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http://dx.doi.org/10.5352/JLS.2008.18.6.839

Optimal Culture Conditions on the Keratinase Production by Bacillus sp. SH-517.  

Bang, Byung-Ho (Division of Food Science, Eulji University)
Rhee, Moon-Soo (Korea Research Institute of Bioscience and Biotechnology)
Lim, Ki-Hwan (Department of Microbial Engineering, Konkuk University)
Yi, Dong-Heui (Department of Microbial Engineering, Konkuk University)
Publication Information
Journal of Life Science / v.18, no.6, 2008 , pp. 839-844 More about this Journal
Abstract
A strain SH-517 which produce extracellular keratinase, was isolated from the soil of a poultry waste and a poultry factory. An isolate SH-517 was identified as Bacillus sp. based on its morphological and biochemical characteristics. The optimal culture conditions for the production of keratinase by Bacillus sp. SH-517 were investigated. The optimal medium composition for keratinase production was determined to be 2.0% chicken feather as carbon source, 0.5% beef extract as organic nitrogen source, 0.5% $KNO_3$ as inorganic nitrogen source and 0.06% KCl, 0.05% NaCl, 0.04% $KH_2PO_4$, 0.03% $K_2HPO_4$ as mineral source and 0.01% yeast extract as growth factor. The optimal temperature and pH of medium were shown $40^{\circ}C$ and 8.5 with shaking culture (180 rpm/min), respectively. The maximum keratinase production reached maximum of 125 units/ml/min after 42 hr of cultivation under the optimal culturing conditions.
Keywords
Keratinase; chicken feather; production; Bacillus sp. SH-517;
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