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http://dx.doi.org/10.5352/JLS.2008.18.6.884

Genistein-induced Growth Inhibition was Associated with Inhibition of Cyclooxygenase-2 and Telomerase Activity in Human Cancer Cells.  

Kim, Jung-Im (Department of Biochemistry, Dongeui University College of Oriental Medicine)
Kim, Seong-Yun (Department of Biomaterial Control (BK21 program), Dongeui University Graduate School)
Seo, Min-Jeong (Department of Biotechnology, College of Natural Resources and Life Science and BK21 Center for Silver-Bio Industrialization, Dong-A University)
Lim, Hak-Seob (Bioinstitute, Millennium Promise Co., LTD)
Lee, Young-Choon (Department of Biotechnology, College of Natural Resources and Life Science and BK21 Center for Silver-Bio Industrialization, Dong-A University)
Joo, Woo-Hong (Department of Biology, Changwon National University)
Choi, Byung-Tae (Department of Anatomy, Graduate School of Oriental Medicine, Pusan National University)
Jeong, Yong-Kee (Department of Biotechnology, College of Natural Resources and Life Science and BK21 Center for Silver-Bio Industrialization, Dong-A University)
Choi, Yung-Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine, Department of Biomaterial Control (BK21 program), Dongeui University Graduate School)
Publication Information
Journal of Life Science / v.18, no.6, 2008 , pp. 884-890 More about this Journal
Abstract
Genistein, an isoflavone in soybean products, is a potential chemopreventive agent against various types of cancer. There are several studies documenting molecular alterations leading to cell cycle arrest at G2/M phase and induction of apoptosis; however, its mechanism of action and its molecular targets on the prostaglandin $E_2$ ($PGE_2$) production and telomere length regulation in human cancer remain unclear. In this study, we investigated the effect of genistein on the levels of cyclooxygenases (COXs) and telomere regulatory components of several human cancer cell lines (T24, human bladder carcinoma cells; U937, human leukemic cells; AGS, human stomach adenocarcinoma cells and SK-MEL-2, human skin melanoma cells). Genistein treatment resulted in the inhibition of cancer cell proliferation in a concentration-dependent manner. It was found that genistein treatment markedly decreased the levels of COX-2 mRNA and protein expression without significant changes in the expression of COX-1, which was correlated with a decrease in $PGE_2$ synthesis. Genistein treatment also partly inhibited the levels of human telomerase reverse transcriptase (hTERT) as well as human telomerase RNA (hTR) and telomerase-associated protein (TEP)-1, and the activity of telomerase. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of genistein.
Keywords
Genistein; cancer; cyclooxygenase-2; telomerase;
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