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http://dx.doi.org/10.5352/JLS.2008.18.4.542

The Effects of Pro-inflammatory Cytokines by Cisplatin on the Death of Sensory Hair Cells.  

Lee, Jeong-Han (Department of Microbiology & Vestibulocochlear Research Center, Wonkwang University College of Medicine)
Park, Chan-Ny (Department of Microbiology & Vestibulocochlear Research Center, Wonkwang University College of Medicine)
Park, Rae-Kil (Department of Microbiology & Vestibulocochlear Research Center, Wonkwang University College of Medicine)
Publication Information
Journal of Life Science / v.18, no.4, 2008 , pp. 542-549 More about this Journal
Abstract
Cisplatin (cis-diamminedichloroplatinum II : CDDP) is the most widely used anticancer drug against a variety of human neoplasms. However, its clinical use is limited by the onset of severe side effects, including ototoxicity and nephrotoxicity. Even though a number of evidences in cytotoxic mechanism of cisplatin have been suggested, the role of pro-inflammatory cytokines in cisplatin cytotoxicity of auditory cells has not yet been demonstrated. Herein our data clearly demonstrated that cisplatin decreased the viability of HEI-OC1 auditory cells, which was inhibited by the addition of neutralizing $anti-TNF-{\alpha}$, $anti-IL-1{\beta}$ and anti-IL-6 antibodies. Consistently, Neutralization with antibodies against pro-inflammatory cytokines ameliorated the cell death and disarrangement of cochlea hair cell layers in the rat primary cochlear explants which were treated with cisplatin. Furthermore, exogeneous supplementation with free radical scavengers, including GSH and NAC, significantly prevented the cytotoxicity of cisplatin in the rat primary cochlea explants. We also observed that $TNF-{\alpha}$ was predominantly expressed in Deiters and Hensen's cells located in hair cell zone of cisplatin-treated cochlear explants. These findings suggest that pro-inflammatory cytokines, including $TNF-{\alpha}$, $IL-1{\beta}$ and IL-6, may play a pivotal role in the pathophysiology of hair cell damages caused by ototoxic drug cisplatin.
Keywords
Cisplatin; cytokine; hair cell; ototoxicity; antioxidant;
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1 So, H. S., C. Park, H. J. Kim, J. H. Lee, S. Y. Park, J. H. Lee, Z. W. Lee, H. M. Kim, F. Kalinec, D. J. Lim and R. Park. 2005. Protective effect of T-type calcium channel blocker flunarizine on cisplatin-induced death of auditory cells. Hearing Research 204, 127-139   DOI   ScienceOn
2 So, H., H. Kim, J. H. Lee, C. Park, Y. Kim, E. Kim, J. K. Kim, K. J. Yun, K. M. Lee, H. Y. Lee, S. K. Moon, D. J. Lim and R. Park. 2007. Cisplatin cytotoxicity of auditory cells requires secretions of proinflammatory cytokines via activation of ERK and NF-kappaB. J. Assoc. Res. Otolaryngol. 8, 338-355   DOI   ScienceOn
3 Strock, M., M. Schilling, K. Burkhardt, R. Prestel, D. Abendroth and C. Hamme. 1994. Production of proinflammatory cytokine and adhension molecules in ex-vivo xenogeneic Kidney perfusion. Transpl. Int. 1, S647-S649
4 Teranishi, M., T. Nakashima and T. Wakabayashi. 2001. Effects of alphatocopherol on cisplatin-induced ototoxicity in guinea pigs. Hearing Research 151, 61-70   DOI   ScienceOn
5 Yang, C. S., H. M. Lee, J. Y. Lee, J. A. Kim, S. J. Lee, D. M. Shin, Y. H. Lee, D. S. Lee, J. El-Benna and E. K. Jo. 2007. Reactive oxygen species and p47phox activation are essential for the Mycobacterium tuberculosis-induced pro-inflammatory response in murine microglia. J. Neuroinflammation 4, 27   DOI   ScienceOn
