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http://dx.doi.org/10.5352/JLS.2008.18.8.1042

Gamma Irradiation Induces a Caspase-dependent Apoptotic Mechanism in Human Prostate Cancer PC-3 Cells  

Chang, Jeong-Hyun (Department of Clinical Laboratory Science, College of Health & Therapy, Daegu Hanny University)
Kim, Dong-Hyun (Department of Biomedical Engineering, School of Medicine, Pusan National University)
Jeon, Gye-Rok (Department of Biomedical Engineering, School of Medicine, Pusan National University)
Kwon, Heun-Young (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
Publication Information
Journal of Life Science / v.18, no.8, 2008 , pp. 1042-1048 More about this Journal
Abstract
Prostate cancer is the most predominant cancer in men and related death rate increases every year. Till date, there is no effective therapy for androgen independent prostate cancer. To investigate the mechanism for cell growth inhibition or apoptosis in human androgen independent prostate cancer PC-3 cells after gamma irradiation. The aim of this study was to examine the potential of gamma irradiation to induce apoptosis in PC-3 cells and to assess the mechanism of gamma irradiation-induced apoptosis. Five different assays were employed in this study: cell proliferation assay, morphological assessments of apoptotic cells, DNA fragmentation analysis, quantification of apoptosis by annexin V (AV) and propidium iodide (PI) staning, and western blot analysis. Cell viability was inversely related to radiation dose. DAPI-positive cells were detected 48 hr after 40 Gy radiation exposure. And nuclear morphological changes of cells were observed by gamma irradiation. DNA ladder patterns in the cells exposed to gamma-radiation were appeared at 24 hr. Also, gamma irradiation induces apoptosis of PC-3 cells via Caspase3, Bax and PARP-dependent fashion.
Keywords
Human prostate cancer PC-3 cells; gamma irradiation;
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