisted selection (MAS). We carried out ddRT-PCR in 12 and 27 month old Hanwoo steers and detected 300 bp PCR product of the inducible cAMP early repressor (ICER) gene, showing highly gene expression in 27 months old. A 1.5 kb sequence was re-sequenced using primer designed base on the Hanwoo EST sequence. We then predicted the open reading frame (ORF) of ICER gene in ORF finder web program. Tissue distribution of ICER gene expression was analysed in eight Hanwoo tissue using realtime PCR analysis. The highest ICER gene expression showed in Small intestine followed by Longissimus dorsi. Interestingly, the ICER gene expressed 2.5 time higher in longissimus dorsi than in same muscle type, Rump. For gene expression analysis in high- and low marbled individuals, we selected 4 and 3 animal based on the muscle crude fat contents (high is 17-32%, low is 6-7% of crude fat contents). The ICER gene expression was analysed using ANOVA model. Marbling (muscle crude fat contents) was affected by ICER gene (P=0.012). Particularly, the ICER gene expression was 4 times higher in high group (n=4) than low group (n=3). Therefore, ICER gene might be a functional candidate gene related to marbling in Hanwoo.
Keywords
Inducible cAMP early reprossor (ICER); Marbling (intramuscular fat); differential display RT-PCR (DDRT-PCR) and Analysis of Variance (ANOVA);
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1 JMGA. 1988. New beef carcass grading standards. Japan Meat Grading Association, Tokyo, Japan
2 Molina, C. A., N. S. Foulkes, E. Lalli and P. Sassone-Corsi. 1993. Inducibility and negative autoregulation of CREM: an alternative promoter directs the expression of ICER, an early response repressor. Cell 75, 875-886   DOI   ScienceOn
3 Nishimura, T., A. Hattori and K. Takahashi. 1999. Structural changes in intramuscular connective tissue during the fattening of Japanese black cattle: Effect of marbling on beef tenderization. J. Anim. Sci. 77, 93-104   DOI
4 Park, T. S., S. K. Yi, S. M. Lee, S. Y. Lee, D. H. Yoo, J. I. Ahn and Y. S. Lee. 2003. Statistical tests for identifying differentially expressed genes in time-course microarray experiments. Bioinformatics 19, 694-703   DOI   ScienceOn
5 Zhou, Y. P., K. Marlen, J. F. Palma, A. Schweitzer, L. Reilly, F. M. Gregoire, G. G. Xu, J. E. Blume and J. D. Johnson. 2003. Overexpression of repressive cAMP response element modulators in high glucose and fatty acid-treated rat islets. A common mechanism for glucose toxicity and lipotoxicity?. J. Biol. Chem. 278, 51316-51323   DOI   ScienceOn
6 Moser, R. J., A. Reverter, C. A. Kerr, K. J. Beh and S. A. Lehnert. 2004. A mixed-model approach for the analysis of cDNA microarray gene expression data from extreme- performing pigs after infection with Actinobacillus pleuropneumoniae. J. Anim. Sci. 82, 1261-1271   DOI
7 Childs, K. D., D. W. Goad, M. F. Allan, D. Pomp, C. Krehbiel, R. D. Geisert, J. B. Morgan and J. R. Malayer. 2002. Differential expression of NAT1 translational repressor during development of bovine intramuscular adipocytes. Physiol Genomics 10, 49-56   DOI
8 Kerr, M. K and G. A. Churchill. 2001. Statistical design and the analysis of gene expression microarray data. Genet. Res. Comb. 77, 123-128
9 Hussain, M. A., P. B. Daniel and J. F. Habener. 2000. Glucagon stimulates expression of the inducible cAMP early repressor and suppresses insulin gene expression in pancreatic $\beta$-cells. Diabetes 49, 1681-1690   DOI   ScienceOn
10 Wang, Y. H., K. A. Byrne, A. Reverter, G. S. Harper, M. Taniguchi, S. M. McWilliam, H. Mannen, K. Oyama and S. A. Lehnert. 2005. Transcriptional profiling of skeletal muscle tissue from two breeds of cattle. Mamm Genome 16, 201-210   DOI   ScienceOn
11 Wolfinger, R. D., G. Gibson, E. D. Wolfinger, L. Bennett, H. Hamadeh, P. Bushel, C. Afshari and R. S. Paules. 2001. Assessing gene significance from cDNA microarray expression data via mixed models. J. Comput. Biol. 8, 625-637   DOI   ScienceOn
12 Lee, S. H., E. W. Park, Y. M. Cho, S. K. Kim, J. H. Lee, J. T. Jeon, C. S. Lee, S. K. Im, S. J. Oh, J. M. Thompson and D. Yoon. 2007. Identification of differentially expressed genes related to intramuscular fat development in the early and late fattening stages of Hanwoo steers. Journal of Biochemistry and Molecular Biology 40, 757-764   DOI   ScienceOn
13 Hocquette, J. F., C. Jurie, Y. Ueda, P. Boulesteix, D. Bauchart and D. W. Pethick. 2003. The relationship between muscle metabolic pathways and marbling of beef. Progress in Research on Energy and Protein Metabolism, Wageningen, The Netherlands 1, 513-516
14 USDA. 1989. Official united states standards for grades of beef carcases. Agric. Marketing Serv. USDA, Washington, USA
15 Smith, S. B. and J. D. Crouse. 1984. Relative contributions of acetate, lactate and glucose to lipogenesis in bovine intramuscular and subcutaneous adipose tissue. J. Nutr. 114, 792-800   DOI
16 Yuan, J. S., J. Burris, N. R. Stewart, A. Mentewab and C. N. Jr. Stewart. 2007. Statistical tools for transgene copy number estimation based on real-time PCR. BMC Bioinformatics 85, 1-12
17 Casimir, D. A. and J. M. Ntambi. 1996. cAMP activates the expression of stearoyl-CoA desaturase gene 1 during early preadipocyte differentiation. J. Biol. Chem. 270, 29847-29853
18 Hu, Z., E. R. Fritz and J. M. Reecy. 2007. AnimalQTLdb: a livestock QTL database tool set for positional QTL information mining and beyond. Nucleic Acids Research 35, 604-609   DOI   ScienceOn