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http://dx.doi.org/10.5352/JLS.2008.18.7.1005

Inhibitory Effects of Four Solvent Fractions of Alnus firma on α-Amylase and α-Glucosidase.  

Choi, Hye-Jung (Interdisciplinary Program in Biotechnology, Changwon National University)
Jeong, Yong-Kee (Department of Biotechnology, Dong-A University, Department Cioinstitue, MILLENNIUM PROMISE CO., LTD.)
Kang, Dae-Ook (Department of Biochemistry and Health Sciences, Changwon National University)
Joo, Woo-Hong (Department of Biology, Changwon National University)
Publication Information
Journal of Life Science / v.18, no.7, 2008 , pp. 1005-1010 More about this Journal
Abstract
In this study, we investigated the inhibitory effect of four solvent fractions of Alnus firma on ${\alpha}-amylase$, ${\alpha}-glucosidase$ and aldose reductase activities. The inhibitory test showed that methanol (MeOH) extract and hexane (HX) fraction strongly inhibited pork pancreatin and salivary ${\alpha}-amylase$ activity. The MeOH extract and HX fraction of Alnus firma at the concentration of 4 mg/ml inhibited more than 70% of pancreatin and salivary ${\alpha}-amylase$ activity. The inhibitory effect of fractions has different specificities against ${\alpha}-amylase$ from pancreatin and salivary. In addition, the MeOH extract and butanol (BuOH) fraction showed the highest inhibitory activity on yeast ${\alpha}-glucosidase$ at values of $IC_{50}$ $137.36\;{\mu}g/ml$ and $115.14\;{\mu}g/ml$ respectively. The MeOH extract and BuOH fraction showed the highest inhibitory activity on yeast ${\alpha}-glucosidase$ than commercial agent such as 1-deoxynorjirimycin and acarbose. Inhibition kinetics of solvent fractions showed that ${\alpha}-glucosidase$ has been inhibited noncompetitively by the MeOH, EA and BuOH fraction. The aldose reductase from human muscle cell had been inhibited strongly by the MeOH extract and EA fraction at 57.996% and 83.293% at the concentration of $50\;{\mu}g/ml$, respectively. These findings may contribute to biological significance in that ${\alpha}-amylase$, ${\alpha}-glucosidase$ and aldose reductase inhibitory compounds could be used as a functional food and a drug for the symptomatic treatment of antidiabetic disease in the future.
Keywords
Alnus firma; solvent fractions${\alpha}-amylase$${\alpha}-glucosidase$; aldose reductase; inhibitory effects;
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