6 Laurell, G. and S. D. Batter. 1991. Degeneration of the organ of Corti following intravenous administration of cisplatin. Acta. Otolaryngol. 111, 891-898   DOI   ScienceOn
7 Kopke, R. D., W. Liu, R. Gabaizad, A. Jacono, J. Feghali, D. Spray, P. Garcia, H. Steinman, B. Malgrange, R. J. Ruben, L. Rybak and T. R. Van De Water. 1997. Use of organotypic cultures of Corti's organ to study the protective effects of antioxidant molecules on cisplatin-induced damage of auditory hair cells. AM. J. Otol. 18, 559-571
8 Kartalou, M. and J. M. Essigmann. 2001. Mechanism of resistance to cisplatin. Mutat. Res. 478, 23-43   DOI   ScienceOn
9 Kathleen, C. M., L. P. Campbell, R. P. Rybak and L. H. Meech. 1996. D-Methionine provides excellent protection from cisplatin ototoxicity in the rat. Hearing Research 102, 90-98   DOI   ScienceOn
10 Hikiji, H., W. S. Shin, T. Koizumi, T. Takato, T. Susami, Y. Koizumi, Y. Okai-Matsuo and T. Toyo-Oka. 2000. Peroxynitrite production by TNF-alpha and IL-1beta: implication for suppression of osteoblastic differentiation. Am. J. Physiol. Endocrinol. Metab. 278, E1031-E1037   DOI
11 Jordan, P. and M. Carmo-Fonseca. 2000. Molecular mechanism involved in cisplatin cytotoxicity. Cell Mol. Life Sci. 57, 1229-1235   DOI   ScienceOn
12 Kalinec, G. M., P. Webster, D. J. Lim and F. Kalinec. 2003. A cochlear cell line as an in vitro system for drug ototoxicity screening. Audiol. Neurootol. 8, 177-189   DOI   ScienceOn
13 Zhang, B., G. Ramesh, C. C. Norbury and W. B. Reeves. 2007. Cisplatin-induced nephrotoxicity is mediated by tumor necrosis factor-alpha produced by renal parenchymal cells. Kidney Int. 72, 37-44   DOI   ScienceOn
14 Watanabe, K. C., K. Jinnouchi, A. Hess, O. Michel, S. Baba and T. Yagi. 2002. Carboplatin induces less apoptosis in the cochlea of guinea pigs than cisplatin. Chemotherapy 48, 82-87   DOI   ScienceOn
15 Feghali, J. G., W. Liu and T. R. Van De Water. 2001. L-n-acetyl-cysteine protection against cisplatin-induced auditory neuronal and hair cell toxicity. Laryngoscope. 111, 1147-1155
16 Fujinobu, T., C. A. Whitworth and L. P. Rybak. 2003. Influence of PH on the ototoxicity of cisplatin: a round window application study. Hearing Research 177, 21-31   DOI   ScienceOn
17 Ding, D., A. Stracher and R. J. Salvi. 2002. Leupeptin protects cochlear and vestibular hair cells from gentamicin ototoxicity. Hearing Research 164, 115-126   DOI   ScienceOn
18 Alam, S. A., K. Ikeda, T. Oshima, M. Suzuki, T. Kawase, T. Kikuchi and T. Takasaka. 2000. Cisplatin-induced apoptotic cell death in Mongolian gerbil cochlea. Hearing Research 141, 28-38   DOI   ScienceOn
19 Estrem, S. A., R. W. Babin, J. H. Ryu and K. C. Moore. 1981. Cis-diamminedichloroplatinum (II) ototoxicity in the guinea pig. Otolaryngol. Head. Neck. Surg. 90, 638-645
20 Farm, R. J. 1992. Cisplatin and platinum analogues: recent advances. Curr. Opin. Oncol. 4, 1073-1079   DOI   ScienceOn
21 Brueckmann, M., T. Bertsch, S. Lang, T. Sueselbeck, C. Wlopert, J. J. Kaden, C. Jaramillo, G. Huhel, M. Borggrefe and K. K. Haase. 2004. Time course of systemic markers of inflammation in patients presenting with acute coronary syndromes. Clin. Chem. Lab. Med. 42, 1132-1139   DOI   ScienceOn
22 Prasad, D., S. Michelle, M. C. Patricia, M. S. Park, E. C. Nora, G. Kalinec and F. Kalinec. 2002. Cisplatin-induced apoptois in auditory cells: role of dath receptor and mitochondrial pathways. Hearing Research 174, 45-54   DOI   ScienceOn
23 Rybak L. P., K. Husain, C. Whitworth and S. M. Somani. 1999. Dose dependent protection by lipoic acid against cisplatin- induced ototoxicity in rats: Antioxodant defense system. Toxicological Sciences 47, 195-202   DOI   ScienceOn