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http://dx.doi.org/10.5352/JLS.2008.18.7.931

Responses of Plasma IGF-1, IGFBPs and Hepatic GH Receptor to Growth Hormone Releasing Peptides (GHRP)-2 Administration and Energy Level in Wethers  

Lee, Hong-Gu (Department of Bio-Resources and Applied Life Science, Pusan National University)
Jin, Young-Cheng (Department of Agricultural Biotechnology, Seoul National University)
Hidari, Hisashi (Obihiro University of Agriculture and Veterinary Medicine)
Choi, Yun-Jaie (Department of Agricultural Biotechnology, Seoul National University)
Kim, Seon-Ku (Department of Bio-Resources and Applied Life Science, Pusan National University)
Shin, Teak-Soon (Department of Bio-Resources and Applied Life Science, Pusan National University)
Cho, Byung-Uuk (Department of Bio-Resources and Applied Life Science, Pusan National University)
Kim, Yong-Gyun (Department of Bio-Resources and Applied Life Science, Pusan National University)
Kim, Keun-Ki (Department of Bio-Resources and Applied Life Science, Pusan National University)
Son, Hong-Joo (Department of Bio-Resources and Applied Life Science, Pusan National University)
Lee, Sang-Mong (Department of Bio-Resources and Applied Life Science, Pusan National University)
Park, Hyun-Chul (Department of Bio-Resources and Applied Life Science, Pusan National University)
Kang, Han-Seok (Department of Bio-Resources and Applied Life Science, Pusan National University)
Publication Information
Journal of Life Science / v.18, no.7, 2008 , pp. 931-939 More about this Journal
Abstract
The purpose of this study was to determine the effect of energy supplement on responses of plasma insulin-like growth factor (IGF)-1 and IGF binding proteins (IGFBPs) to growth hormone-releasing peptide-2 (GHRP-2) administration in normal protein-fed wethers, and to observe the effect of GHRP-2 treatment on hepatic growth hormone (GH) receptor in well-fed wethers. Plasma IGF-1 and 39-42 kDa IGFBP-3 during the HENP (CP, crude protein 0.34 and TDN, total digestible nutrients 1.83 kg/day DM, dry matter intake) treatment period were higher than in the LENP (CP 0.32 kg and TDN 0.87 kg/day DM intake) period (P<0.05). The response of GH was stimulated by GHRP-2 ($12.5\;{\mu}g/kg$ body weight/day) administration during both of the feed treatment periods (P<0.05). The area under curve (AUC) increment and average concentration of GH (0-180 min) with GHRP-2 administration was higher during HENP treatment than LENP treatment (P<0.01). During the HENP treatment period from day 1 to day 7 of twice daily GHRP-2 treatment, the plasma IGF-1 increment was increased on days 2, 6 and 7 of GHRP-2 administration (P<0.05). On the basis of ligand blotting, the proportions of plasma 39-43 kDa IGFBP-3 during the HENP treatment period only showed a significant difference on days 6 and 7 with GHRP-2 administration. No significant difference in the specific binding of $^{125}I-labeled$ oGH to hepatic membranes was detected between the saline and GHRP-2 treatments of the HENP-fed wethers. These results suggest that the nutritional balance between energy and protein may affect the endogenous GH / IGF-1 axis as well as plasma IGFBP-3 levels.
Keywords
Dietary energy; growth hormone-releasing peptide-2 (GHRP-2); insulin-like growth factor-1 (IGF-1); IGF binding proteins (IGFBPs); wether;
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